IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v6y2015i1d10.1038_ncomms8276.html
   My bibliography  Save this article

Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue

Author

Listed:
  • Kai Wang

    (Janelia Research Campus, Howard Hughes Medical Institute)

  • Wenzhi Sun

    (Janelia Research Campus, Howard Hughes Medical Institute)

  • Christopher T. Richie

    (Intramural Research Program, National Institute on Drug Abuse)

  • Brandon K. Harvey

    (Intramural Research Program, National Institute on Drug Abuse)

  • Eric Betzig

    (Janelia Research Campus, Howard Hughes Medical Institute)

  • Na Ji

    (Janelia Research Campus, Howard Hughes Medical Institute)

Abstract

Adaptive optics by direct imaging of the wavefront distortions of a laser-induced guide star has long been used in astronomy, and more recently in microscopy to compensate for aberrations in transparent specimens. Here we extend this approach to tissues that strongly scatter visible light by exploiting the reduced scattering of near-infrared guide stars. The method enables in vivo two-photon morphological and functional imaging down to 700 μm inside the mouse brain.

Suggested Citation

  • Kai Wang & Wenzhi Sun & Christopher T. Richie & Brandon K. Harvey & Eric Betzig & Na Ji, 2015. "Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue," Nature Communications, Nature, vol. 6(1), pages 1-6, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8276
    DOI: 10.1038/ncomms8276
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms8276
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms8276?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Wei Chen & Ryan G. Natan & Yuhan Yang & Shih-Wei Chou & Qinrong Zhang & Ehud Y. Isacoff & Na Ji, 2021. "In vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    2. Soongyu Yi & Jin Xiang & Ming Zhou & Zhicheng Wu & Lan Yang & Zongfu Yu, 2021. "Angle-based wavefront sensing enabled by the near fields of flat optics," Nature Communications, Nature, vol. 12(1), pages 1-8, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8276. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.