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Single-cell analysis of diverse immune phenotypes in malignant pleural effusion

Author

Listed:
  • Zhong-Yin Huang

    (Capital Medical University)

  • Ming-Ming Shao

    (Capital Medical University)

  • Jian-Chu Zhang

    (Huazhong University of Science and Technology)

  • Feng-Shuang Yi

    (Capital Medical University)

  • Juan Du

    (Capital Medical University)

  • Qiong Zhou

    (Huazhong University of Science and Technology)

  • Feng-Yao Wu

    (Nanning Fourth People’s Hospital)

  • Sha Li

    (Nanning Fourth People’s Hospital)

  • Wei Li

    (Nanning Fourth People’s Hospital)

  • Xian-Zhen Huang

    (Nanning Fourth People’s Hospital)

  • Kan Zhai

    (Capital Medical University)

  • Huan-Zhong Shi

    (Capital Medical University)

Abstract

The complex interactions among different immune cells have important functions in the development of malignant pleural effusion (MPE). Here we perform single-cell RNA sequencing on 62,382 cells from MPE patients induced by non-small cell lung cancer to describe the composition, lineage, and functional states of infiltrating immune cells in MPE. Immune cells in MPE display a number of transcriptional signatures enriched for regulatory T cells, B cells, macrophages, and dendritic cells compared to corresponding counterparts in blood. Helper T, cytotoxic T, regulatory T, and T follicular helper cells express multiple immune checkpoints or costimulatory molecules. Cell-cell interaction analysis identifies regulatory B cells with more interactions with CD4+ T cells compared to CD8+ T cells. Macrophages are transcriptionally heterogeneous and conform to M2 polarization characteristics. In addition, immune cells in MPE show the general up-regulation of glycolytic pathways associated with the hypoxic microenvironment. These findings show a detailed atlas of immune cells in human MPE and enhance the understanding of potential diagnostic and therapeutic targets in advanced non-small cell lung cancer.

Suggested Citation

  • Zhong-Yin Huang & Ming-Ming Shao & Jian-Chu Zhang & Feng-Shuang Yi & Juan Du & Qiong Zhou & Feng-Yao Wu & Sha Li & Wei Li & Xian-Zhen Huang & Kan Zhai & Huan-Zhong Shi, 2021. "Single-cell analysis of diverse immune phenotypes in malignant pleural effusion," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27026-9
    DOI: 10.1038/s41467-021-27026-9
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    References listed on IDEAS

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    1. Zhengtao Xiao & Ziwei Dai & Jason W. Locasale, 2019. "Metabolic landscape of the tumor microenvironment at single cell resolution," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
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    Cited by:

    1. Rebekka Wegmann & Lorenz Bankel & Yasmin Festl & Kate Lau & Sohyon Lee & Fabian Arnold & Valentina Cappelletti & Aaron Fehr & Paola Picotti & Konstantin J. Dedes & Daniel Franzen & Daniela Lenggenhage, 2024. "Molecular and functional landscape of malignant serous effusions for precision oncology," Nature Communications, Nature, vol. 15(1), pages 1-21, December.

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