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Environmental DNA and Specific Primers for Detecting the Invasive Species Ectopleura crocea (Hydrozoa: Anthoathecata) in Seawater Samples

Author

Listed:
  • Philjae Kim

    (Research & Development Division, National Science Museum, Daejeon 34143, Korea)

  • Tae Joong Yoon

    (Institute of Marine Life Resources, Sahmyook University, Seoul 01795, Korea)

  • Sook Shin

    (Institute of Marine Life Resources, Sahmyook University, Seoul 01795, Korea
    Department of Animal Biotechnology & Resource, Sahmyook University, Seoul 01795, Korea)

Abstract

In marine environments, environmental DNA (eDNA) can be effectively detected and possibly quantified when combined with molecular techniques, as demonstrated by several recent studies. In this study, we developed a species-specific primer set and a probe to detect the distribution and biomass of an invasive hydrozoan in South Korea, Ectopleura crocea . These molecular markers were designed to amplify a 187 bp region based on mitochondrial cytochrome c oxidase subunit I (COI) of E. crocea and were tested on seawater samples from 35 Korean harbors in 2017. Of the 35 sites we investigated, only nine harbors returned positive detections when using traditional survey methods, while surveys based on the use of eDNA techniques detected E. crocea DNA in all seawater samples. These results suggest that eDNA surveys based on molecular techniques are more effective at identifying species distribution and estimating biomass than traditional surveys based on visual assessment of morphology.

Suggested Citation

  • Philjae Kim & Tae Joong Yoon & Sook Shin, 2020. "Environmental DNA and Specific Primers for Detecting the Invasive Species Ectopleura crocea (Hydrozoa: Anthoathecata) in Seawater Samples," Sustainability, MDPI, vol. 12(6), pages 1-17, March.
    • Handle: RePEc:gam:jsusta:v:12:y:2020:i:6:p:2360-:d:333857
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    References listed on IDEAS

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    1. Bax, Nicholas & Williamson, Angela & Aguero, Max & Gonzalez, Exequiel & Geeves, Warren, 2003. "Marine invasive alien species: a threat to global biodiversity," Marine Policy, Elsevier, vol. 27(4), pages 313-323, July.
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