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Drosophila ßHeavy-Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil

Author

Listed:
  • Nicole Pogodalla

    (Universität Münster)

  • Holger Kranenburg

    (Universität Münster)

  • Simone Rey

    (Universität Münster)

  • Silke Rodrigues

    (Universität Münster)

  • Albert Cardona

    (Cambridge Biomedical Campus
    University of Cambridge
    HHMI Janelia Research Campus)

  • Christian Klämbt

    (Universität Münster)

Abstract

In the central nervous system (CNS), functional tasks are often allocated to distinct compartments. This is also evident in the Drosophila CNS where synapses and dendrites are clustered in distinct neuropil regions. The neuropil is separated from neuronal cell bodies by ensheathing glia, which as we show using dye injection experiments, contribute to the formation of an internal diffusion barrier. We find that ensheathing glia are polarized with a basolateral plasma membrane rich in phosphatidylinositol-(3,4,5)-triphosphate (PIP3) and the Na+/K+-ATPase Nervana2 (Nrv2) that abuts an extracellular matrix formed at neuropil-cortex interface. The apical plasma membrane is facing the neuropil and is rich in phosphatidylinositol-(4,5)-bisphosphate (PIP2) that is supported by a sub-membranous ßHeavy-Spectrin cytoskeleton. ßHeavy-spectrin mutant larvae affect ensheathing glial cell polarity with delocalized PIP2 and Nrv2 and exhibit an abnormal locomotion which is similarly shown by ensheathing glia ablated larvae. Thus, polarized glia compartmentalizes the brain and is essential for proper nervous system function.

Suggested Citation

  • Nicole Pogodalla & Holger Kranenburg & Simone Rey & Silke Rodrigues & Albert Cardona & Christian Klämbt, 2021. "Drosophila ßHeavy-Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil," Nature Communications, Nature, vol. 12(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26462-x
    DOI: 10.1038/s41467-021-26462-x
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    References listed on IDEAS

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    1. Zhiguo Ma & Tobias Stork & Dwight E. Bergles & Marc R. Freeman, 2016. "Neuromodulators signal through astrocytes to alter neural circuit activity and behaviour," Nature, Nature, vol. 539(7629), pages 428-432, November.
    2. Tomoko Ohyama & Casey M. Schneider-Mizell & Richard D. Fetter & Javier Valdes Aleman & Romain Franconville & Marta Rivera-Alba & Brett D. Mensh & Kristin M. Branson & Julie H. Simpson & James W. Truma, 2015. "A multilevel multimodal circuit enhances action selection in Drosophila," Nature, Nature, vol. 520(7549), pages 633-639, April.
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