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Nanoscopy of bacterial cells immobilized by holographic optical tweezers

Author

Listed:
  • Robin Diekmann

    (Biomolecular Photonics, University of Bielefeld)

  • Deanna L. Wolfson

    (NSF Center for Biophotonics, University of California
    UiT The Arctic University of Norway)

  • Christoph Spahn

    (Institute of Physical and Theoretical Chemistry, Johann Wolfgang Goethe-University Frankfurt)

  • Mike Heilemann

    (Institute of Physical and Theoretical Chemistry, Johann Wolfgang Goethe-University Frankfurt)

  • Mark Schüttpelz

    (Biomolecular Photonics, University of Bielefeld)

  • Thomas Huser

    (Biomolecular Photonics, University of Bielefeld
    NSF Center for Biophotonics, University of California)

Abstract

Imaging non-adherent cells by super-resolution far-field fluorescence microscopy is currently not possible because of their rapid movement while in suspension. Holographic optical tweezers (HOTs) enable the ability to freely control the number and position of optical traps, thus facilitating the unrestricted manipulation of cells in a volume around the focal plane. Here we show that immobilizing non-adherent cells by optical tweezers is sufficient to achieve optical resolution well below the diffraction limit using localization microscopy. Individual cells can be oriented arbitrarily but preferably either horizontally or vertically relative to the microscope’s image plane, enabling access to sample sections that are impossible to achieve with conventional sample preparation and immobilization. This opens up new opportunities to super-resolve the nanoscale organization of chromosomal DNA in individual bacterial cells.

Suggested Citation

  • Robin Diekmann & Deanna L. Wolfson & Christoph Spahn & Mike Heilemann & Mark Schüttpelz & Thomas Huser, 2016. "Nanoscopy of bacterial cells immobilized by holographic optical tweezers," Nature Communications, Nature, vol. 7(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13711
    DOI: 10.1038/ncomms13711
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    Cited by:

    1. Benjamin Landenberger & Yatish & Alexander Rohrbach, 2021. "Towards non-blind optical tweezing by finding 3D refractive index changes through off-focus interferometric tracking," Nature Communications, Nature, vol. 12(1), pages 1-11, December.
    2. Ye Yang & Yaozhang Yang & Dingyuan Liu & Yuanyuan Wang & Minqiao Lu & Qi Zhang & Jiqing Huang & Yongchuan Li & Teng Ma & Fei Yan & Hairong Zheng, 2023. "In-vivo programmable acoustic manipulation of genetically engineered bacteria," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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