IDEAS home Printed from https://ideas.repec.org/a/gam/jsusta/v12y2020i12p5156-d375729.html
   My bibliography  Save this article

In Vitro Study of Butyric Acid Deodorization Potential by Indigenously Constructed Bacterial Consortia and Pure Cultures from Pit Latrine Fecal Sludge

Author

Listed:
  • John Bright Joseph Njalam’mano

    (Water Utilisation and Environmental Engineering Division, Department of Chemical Engineering, University of Pretoria, Private Bag X20, Hatfield, Pretoria 0028, South Africa)

  • Evans Martin Nkhalambayausi Chirwa

    (Water Utilisation and Environmental Engineering Division, Department of Chemical Engineering, University of Pretoria, Private Bag X20, Hatfield, Pretoria 0028, South Africa)

  • Refilwe Lesego Seabi

    (Water Utilisation and Environmental Engineering Division, Department of Chemical Engineering, University of Pretoria, Private Bag X20, Hatfield, Pretoria 0028, South Africa)

Abstract

The present study aims at developing an efficient bacterial consortium to biodegrade butyric acid, one of the odor-causing compounds that contribute significantly to pit latrine malodors. Six bacterial strains isolated from pit latrine fecal sludge were selected for the study. Nineteen bacterial consortia of different combinations were artificially constructed. The individual bacterial strains and bacterial consortia were compared by culturing in mineral salt medium supplemented with 1000 mg/L butyric acid as a sole carbon and energy source at pH 7, 30 °C, and 110 rpm under aerobic growth conditions. A co-culture of Serratia marcescens and Bacillus cereus was an effective bacterial consortium compared to individual component bacterial strains and other bacterial consortia, in which 1000 mg/L butyric acid was completely degraded within 16 h of incubation. A temperature of 30 °C and pH 7 were found to be optimum for the maximum degradation for both S. marcescens and B. cereus . The inoculation sizes of 2.0 and 2.5 were optimal for the maximum degradation for B. cereus and S. marcescens , respectively. The study provides insights that will be of substantial help in the development of effective biological treatment technologies for pit latrine odor to change the pit latrine user community’s and would be users’ perception of pit latrines.

