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In vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution

Author

Listed:
  • Wei Chen

    (University of California)

  • Ryan G. Natan

    (University of California)

  • Yuhan Yang

    (University of California)

  • Shih-Wei Chou

    (University of California)

  • Qinrong Zhang

    (University of California)

  • Ehud Y. Isacoff

    (University of California
    Helen Wills Neuroscience Institute, University of California
    Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory)

  • Na Ji

    (University of California
    University of California
    Helen Wills Neuroscience Institute, University of California
    Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory)

Abstract

Studying neuronal activity at synapses requires high spatiotemporal resolution. For high spatial resolution in vivo imaging at depth, adaptive optics (AO) is required to correct sample-induced aberrations. To improve temporal resolution, Bessel focus has been combined with two-photon fluorescence microscopy (2PFM) for fast volumetric imaging at subcellular lateral resolution. To achieve both high-spatial and high-temporal resolution at depth, we develop an efficient AO method that corrects the distorted wavefront of Bessel focus at the objective focal plane and recovers diffraction-limited imaging performance. Applying AO Bessel focus scanning 2PFM to volumetric imaging of zebrafish larval and mouse brains down to 500 µm depth, we demonstrate substantial improvements in the sensitivity and resolution of structural and functional measurements of synapses in vivo. This enables volumetric measurements of synaptic calcium and glutamate activity at high accuracy, including the simultaneous recording of glutamate activity of apical and basal dendritic spines in the mouse cortex.

Suggested Citation

  • Wei Chen & Ryan G. Natan & Yuhan Yang & Shih-Wei Chou & Qinrong Zhang & Ehud Y. Isacoff & Na Ji, 2021. "In vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26965-7
    DOI: 10.1038/s41467-021-26965-7
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    References listed on IDEAS

    as
    1. Kai Wang & Wenzhi Sun & Christopher T. Richie & Brandon K. Harvey & Eric Betzig & Na Ji, 2015. "Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue," Nature Communications, Nature, vol. 6(1), pages 1-6, November.
    2. Karel Svoboda & Winfried Denk & David Kleinfeld & David W. Tank, 1997. "In vivo dendritic calcium dynamics in neocortical pyramidal neurons," Nature, Nature, vol. 385(6612), pages 161-165, January.
    3. Jiyoung Park & Athanasia Papoutsi & Ryan T. Ash & Miguel A. Marin & Panayiota Poirazi & Stelios M. Smirnakis, 2019. "Contribution of apical and basal dendrites to orientation encoding in mouse V1 L2/3 pyramidal neurons," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
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