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A Sustainable Approach to In Vitro Propagation and Conservation of Salvia dominica L.: A Wild Medicinal Plant from Jordan

Author

Listed:
  • Tamara S. Al-Qudah

    (Hamdi Mango Center for Scientific Research (HMCSR), University of Jordan, Amman 11942, Jordan)

  • Rida A. Shibli

    (Department of Agricultural Biotechnology and Genetic Engineering, Faculty of Agriculture Technology, Al-Ahliyya Amman University, Amman 19328, Jordan
    Department of Horticulture and Crop Sciences, Faculty of Agriculture, University of Jordan, Amman 11942, Jordan)

  • Ahmad Zatimeh

    (Department of Applied Sciences, Huson College, Al Balqa-Applied University, Irbid 19117, Jordan)

  • Reham W. Tahtamouni

    (Department of Applied and Social Sciences, Princess Alia University College, Al-Balqa Applied University, Amman 11191, Jordan)

  • Firas Al-Zyoud

    (Department of Plant Protection and IPM, Faculty of Agriculture, Mutah University, Karak 61710, Jordan)

Abstract

Salvia dominica L. is an important wild medicinal plant that grows in Jordan and neighboring countries, and this plant has been suffering from many threats in its wild environment. Therefore, this research aims to establish a comprehensive and sustainable approach via an in vitro propagation and conservation system for the S. dominica L. plant. Axillary buds were used to initiate the in vitro culture on Murashige and Skoog MS media supplemented with 0.5 mg L −1 of GA 3 . In vitro shoot proliferation and rooting were experimented on with different concentrations of cytokinins and auxins, respectively. Calli were induced in the dark on excised leaf discs (0.5 cm in diameter), and multiplication was experimented on with different growth regulators. Cryopreservation experiments were applied on the callused segments under different growth conditions via the vitrification technique. A full protocol was achieved for shoot proliferation with 6.3 shoots/explant using 1.2 mg L −1 of thidiazuron (TDZ), while rooting was achieved at 1.5 mg L −1 of NAA with 6.6 functional roots/explant. Acclimatization was completely successful for the rooted plants. The highest callus production with 5.81 g/calli was achieved using 1.5 mg L −1 of benzylaminopurine (BAP). Cryopreservation of the S. dominica calli was successfully achieved when a pure plant vitrification solution (PVS2) was used to dehydrate the calli for 20 min after immersion in the loading solution for 20 min with a 76.6% regrowth percentage. The loading and the plant vitrification solution type and duration were the most critical points in the regrowth of the cryopreserved calli. In conclusion, a successful protocol was set up for the in vitro propagation and conservation of S. dominica calli. This study has prompted us to perform further studies on sustainable in vitro production and conservation of critically endangered medicinal plants to implement a green environment protecting against surrounding threats.

Suggested Citation

  • Tamara S. Al-Qudah & Rida A. Shibli & Ahmad Zatimeh & Reham W. Tahtamouni & Firas Al-Zyoud, 2023. "A Sustainable Approach to In Vitro Propagation and Conservation of Salvia dominica L.: A Wild Medicinal Plant from Jordan," Sustainability, MDPI, vol. 15(19), pages 1-13, September.
    • Handle: RePEc:gam:jsusta:v:15:y:2023:i:19:p:14218-:d:1247990
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    References listed on IDEAS

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