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Polydiacetylene-based ultrastrong bioorthogonal Raman probes for targeted live-cell Raman imaging

Author

Listed:
  • Sidan Tian

    (Huazhong University of Science and Technology)

  • Haozheng Li

    (Huazhong University of Science and Technology)

  • Zhong Li

    (Stony Brook University)

  • Huajun Tang

    (Huazhong University of Science and Technology)

  • Mingming Yin

    (Huazhong University of Science and Technology)

  • Yage Chen

    (Huazhong University of Science and Technology)

  • Shun Wang

    (Huazhong University of Science and Technology)

  • Yuting Gao

    (Huazhong University of Science and Technology)

  • Xiangliang Yang

    (Huazhong University of Science and Technology
    Huazhong University of Science and Technology)

  • Fanling Meng

    (Huazhong University of Science and Technology)

  • Joseph W. Lauher

    (Stony Brook University)

  • Ping Wang

    (Huazhong University of Science and Technology
    Huazhong University of Science and Technology)

  • Liang Luo

    (Huazhong University of Science and Technology
    Huazhong University of Science and Technology)

Abstract

Live-cell Raman imaging based on bioorthogonal Raman probes with distinct signals in the cellular Raman-silent region (1800–2800 cm−1) has attracted great interest in recent years. We report here a class of water-soluble and biocompatible polydiacetylenes with intrinsic ultrastrong alkyne Raman signals that locate in this region for organelle-targeting live-cell Raman imaging. Using a host-guest topochemical polymerization strategy, we have synthesized a water-soluble and functionalizable master polydiacetylene, namely poly(deca-4,6-diynedioic acid) (PDDA), which possesses significantly enhanced (up to ~104 fold) alkyne vibration compared to conventional alkyne Raman probes. In addition, PDDA can be used as a general platform for multi-functional ultrastrong Raman probes. We achieve high quality live-cell stimulated Raman scattering imaging on the basis of modified PDDA. The polydiacetylene-based Raman probes represent ultrastrong intrinsic Raman imaging agents in the Raman-silent region (without any Raman enhancer), and the flexible functionalization of this material holds great promise for its potential diverse applications.

Suggested Citation

  • Sidan Tian & Haozheng Li & Zhong Li & Huajun Tang & Mingming Yin & Yage Chen & Shun Wang & Yuting Gao & Xiangliang Yang & Fanling Meng & Joseph W. Lauher & Ping Wang & Liang Luo, 2020. "Polydiacetylene-based ultrastrong bioorthogonal Raman probes for targeted live-cell Raman imaging," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-019-13784-0
    DOI: 10.1038/s41467-019-13784-0
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    Cited by:

    1. Mingming Yin & Xiaoming Liu & Ziqiao Lei & Yuting Gao & Jiacheng Liu & Sidan Tian & Zhiwen Liang & Ye Wang & Fanling Meng & Liang Luo, 2022. "Precisely translating computed tomography diagnosis accuracy into therapeutic intervention by a carbon-iodine conjugated polymer," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    2. Yiyi Zhang & Sidan Tian & Liping Huang & Yanan Li & Yuan Lu & Hongyu Li & Guiping Chen & Fanling Meng & Gang L. Liu & Xiangliang Yang & Jiasheng Tu & Chunmeng Sun & Liang Luo, 2022. "Reactive oxygen species-responsive and Raman-traceable hydrogel combining photodynamic and immune therapy for postsurgical cancer treatment," Nature Communications, Nature, vol. 13(1), pages 1-15, December.

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