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Integrated NMR and cryo-EM atomic-resolution structure determination of a half-megadalton enzyme complex

Author

Listed:
  • Diego F. Gauto

    (Université Grenoble Alpes)

  • Leandro F. Estrozi

    (Université Grenoble Alpes)

  • Charles D. Schwieters

    (National Institutes of Health)

  • Gregory Effantin

    (Université Grenoble Alpes)

  • Pavel Macek

    (Université Grenoble Alpes
    NMR-Bio)

  • Remy Sounier

    (Université Grenoble Alpes
    University of Montpellier)

  • Astrid C. Sivertsen

    (Université Grenoble Alpes)

  • Elena Schmidt

    (Goethe University Frankfurt am Main)

  • Rime Kerfah

    (Université Grenoble Alpes
    NMR-Bio)

  • Guillaume Mas

    (Université Grenoble Alpes
    Biozentrum University of Basel)

  • Jacques-Philippe Colletier

    (Université Grenoble Alpes)

  • Peter Güntert

    (Goethe University Frankfurt am Main
    ETH Zürich
    Tokyo Metropolitan University)

  • Adrien Favier

    (Université Grenoble Alpes)

  • Guy Schoehn

    (Université Grenoble Alpes)

  • Paul Schanda

    (Université Grenoble Alpes)

  • Jerome Boisbouvier

    (Université Grenoble Alpes)

Abstract

Atomic-resolution structure determination is crucial for understanding protein function. Cryo-EM and NMR spectroscopy both provide structural information, but currently cryo-EM does not routinely give access to atomic-level structural data, and, generally, NMR structure determination is restricted to small (

Suggested Citation

  • Diego F. Gauto & Leandro F. Estrozi & Charles D. Schwieters & Gregory Effantin & Pavel Macek & Remy Sounier & Astrid C. Sivertsen & Elena Schmidt & Rime Kerfah & Guillaume Mas & Jacques-Philippe Colle, 2019. "Integrated NMR and cryo-EM atomic-resolution structure determination of a half-megadalton enzyme complex," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10490-9
    DOI: 10.1038/s41467-019-10490-9
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    Cited by:

    1. Chunting Zhang & Changmiao Guo & Ryan W. Russell & Caitlin M. Quinn & Mingyue Li & John C. Williams & Angela M. Gronenborn & Tatyana Polenova, 2022. "Magic-angle-spinning NMR structure of the kinesin-1 motor domain assembled with microtubules reveals the elusive neck linker orientation," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    2. Sara Pfister & Julius Rabl & Thomas Wiegand & Simone Mattei & Alexander A. Malär & Lauriane Lecoq & Stefan Seitz & Ralf Bartenschlager & Anja Böckmann & Michael Nassal & Daniel Boehringer & Beat H. Me, 2023. "Structural conservation of HBV-like capsid proteins over hundreds of millions of years despite the shift from non-enveloped to enveloped life-style," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    3. Diego F. Gauto & Pavel Macek & Duccio Malinverni & Hugo Fraga & Matteo Paloni & Iva Sučec & Audrey Hessel & Juan Pablo Bustamante & Alessandro Barducci & Paul Schanda, 2022. "Functional control of a 0.5 MDa TET aminopeptidase by a flexible loop revealed by MAS NMR," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

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