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Dynamic and non-contact 3D sample rotation for microscopy

Author

Listed:
  • Frederic Berndt

    (Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108
    Max Planck Institute for the Physics of Complex Systems, Nöthnitzer Str. 38
    Center for Systems Biology Dresden, Pfotenhauerstr. 108)

  • Gopi Shah

    (Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108
    European Molecular Biology Laboratory)

  • Rory M. Power

    (Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108
    Morgridge Institute for Research)

  • Jan Brugués

    (Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108
    Max Planck Institute for the Physics of Complex Systems, Nöthnitzer Str. 38
    Center for Systems Biology Dresden, Pfotenhauerstr. 108)

  • Jan Huisken

    (Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108
    Morgridge Institute for Research)

Abstract

Precise sample orientation is crucial for microscopy but is often performed with macroscopic tools and low accuracy. In vivo imaging of growing and developing samples even requires dynamic adaptation of the sample orientation to continuously achieve optimal imaging. Here, we present a method for freely positioning a sample in 3D by introducing magnetic beads and applying a magnetic field. We demonstrate magnetic orientation of fixed mouse embryos and artemia, and live zebrafish embryos and larvae on an epi-fluorescence microscope and on a light-sheet system for optimal imaging.

Suggested Citation

  • Frederic Berndt & Gopi Shah & Rory M. Power & Jan Brugués & Jan Huisken, 2018. "Dynamic and non-contact 3D sample rotation for microscopy," Nature Communications, Nature, vol. 9(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-07504-3
    DOI: 10.1038/s41467-018-07504-3
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    Cited by:

    1. Benjamin Landenberger & Yatish & Alexander Rohrbach, 2021. "Towards non-blind optical tweezing by finding 3D refractive index changes through off-focus interferometric tracking," Nature Communications, Nature, vol. 12(1), pages 1-11, December.

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