Suggested Citation

  • John Bright Joseph Njalam’mano & Evans Martin Nkhalambayausi Chirwa & Refilwe Lesego Seabi, 2020. "In Vitro Study of Butyric Acid Deodorization Potential by Indigenously Constructed Bacterial Consortia and Pure Cultures from Pit Latrine Fecal Sludge," Sustainability, MDPI, vol. 12(12), pages 1-18, June.
    • Handle: RePEc:gam:jsusta:v:12:y:2020:i:12:p:5156-:d:375729
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2071-1050/12/12/5156/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2071-1050/12/12/5156/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Meredith E Stocks & Stephanie Ogden & Danny Haddad & David G Addiss & Courtney McGuire & Matthew C Freeman, 2014. "Effect of Water, Sanitation, and Hygiene on the Prevention of Trachoma: A Systematic Review and Meta-Analysis," PLOS Medicine, Public Library of Science, vol. 11(2), pages 1-29, February.
    2. Jamie Bartram & Sandy Cairncross, 2010. "Hygiene, Sanitation, and Water: Forgotten Foundations of Health," Working Papers id:3325, eSocialSciences.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Rachel L Pullan & Matthew C Freeman & Peter W Gething & Simon J Brooker, 2014. "Geographical Inequalities in Use of Improved Drinking Water Supply and Sanitation across Sub-Saharan Africa: Mapping and Spatial Analysis of Cross-sectional Survey Data," PLOS Medicine, Public Library of Science, vol. 11(4), pages 1-17, April.
    2. Aderita Sena & Kristie Ebi, 2020. "When Land Is Under Pressure Health Is Under Stress," IJERPH, MDPI, vol. 18(1), pages 1-24, December.
    3. The PLOS Medicine Editors, 2014. "PLOS Medicine and Water, Sanitation, and Hygiene: A Committed Relationship," PLOS Medicine, Public Library of Science, vol. 11(3), pages 1-2, March.
    4. Simon Meunier & Dale T. Manning & Loic Queval & Judith A. Cherni & Philippe Dessante & Daniel Zimmerle, 2019. "Determinants of the marginal willingness to pay for improved domestic water and irrigation in partially electrified Rwandan villages," Post-Print hal-02179229, HAL.
    5. Durrans, Sophie & Last, Anna & Boiro, Hamadou & Goncalves, Adriana & Mabey, David & Greenland, Katie, 2019. "“Moving like birds”: A qualitative study of population mobility and health implications in the Bijagós Islands, Guinea Bissau," Social Science & Medicine, Elsevier, vol. 230(C), pages 204-213.
    6. Sifat Rabbi & Nepal C Dey, 2013. "Exploring the gap between hand washing knowledge and practices in Bangladesh: a cross-sectional comparative study," Working Papers id:5257, eSocialSciences.
    7. Mallick, Rahul & Mandal, Salim & Chouhan, Pradip, 2020. "Impact of sanitation and clean drinking water on the prevalence of diarrhea among the under-five children in India," Children and Youth Services Review, Elsevier, vol. 118(C).
    8. Tracy Morse & Kondwani Chidziwisano & Elizabeth Tilley & Rossanie Malolo & Save Kumwenda & Janelisa Musaya & Sandy Cairncross, 2019. "Developing a Contextually Appropriate Integrated Hygiene Intervention to Achieve Sustained Reductions in Diarrheal Diseases," Sustainability, MDPI, vol. 11(17), pages 1-17, August.
    9. Emmy De Buck & Hans Van Remoortel & Karin Hannes & Thashlin Govender & Selvan Naidoo & Bert Avau & Axel Vande Veegaete & Alfred Musekiwa & Vittoria Lutje & Margaret Cargo & Hans‐Joachim Mosler & Phili, 2017. "Approaches to promote handwashing and sanitation behaviour change in low‐ and middle‐income countries: a mixed method systematic review," Campbell Systematic Reviews, John Wiley & Sons, vol. 13(1), pages 1-447.
    10. Rocío Florez, 2011. "Integrating Hygiene Promotion Into World Bank Projects : Experiences from Colombia and Peru," World Bank Publications - Reports 17346, The World Bank Group.
    11. Marjorie Chinen & Thomas de Hoop & María Balarin & Lorena Alcázar, 2016. "PROTOCOL: Vocational and business training to increase women's participation in higher skilled occupations in low‐ and middle‐income countries: protocol for a systematic review," Campbell Systematic Reviews, John Wiley & Sons, vol. 12(1), pages 1-46.
    12. Mirko S. Winkler & Samuel Fuhrimann & Phuc Pham-Duc & Guéladio Cissé & Jürg Utzinger & Hung Nguyen-Viet, 2017. "Assessing potential health impacts of waste recovery and reuse business models in Hanoi, Vietnam," International Journal of Public Health, Springer;Swiss School of Public Health (SSPH+), vol. 62(1), pages 7-16, February.
    13. McMichael, Celia & Robinson, Priscilla, 2016. "Drivers of sustained hygiene behaviour change: A case study from mid-western Nepal," Social Science & Medicine, Elsevier, vol. 163(C), pages 28-36.
    14. Thandi Kapwata & Angela Mathee & Wouter Jacobus Le Roux & Caradee Yael Wright, 2018. "Diarrhoeal Disease in Relation to Possible Household Risk Factors in South African Villages," IJERPH, MDPI, vol. 15(8), pages 1-12, August.
    15. Joseph Kangmennaang & Elijah Bisung & Susan J. Elliott, 2020. "‘We Are Drinking Diseases’: Perception of Water Insecurity and Emotional Distress in Urban Slums in Accra, Ghana," IJERPH, MDPI, vol. 17(3), pages 1-17, January.
    16. Parfait K. Kouamé & Kouassi Dongo & Hung Nguyen-Viet & Christian Zurbrügg & Christoph Lüthi & Jan Hattendorf & Jürg Utzinger & Jean Biémi & Bassirou Bonfoh, 2014. "Ecohealth Approach to Urban Waste Management: Exposure to Environmental Pollutants and Health Risks in Yamoussoukro, Côte d’Ivoire," IJERPH, MDPI, vol. 11(10), pages 1-18, October.
    17. Weiyu Yu & Nicola A Wardrop & Robert E S Bain & Victor Alegana & Laura J Graham & Jim A Wright, 2019. "Mapping access to domestic water supplies from incomplete data in developing countries: An illustrative assessment for Kenya," PLOS ONE, Public Library of Science, vol. 14(5), pages 1-19, May.
    18. World Bank, 2021. "Niger Investing in Human Capital, Agricultural Productivity, and Social Protection for Faster Poverty Reduction," World Bank Publications - Reports 36590, The World Bank Group.
    19. Deres Asnake & Metadel Adane, 2020. "Household latrine utilization and associated factors in semi-urban areas of northeastern Ethiopia," PLOS ONE, Public Library of Science, vol. 15(11), pages 1-15, November.
    20. Ibukun Fatunmibi, 2023. "A Research Proposal to Address the Gap in Knowledge Affiliated to the Sustainable Development Goal Number Six (SDG-6) Interconnection with Public Health and to Proffer Strategy for Dissemination and I," International Journal of Research and Innovation in Social Science, International Journal of Research and Innovation in Social Science (IJRISS), vol. 7(12), pages 1473-1485, December.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jsusta:v:12:y:2020:i:12:p:5156-:d:375729. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.