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Three Experimental Common High-Risk Procedures: Emission Characteristics Identification and Source Intensity Estimation in Biosafety Laboratory

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  • Zhijian Liu

    (Department of Power Engineering, North China Electric Power University, Baoding 071003, China)

  • Jiabin Lv

    (Department of Power Engineering, North China Electric Power University, Baoding 071003, China)

  • Zheng Zhang

    (Department of Power Engineering, North China Electric Power University, Baoding 071003, China)

  • Juntao Ma

    (Department of Power Engineering, North China Electric Power University, Baoding 071003, China)

  • Yangfan Song

    (Department of Power Engineering, North China Electric Power University, Baoding 071003, China)

  • Minnan Wu

    (Department of Power Engineering, North China Electric Power University, Baoding 071003, China)

  • Guoqing Cao

    (Institute of Building Environment and Energy, China Academy of Building Research, Beijing 100013, China)

  • Jiacheng Guo

    (Department of Power Engineering, North China Electric Power University, Baoding 071003, China)

Abstract

Biosafety laboratory is an important place to study high-risk microbes. In biosafety laboratories, with the outbreak of infectious diseases such as COVID-19, experimental activities have become increasingly frequent, and the risk of exposure to bioaerosols has increased. To explore the exposure risk of biosafety laboratories, the intensity and emission characteristics of laboratory risk factors were investigated. In this study, high-risk microbe samples were substituted with Serratia marcescens as the model bacteria. The resulting concentration and particle size segregation of the bioaerosol produced by three experimental procedures (spill, injection, and sample drop) were monitored, and the emission sources’ intensity were quantitatively analyzed. The results showed that the aerosol concentration produced by injection and sample drop was 10 3 CFU/m 3 , and that by sample spill was 10 2 CFU/m 3 . The particle size of bioaerosol is mainly segregated in the range of 3.3–4.7 μ m. There are significant differences in the influence of risk factors on source intensity. The intensity of sample spill, injection, and sample drop source is 3.6 CFU/s, 78.2 CFU/s, and 664 CFU/s. This study could provide suggestions for risk assessment of experimental operation procedures and experimental personnel protection.

Suggested Citation

  • Zhijian Liu & Jiabin Lv & Zheng Zhang & Juntao Ma & Yangfan Song & Minnan Wu & Guoqing Cao & Jiacheng Guo, 2023. "Three Experimental Common High-Risk Procedures: Emission Characteristics Identification and Source Intensity Estimation in Biosafety Laboratory," IJERPH, MDPI, vol. 20(5), pages 1-11, March.
    • Handle: RePEc:gam:jijerp:v:20:y:2023:i:5:p:4479-:d:1086349
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    References listed on IDEAS

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    1. Chen, Hao & Wang, Yu & Zuo, Mingsheng & Zhang, Chao & Jia, Ninghong & Liu, Xiliang & Yang, Shenglai, 2022. "A new prediction model of CO2 diffusion coefficient in crude oil under reservoir conditions based on BP neural network," Energy, Elsevier, vol. 239(PC).
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