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A common allosteric mechanism regulates homeostatic inactivation of auxin and gibberellin

Author

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  • Sayaka Takehara

    (Nagoya University)

  • Shun Sakuraba

    (National Institutes for Quantum and Radiological Science and Technology)

  • Bunzo Mikami

    (Kyoto University)

  • Hideki Yoshida

    (Nagoya University)

  • Hisako Yoshimura

    (Nagoya University)

  • Aya Itoh

    (Nagoya University)

  • Masaki Endo

    (National Agriculture and Food Research Organization)

  • Nobuhisa Watanabe

    (Nagoya University)

  • Takayuki Nagae

    (Nagoya University)

  • Makoto Matsuoka

    (Nagoya University)

  • Miyako Ueguchi-Tanaka

    (Nagoya University)

Abstract

Allosteric regulation is protein activation by effector binding at a site other than the active site. Here, we show via X-ray structural analysis of gibberellin 2-oxidase 3 (GA2ox3), and auxin dioxygenase (DAO), that such a mechanism maintains hormonal homeostasis in plants. Both enzymes form multimers by interacting via GA4 and indole-3-acetic acid (IAA) at their binding interface. Via further functional analyses we reveal that multimerization of these enzymes gradually proceeds with increasing GA4 and IAA concentrations; multimerized enzymes have higher specific activities than monomer forms, a system that should favour the maintenance of homeostasis for these phytohormones. Molecular dynamic analysis suggests a possible mechanism underlying increased GA2ox3 activity by multimerization—GA4 in the interface of oligomerized GA2ox3s may be able to enter the active site with a low energy barrier. In summary, homeostatic systems for maintaining GA and IAA levels, based on a common allosteric mechanism, appear to have developed independently.

Suggested Citation

  • Sayaka Takehara & Shun Sakuraba & Bunzo Mikami & Hideki Yoshida & Hisako Yoshimura & Aya Itoh & Masaki Endo & Nobuhisa Watanabe & Takayuki Nagae & Makoto Matsuoka & Miyako Ueguchi-Tanaka, 2020. "A common allosteric mechanism regulates homeostatic inactivation of auxin and gibberellin," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16068-0
    DOI: 10.1038/s41467-020-16068-0
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    Cited by:

    1. Suining Deng & Siqi Jiang & Baoshen Liu & Tao Zhong & Qingcai Liu & Jianju Liu & Yuanliang Liu & Can Yin & Chen Sun & Mingliang Xu, 2024. "ZmGDIα-hel counters the RBSDV-induced reduction of active gibberellins to alleviate maize rough dwarf virus disease," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    2. Ken-ichiro Hayashi & Kazushi Arai & Yuki Aoi & Yuka Tanaka & Hayao Hira & Ruipan Guo & Yun Hu & Chennan Ge & Yunde Zhao & Hiroyuki Kasahara & Kosuke Fukui, 2021. "The main oxidative inactivation pathway of the plant hormone auxin," Nature Communications, Nature, vol. 12(1), pages 1-11, December.

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