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Towards non-blind optical tweezing by finding 3D refractive index changes through off-focus interferometric tracking

Author

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  • Benjamin Landenberger

    (University of Freiburg
    BIOSS Centre for Biological Signalling Studies, University of Freiburg)

  • Yatish

    (University of Freiburg
    CIBSS - Centre for Integrative Biological Signalling Studies
    University of Freiburg)

  • Alexander Rohrbach

    (University of Freiburg
    BIOSS Centre for Biological Signalling Studies, University of Freiburg
    CIBSS - Centre for Integrative Biological Signalling Studies)

Abstract

In modern 3D microscopy, holding and orienting arbitrary biological objects with optical forces instead of using coverslips and gel cylinders is still a vision. Although optical trapping forces are strong enough and related photodamage is acceptable, the precise (re-) orientation of large specimen with multiple optical traps is difficult, since they grab blindly at the object and often slip off. Here, we present an approach to localize and track regions with increased refractive index using several holographic optical traps with a single camera in an off-focus position. We estimate the 3D grabbing positions around several trapping foci in parallel through analysis of the beam deformations, which are continuously measured by defocused camera images of cellular structures inside cell clusters. Although non-blind optical trapping is still a vision, this is an important step towards fully computer-controlled orientation and feature-optimized laser scanning of sub-mm sized biological specimen for future 3D light microscopy.

Suggested Citation

  • Benjamin Landenberger & Yatish & Alexander Rohrbach, 2021. "Towards non-blind optical tweezing by finding 3D refractive index changes through off-focus interferometric tracking," Nature Communications, Nature, vol. 12(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27262-z
    DOI: 10.1038/s41467-021-27262-z
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    References listed on IDEAS

    as
    1. Frederic Berndt & Gopi Shah & Rory M. Power & Jan Brugués & Jan Huisken, 2018. "Dynamic and non-contact 3D sample rotation for microscopy," Nature Communications, Nature, vol. 9(1), pages 1-7, December.
    2. Robin Diekmann & Deanna L. Wolfson & Christoph Spahn & Mike Heilemann & Mark Schüttpelz & Thomas Huser, 2016. "Nanoscopy of bacterial cells immobilized by holographic optical tweezers," Nature Communications, Nature, vol. 7(1), pages 1-7, December.
    3. Kyoohyun Kim & YongKeun Park, 2017. "Tomographic active optical trapping of arbitrarily shaped objects by exploiting 3D refractive index maps," Nature Communications, Nature, vol. 8(1), pages 1-8, August.
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