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Initiation of cytosolic plant purine nucleotide catabolism involves a monospecific xanthosine monophosphate phosphatase

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  • Katharina J. Heinemann

    (Leibniz Universität Hannover, Department of Molecular Nutrition and Biochemistry of Plants)

  • Sun-Young Yang

    (Seoul National University, Department of Agricultural Biotechnology)

  • Henryk Straube

    (Leibniz Universität Hannover, Department of Molecular Nutrition and Biochemistry of Plants)

  • Nieves Medina-Escobar

    (Leibniz Universität Hannover, Department of Molecular Nutrition and Biochemistry of Plants)

  • Marina Varbanova-Herde

    (Leibniz Universität Hannover, Department of Molecular Nutrition and Biochemistry of Plants)

  • Marco Herde

    (Leibniz Universität Hannover, Department of Molecular Nutrition and Biochemistry of Plants)

  • Sangkee Rhee

    (Seoul National University, Department of Agricultural Biotechnology)

  • Claus-Peter Witte

    (Leibniz Universität Hannover, Department of Molecular Nutrition and Biochemistry of Plants)

Abstract

In plants, guanosine monophosphate (GMP) is synthesized from adenosine monophosphate via inosine monophosphate and xanthosine monophosphate (XMP) in the cytosol. It has been shown recently that the catabolic route for adenylate-derived nucleotides bifurcates at XMP from this biosynthetic route. Dephosphorylation of XMP and GMP by as yet unknown phosphatases can initiate cytosolic purine nucleotide catabolism. Here we show that Arabidopsis thaliana possesses a highly XMP-specific phosphatase (XMPP) which is conserved in vascular plants. We demonstrate that XMPP catalyzes the irreversible entry reaction of adenylate-derived nucleotides into purine nucleotide catabolism in vivo, whereas the guanylates enter catabolism via an unidentified GMP phosphatase and guanosine deaminase which are important to maintain purine nucleotide homeostasis. We also present a crystal structure and mutational analysis of XMPP providing a rationale for its exceptionally high substrate specificity, which is likely required for the efficient catalysis of the very small XMP pool in vivo.

Suggested Citation

  • Katharina J. Heinemann & Sun-Young Yang & Henryk Straube & Nieves Medina-Escobar & Marina Varbanova-Herde & Marco Herde & Sangkee Rhee & Claus-Peter Witte, 2021. "Initiation of cytosolic plant purine nucleotide catabolism involves a monospecific xanthosine monophosphate phosphatase," Nature Communications, Nature, vol. 12(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27152-4
    DOI: 10.1038/s41467-021-27152-4
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    References listed on IDEAS

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    1. Philip Pallmann & Ludwig A. Hothorn, 2016. "Analysis of means: a generalized approach using R," Journal of Applied Statistics, Taylor & Francis Journals, vol. 43(8), pages 1541-1560, June.
    2. Esther Herberich & Johannes Sikorski & Torsten Hothorn, 2010. "A Robust Procedure for Comparing Multiple Means under Heteroscedasticity in Unbalanced Designs," PLOS ONE, Public Library of Science, vol. 5(3), pages 1-8, March.
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    Cited by:

    1. Luisa Voß & Katharina J. Heinemann & Marco Herde & Nieves Medina-Escobar & Claus-Peter Witte, 2022. "Enzymes and cellular interplay required for flux of fixed nitrogen to ureides in bean nodules," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

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