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Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association

Author

Listed:
  • Hossein Mohammad-Beigi

    (Aarhus University
    Aarhus University)

  • Yuya Hayashi

    (Aarhus University)

  • Christina Moeslund Zeuthen

    (Aarhus University
    Aarhus University)

  • Hoda Eskandari

    (Aarhus University
    Aarhus University)

  • Carsten Scavenius

    (Aarhus University)

  • Kristian Juul-Madsen

    (Aarhus University)

  • Thomas Vorup-Jensen

    (Aarhus University)

  • Jan J. Enghild

    (Aarhus University)

  • Duncan S. Sutherland

    (Aarhus University
    Aarhus University)

Abstract

The current understanding of the biological identity that nanoparticles may acquire in a given biological milieu is mostly inferred from the hard component of the protein corona (HC). The composition of soft corona (SC) proteins and their biological relevance have remained elusive due to the lack of analytical separation methods. Here, we identify a set of specific corona proteins with weak interactions at silica and polystyrene nanoparticles by using an in situ click-chemistry reaction. We show that these SC proteins are present also in the HC, but are specifically enriched after the capture, suggesting that the main distinction between HC and SC is the differential binding strength of the same proteins. Interestingly, the weakly interacting proteins are revealed as modulators of nanoparticle-cell association mainly through their dynamic nature. We therefore highlight that weak interactions of proteins at nanoparticles should be considered when evaluating nano-bio interfaces.

Suggested Citation

  • Hossein Mohammad-Beigi & Yuya Hayashi & Christina Moeslund Zeuthen & Hoda Eskandari & Carsten Scavenius & Kristian Juul-Madsen & Thomas Vorup-Jensen & Jan J. Enghild & Duncan S. Sutherland, 2020. "Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association," Nature Communications, Nature, vol. 11(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18237-7
    DOI: 10.1038/s41467-020-18237-7
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    Cited by:

    1. Kristian Juul-Madsen & Peter Parbo & Rola Ismail & Peter L. Ovesen & Vanessa Schmidt & Lasse S. Madsen & Jacob Thyrsted & Sarah Gierl & Mihaela Breum & Agnete Larsen & Morten N. Andersen & Marina Rome, 2024. "Amyloid-β aggregates activate peripheral monocytes in mild cognitive impairment," Nature Communications, Nature, vol. 15(1), pages 1-20, December.
    2. Didar Baimanov & Jing Wang & Jun Zhang & Ke Liu & Yalin Cong & Xiaomeng Shi & Xiaohui Zhang & Yufeng Li & Xiumin Li & Rongrong Qiao & Yuliang Zhao & Yunlong Zhou & Liming Wang & Chunying Chen, 2022. "In situ analysis of nanoparticle soft corona and dynamic evolution," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    3. Shen Han & Richard Costa Marques & Johanna Simon & Anke Kaltbeitzel & Kaloian Koynov & Katharina Landfester & Volker Mailänder & Ingo Lieberwirth, 2023. "Endosomal sorting results in a selective separation of the protein corona from nanoparticles," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    4. Ali Akbar Ashkarran & Hassan Gharibi & Elizabeth Voke & Markita P. Landry & Amir Ata Saei & Morteza Mahmoudi, 2022. "Measurements of heterogeneity in proteomics analysis of the nanoparticle protein corona across core facilities," Nature Communications, Nature, vol. 13(1), pages 1-11, December.

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