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Spatiotemporal control of coacervate formation within liposomes

Author

Listed:
  • Siddharth Deshpande

    (Delft University of Technology)

  • Frank Brandenburg

    (Delft University of Technology)

  • Anson Lau

    (Delft University of Technology)

  • Mart G. F. Last

    (Delft University of Technology)

  • Willem Kasper Spoelstra

    (Delft University of Technology)

  • Louis Reese

    (Delft University of Technology)

  • Sreekar Wunnava

    (Delft University of Technology)

  • Marileen Dogterom

    (Delft University of Technology)

  • Cees Dekker

    (Delft University of Technology)

Abstract

Liquid-liquid phase separation (LLPS), especially coacervation, plays a crucial role in cell biology, as it forms numerous membraneless organelles in cells. Coacervates play an indispensable role in regulating intracellular biochemistry, and their dysfunction is associated with several diseases. Understanding of the LLPS dynamics would greatly benefit from controlled in vitro assays that mimic cells. Here, we use a microfluidics-based methodology to form coacervates inside cell-sized (~10 µm) liposomes, allowing control over the dynamics. Protein-pore-mediated permeation of small molecules into liposomes triggers LLPS passively or via active mechanisms like enzymatic polymerization of nucleic acids. We demonstrate sequestration of proteins (FtsZ) and supramolecular assemblies (lipid vesicles), as well as the possibility to host metabolic reactions (β-galactosidase activity) inside coacervates. This coacervate-in-liposome platform provides a versatile tool to understand intracellular phase behavior, and these hybrid systems will allow engineering complex pathways to reconstitute cellular functions and facilitate bottom-up creation of synthetic cells.

Suggested Citation

  • Siddharth Deshpande & Frank Brandenburg & Anson Lau & Mart G. F. Last & Willem Kasper Spoelstra & Louis Reese & Sreekar Wunnava & Marileen Dogterom & Cees Dekker, 2019. "Spatiotemporal control of coacervate formation within liposomes," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
  • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09855-x
    DOI: 10.1038/s41467-019-09855-x
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    Cited by:

    1. Shoupeng Cao & Tsvetomir Ivanov & Julian Heuer & Calum T. J. Ferguson & Katharina Landfester & Lucas Caire da Silva, 2024. "Dipeptide coacervates as artificial membraneless organelles for bioorthogonal catalysis," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    2. Andrea Testa & Mirco Dindo & Aleksander A. Rebane & Babak Nasouri & Robert W. Style & Ramin Golestanian & Eric R. Dufresne & Paola Laurino, 2021. "Sustained enzymatic activity and flow in crowded protein droplets," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
    3. Agustín Mangiarotti & Nannan Chen & Ziliang Zhao & Reinhard Lipowsky & Rumiana Dimova, 2023. "Wetting and complex remodeling of membranes by biomolecular condensates," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    4. Hanjin Seo & Hyomin Lee, 2022. "Spatiotemporal control of signal-driven enzymatic reaction in artificial cell-like polymersomes," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    5. Jiahua Wang & Manzar Abbas & Junyou Wang & Evan Spruijt, 2023. "Selective amide bond formation in redox-active coacervate protocells," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    6. Shang Dai & Zhenming Xie & Binqiang Wang & Rui Ye & Xinwen Ou & Chen Wang & Ning Yu & Cheng Huang & Jie Zhao & Chunhui Cai & Furong Zhang & Damiano Buratto & Taimoor Khan & Yan Qiao & Yuejin Hua & Ruh, 2023. "An inorganic mineral-based protocell with prebiotic radiation fitness," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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