IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-26965-7.html
   My bibliography  Save this article

In vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution

Author

Listed:
  • Wei Chen

    (University of California)

  • Ryan G. Natan

    (University of California)

  • Yuhan Yang

    (University of California)

  • Shih-Wei Chou

    (University of California)

  • Qinrong Zhang

    (University of California)

  • Ehud Y. Isacoff

    (University of California
    Helen Wills Neuroscience Institute, University of California
    Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory)

  • Na Ji

    (University of California
    University of California
    Helen Wills Neuroscience Institute, University of California
    Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory)

Abstract

Studying neuronal activity at synapses requires high spatiotemporal resolution. For high spatial resolution in vivo imaging at depth, adaptive optics (AO) is required to correct sample-induced aberrations. To improve temporal resolution, Bessel focus has been combined with two-photon fluorescence microscopy (2PFM) for fast volumetric imaging at subcellular lateral resolution. To achieve both high-spatial and high-temporal resolution at depth, we develop an efficient AO method that corrects the distorted wavefront of Bessel focus at the objective focal plane and recovers diffraction-limited imaging performance. Applying AO Bessel focus scanning 2PFM to volumetric imaging of zebrafish larval and mouse brains down to 500 µm depth, we demonstrate substantial improvements in the sensitivity and resolution of structural and functional measurements of synapses in vivo. This enables volumetric measurements of synaptic calcium and glutamate activity at high accuracy, including the simultaneous recording of glutamate activity of apical and basal dendritic spines in the mouse cortex.

Suggested Citation

  • Wei Chen & Ryan G. Natan & Yuhan Yang & Shih-Wei Chou & Qinrong Zhang & Ehud Y. Isacoff & Na Ji, 2021. "In vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26965-7
    DOI: 10.1038/s41467-021-26965-7
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-26965-7
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-26965-7?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Jiyoung Park & Athanasia Papoutsi & Ryan T. Ash & Miguel A. Marin & Panayiota Poirazi & Stelios M. Smirnakis, 2019. "Contribution of apical and basal dendrites to orientation encoding in mouse V1 L2/3 pyramidal neurons," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    2. Kai Wang & Wenzhi Sun & Christopher T. Richie & Brandon K. Harvey & Eric Betzig & Na Ji, 2015. "Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue," Nature Communications, Nature, vol. 6(1), pages 1-6, November.
    3. Karel Svoboda & Winfried Denk & David Kleinfeld & David W. Tank, 1997. "In vivo dendritic calcium dynamics in neocortical pyramidal neurons," Nature, Nature, vol. 385(6612), pages 161-165, January.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Soongyu Yi & Jin Xiang & Ming Zhou & Zhicheng Wu & Lan Yang & Zongfu Yu, 2021. "Angle-based wavefront sensing enabled by the near fields of flat optics," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
    2. Natalia V. Barykina & Erin M. Carey & Olena S. Oliinyk & Axel Nimmerjahn & Vladislav V. Verkhusha, 2024. "Destabilized near-infrared fluorescent nanobodies enable background-free targeting of GFP-based biosensors for imaging and manipulation," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    3. Yuting Li & Zongyue Cheng & Chenmao Wang & Jianian Lin & Hehai Jiang & Meng Cui, 2024. "Geometric transformation adaptive optics (GTAO) for volumetric deep brain imaging through gradient-index lenses," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    4. Romain Daniel Cazé & Mark Humphries & Boris Gutkin, 2013. "Passive Dendrites Enable Single Neurons to Compute Linearly Non-separable Functions," PLOS Computational Biology, Public Library of Science, vol. 9(2), pages 1-15, February.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26965-7. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.