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Multilevel Toxicity Evaluations of Polyethylene Microplastics in Zebrafish ( Danio rerio )

Author

Listed:
  • Ingrid de Souza Freire

    (Departamento de Genética e Morfologia, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília 70910-900, Brazil)

  • Maria Luiza Fascineli

    (Departamento de Genética e Morfologia, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília 70910-900, Brazil)

  • Tathyana Benetis Piau

    (Departamento de Genética e Morfologia, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília 70910-900, Brazil)

  • Bruno Fiorelini Pereira

    (Departmento de Ciências Biológicas, Universidade Federal de São Paulo—UNIFESP, São Paulo 05468-901, Brazil)

  • Cesar Koppe Grisolia

    (Departamento de Genética e Morfologia, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília 70910-900, Brazil)

Abstract

Microplastics in freshwater environments pose a serious threat to living beings. Polyethylene microplastics (PE-MP) are the type most used around the world as microbeads in personal care products, and they have been found in aquatic organisms. The behavior and toxicity of fluorescent PE-MP spheres with an average diameter of 58.9 μm were studied in adult, juvenile and embryo zebrafish ( Danio rerio ). The adults were studied for genotoxicity, cytotoxicity, histology and biochemical markers. Juveniles underwent a follow-up in the gastrointestinal (GI) tract with histologic observations, and embryos were studied for embryotoxicity with the FET-test. In adults, micronucleus test and comet assays found neither genotoxicity after acute exposure for 96 h at concentrations of 0.0, 12.5, 50 and 100 mg.L −1 , nor cytotoxicity through the nuclear abnormalities test. Acetylcholinesterase (AChE), Glutathione-S-Transferase (GST) and Lactate Dehydrogenase (LDH) activities were measured in adults exposed for 96 h. The AChE and GST activities were significantly changed, while no changes occurred for LDH. In conclusion, these PE-MP spheres did not cause serious toxic effects in zebrafish because there was no internalization. The observed biochemical changes in AChE and GST may be associated with GI microbiological dysbiosis, previously reported. The PE-MP spheres in the intestine of juveniles remained present for 12–15 days on average after the post-exposure clearance study, showing a slow depuration. The histological analysis, in adults, found no internalization of these microbeads, with complete depuration. The PE-MP spheres did not cross the chorion barrier, showing no embryotoxic effects after exposures at 0.0, 6.25, 12.5, 50.0 or 100.0 mg.L −1 for 96 h.

Suggested Citation

  • Ingrid de Souza Freire & Maria Luiza Fascineli & Tathyana Benetis Piau & Bruno Fiorelini Pereira & Cesar Koppe Grisolia, 2023. "Multilevel Toxicity Evaluations of Polyethylene Microplastics in Zebrafish ( Danio rerio )," IJERPH, MDPI, vol. 20(4), pages 1-11, February.
    • Handle: RePEc:gam:jijerp:v:20:y:2023:i:4:p:3617-:d:1072529
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    References listed on IDEAS

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    1. