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Cell membrane coating integrity affects the internalization mechanism of biomimetic nanoparticles

Author

Listed:
  • Lizhi Liu

    (University of Eastern Finland)

  • Xuan Bai

    (State Key Laboratory of Fluid Power and Mechatronic Systems, Zhejiang University)

  • Maria-Viola Martikainen

    (University of Eastern Finland)

  • Anna Kårlund

    (Institute of Public Health and Clinical Nutrition, University of Eastern Finland)

  • Marjut Roponen

    (University of Eastern Finland)

  • Wujun Xu

    (University of Eastern Finland)

  • Guoqing Hu

    (State Key Laboratory of Fluid Power and Mechatronic Systems, Zhejiang University)

  • Ennio Tasciotti

    (IRCCS San Raffaele Pisana Hospital and San Raffaele University
    Sclavo Pharma)

  • Vesa-Pekka Lehto

    (University of Eastern Finland)

Abstract

Cell membrane coated nanoparticles (NPs) have recently been recognized as attractive nanomedical tools because of their unique properties such as immune escape, long blood circulation time, specific molecular recognition and cell targeting. However, the integrity of the cell membrane coating on NPs, a key metrics related to the quality of these biomimetic-systems and their resulting biomedical function, has remained largely unexplored. Here, we report a fluorescence quenching assay to probe the integrity of cell membrane coating. In contradiction to the common assumption of perfect coating, we uncover that up to 90% of the biomimetic NPs are only partially coated. Using in vitro homologous targeting studies, we demonstrate that partially coated NPs could still be internalized by the target cells. By combining molecular simulations with experimental analysis, we further identify an endocytic entry mechanism for these NPs. We unravel that NPs with a high coating degree (≥50%) enter the cells individually, whereas the NPs with a low coating degree (

Suggested Citation

  • Lizhi Liu & Xuan Bai & Maria-Viola Martikainen & Anna Kårlund & Marjut Roponen & Wujun Xu & Guoqing Hu & Ennio Tasciotti & Vesa-Pekka Lehto, 2021. "Cell membrane coating integrity affects the internalization mechanism of biomimetic nanoparticles," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26052-x
    DOI: 10.1038/s41467-021-26052-x
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    Cited by:

    1. Lin Li & Mengxing Zhang & Jing Li & Tiantian Liu & Qixue Bao & Xi Li & Jiaying Long & Leyao Fu & Zhirong Zhang & Shiqi Huang & Zhenmi Liu & Ling Zhang, 2023. "Cholesterol removal improves performance of a model biomimetic system to co-deliver a photothermal agent and a STING agonist for cancer immunotherapy," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    2. Lizhi Liu & Dingyi Pan & Sheng Chen & Maria-Viola Martikainen & Anna Kårlund & Jing Ke & Herkko Pulkkinen & Hanna Ruhanen & Marjut Roponen & Reijo Käkelä & Wujun Xu & Jie Wang & Vesa-Pekka Lehto, 2022. "Systematic design of cell membrane coating to improve tumor targeting of nanoparticles," Nature Communications, Nature, vol. 13(1), pages 1-15, December.

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