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Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue

Author

Listed:
  • Kai Wang

    (Janelia Research Campus, Howard Hughes Medical Institute)

  • Wenzhi Sun

    (Janelia Research Campus, Howard Hughes Medical Institute)

  • Christopher T. Richie

    (Intramural Research Program, National Institute on Drug Abuse)

  • Brandon K. Harvey

    (Intramural Research Program, National Institute on Drug Abuse)

  • Eric Betzig

    (Janelia Research Campus, Howard Hughes Medical Institute)

  • Na Ji

    (Janelia Research Campus, Howard Hughes Medical Institute)

Abstract

Adaptive optics by direct imaging of the wavefront distortions of a laser-induced guide star has long been used in astronomy, and more recently in microscopy to compensate for aberrations in transparent specimens. Here we extend this approach to tissues that strongly scatter visible light by exploiting the reduced scattering of near-infrared guide stars. The method enables in vivo two-photon morphological and functional imaging down to 700 μm inside the mouse brain.

Suggested Citation

  • Kai Wang & Wenzhi Sun & Christopher T. Richie & Brandon K. Harvey & Eric Betzig & Na Ji, 2015. "Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue," Nature Communications, Nature, vol. 6(1), pages 1-6, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8276
    DOI: 10.1038/ncomms8276
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    Cited by:

    1. Natalia V. Barykina & Erin M. Carey & Olena S. Oliinyk & Axel Nimmerjahn & Vladislav V. Verkhusha, 2024. "Destabilized near-infrared fluorescent nanobodies enable background-free targeting of GFP-based biosensors for imaging and manipulation," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    2. Wei Chen & Ryan G. Natan & Yuhan Yang & Shih-Wei Chou & Qinrong Zhang & Ehud Y. Isacoff & Na Ji, 2021. "In vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    3. Soongyu Yi & Jin Xiang & Ming Zhou & Zhicheng Wu & Lan Yang & Zongfu Yu, 2021. "Angle-based wavefront sensing enabled by the near fields of flat optics," Nature Communications, Nature, vol. 12(1), pages 1-8, December.

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