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Ultramicrobacteria from Nitrate- and Radionuclide-Contaminated Groundwater

Author

Listed:
  • Tamara Nazina

    (Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia
    V.I. Vernadsky Institute of Geochemistry and Analytical Chemistry of Russian Academy of Sciences, 119071 Moscow, Russia)

  • Tamara Babich

    (Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia)

  • Nadezhda Kostryukova

    (Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia)

  • Diyana Sokolova

    (Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia)

  • Ruslan Abdullin

    (Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia)

  • Tatyana Tourova

    (Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia)

  • Vitaly Kadnikov

    (Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia)

  • Andrey Mardanov

    (Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia)

  • Nikolai Ravin

    (Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia)

  • Denis Grouzdev

    (Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia)

  • Andrey Poltaraus

    (Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119071 Moscow, Russia)

  • Stepan Kalmykov

    (Chemical Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia)

  • Alexey Safonov

    (Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 119071 Moscow, Russia)

  • Elena Zakharova

    (Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 119071 Moscow, Russia)

  • Alexander Novikov

    (V.I. Vernadsky Institute of Geochemistry and Analytical Chemistry of Russian Academy of Sciences, 119071 Moscow, Russia)

  • Kenji Kato

    (Faculty of Science, Department of Geosciences, Shizuoka University, 422-8529 Shizuoka, Japan)

Abstract

The goal of the present work was to investigate the physicochemical and radiochemical conditions and the microbial diversity in groundwater collected near the Lake Karachai (Russia), which was formerly used for the disposal of liquid radioactive waste, to isolate the dominant bacteria, and to determine their taxonomy and the physiological characteristics responsible for their adaptation to this environment. Groundwater samples contained high concentrations of acetate, oxalate, nitrate, and sulfate, as well as radionuclides. High-throughput sequencing and analysis of the clone libraries revealed lower microbial diversity in the most strongly contaminated groundwater and a predominance of bacteria of the genera Polynucleobacter , Pusillimonas , Candidatus Pelagibacter , and of the candidate phylum Parcubacteria ; these groups include species with an ultra small cell size. Archaeal sequences in the libraries belonged to ammonium oxidizers of the phylum Thaumarchaeota and methanogens of the phylum Euryarchaeota . Pure cultures of obligate and facultative ultramicrobacteria belonging to the genera Chryseobacterium , Microbacterium , Salinibacterium , Pusillimonas , Roseomonas , and Janibacter were isolated from water samples. In genomes of Pusillimonas and Roseomonas strains the genes associated with nitrate reduction, resistance to heavy metals and metalloids were revealed. Several isolates are able to participate in the geochemical process of nitrate conversion to N 2 using acetate; this results in decreasing redox potential, which in turn may stimulate radionuclide reduction and decrease radionuclide migration in groundwater.

Suggested Citation

  • Tamara Nazina & Tamara Babich & Nadezhda Kostryukova & Diyana Sokolova & Ruslan Abdullin & Tatyana Tourova & Vitaly Kadnikov & Andrey Mardanov & Nikolai Ravin & Denis Grouzdev & Andrey Poltaraus & Ste, 2020. "Ultramicrobacteria from Nitrate- and Radionuclide-Contaminated Groundwater," Sustainability, MDPI, vol. 12(3), pages 1-27, February.
  • Handle: RePEc:gam:jsusta:v:12:y:2020:i:3:p:1239-:d:318217
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    References listed on IDEAS

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    1. Christian Rinke & Patrick Schwientek & Alexander Sczyrba & Natalia N. Ivanova & Iain J. Anderson & Jan-Fang Cheng & Aaron Darling & Stephanie Malfatti & Brandon K. Swan & Esther A. Gies & Jeremy A. Do, 2013. "Insights into the phylogeny and coding potential of microbial dark matter," Nature, Nature, vol. 499(7459), pages 431-437, July.
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