IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-27297-2.html
   My bibliography  Save this article

Siderophore-mediated zinc acquisition enhances enterobacterial colonization of the inflamed gut

Author

Listed:
  • Judith Behnsen

    (University of California Irvine
    University of Illinois Chicago)

  • Hui Zhi

    (University of California San Diego)

  • Allegra T. Aron

    (University of California San Diego
    University of California, San Diego)

  • Vivekanandan Subramanian

    (University of Kentucky PharmNMR Center, College of Pharmacy, University of Kentucky)

  • William Santus

    (University of Illinois Chicago)

  • Michael H. Lee

    (University of California San Diego)

  • Romana R. Gerner

    (University of California San Diego)

  • Daniel Petras

    (University of California San Diego
    University of California, San Diego)

  • Janet Z. Liu

    (University of California Irvine)

  • Keith D. Green

    (University of Kentucky)

  • Sarah L. Price

    (University of Louisville School of Medicine)

  • Jose Camacho

    (University of California San Diego)

  • Hannah Hillman

    (University of California San Diego)

  • Joshua Tjokrosurjo

    (University of California Irvine)

  • Nicola P. Montaldo

    (University of California Irvine)

  • Evelyn M. Hoover

    (University of California Irvine)

  • Sean Treacy-Abarca

    (University of California Irvine)

  • Benjamin A. Gilston

    (Vanderbilt University Medical Center)

  • Eric P. Skaar

    (Vanderbilt University Medical Center)

  • Walter J. Chazin

    (Vanderbilt University Medical Center)

  • Sylvie Garneau-Tsodikova

    (University of Kentucky)

  • Matthew B. Lawrenz

    (University of Louisville School of Medicine)

  • Robert D. Perry

    (University of Kentucky)

  • Sean-Paul Nuccio

    (University of California Irvine
    University of California San Diego)

  • Pieter C. Dorrestein

    (University of California San Diego
    University of California, San Diego
    University of California San Diego)

  • Manuela Raffatellu

    (University of California Irvine
    University of California San Diego
    University of California San Diego
    Chiba University-UC San Diego Center for Mucosal Immunology, Allergy, and Vaccines (CU-UCSD cMAV))

Abstract

Zinc is an essential cofactor for bacterial metabolism, and many Enterobacteriaceae express the zinc transporters ZnuABC and ZupT to acquire this metal in the host. However, the probiotic bacterium Escherichia coli Nissle 1917 (or “Nissle”) exhibits appreciable growth in zinc-limited media even when these transporters are deleted. Here, we show that Nissle utilizes the siderophore yersiniabactin as a zincophore, enabling Nissle to grow in zinc-limited media, to tolerate calprotectin-mediated zinc sequestration, and to thrive in the inflamed gut. We also show that yersiniabactin’s affinity for iron or zinc changes in a pH-dependent manner, with increased relative zinc binding as the pH increases. Thus, our results indicate that siderophore metal affinity can be influenced by the local environment and reveal a mechanism of zinc acquisition available to commensal and pathogenic Enterobacteriaceae.

Suggested Citation

  • Judith Behnsen & Hui Zhi & Allegra T. Aron & Vivekanandan Subramanian & William Santus & Michael H. Lee & Romana R. Gerner & Daniel Petras & Janet Z. Liu & Keith D. Green & Sarah L. Price & Jose Camac, 2021. "Siderophore-mediated zinc acquisition enhances enterobacterial colonization of the inflamed gut," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27297-2
    DOI: 10.1038/s41467-021-27297-2
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-27297-2
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-27297-2?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Franziska Faber & Lisa Tran & Mariana X. Byndloss & Christopher A. Lopez & Eric M. Velazquez & Tobias Kerrinnes & Sean-Paul Nuccio & Tamding Wangdi & Oliver Fiehn & Renée M. Tsolis & Andreas J. Bäumle, 2016. "Host-mediated sugar oxidation promotes post-antibiotic pathogen expansion," Nature, Nature, vol. 534(7609), pages 697-699, June.
    2. Martina Sassone-Corsi & Sean-Paul Nuccio & Henry Liu & Dulcemaria Hernandez & Christine T. Vu & Amy A. Takahashi & Robert A. Edwards & Manuela Raffatellu, 2016. "Microcins mediate competition among Enterobacteriaceae in the inflamed gut," Nature, Nature, vol. 540(7632), pages 280-283, December.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Ana Carolina Dantas Machado & Stephany Flores Ramos & Julia M. Gauglitz & Anne-Marie Fassler & Daniel Petras & Alexander A. Aksenov & Un Bi Kim & Michael Lazarowicz & Abbey Barnard Giustini & Hamed Ar, 2023. "Portosystemic shunt placement reveals blood signatures for the development of hepatic encephalopathy through mass spectrometry," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Ana Djukovic & María José Garzón & Cécile Canlet & Vitor Cabral & Rym Lalaoui & Marc García-Garcerá & Julia Rechenberger & Marie Tremblay-Franco & Iván Peñaranda & Leonor Puchades-Carrasco & Antonio P, 2022. "Lactobacillus supports Clostridiales to restrict gut colonization by multidrug-resistant Enterobacteriaceae," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    2. Courtney Hoskinson & Darlene L. Y. Dai & Kate L. Bel & Allan B. Becker & Theo J. Moraes & Piushkumar J. Mandhane & B. Brett Finlay & Elinor Simons & Anita L. Kozyrskyj & Meghan B. Azad & Padmaja Subba, 2023. "Delayed gut microbiota maturation in the first year of life is a hallmark of pediatric allergic disease," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27297-2. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.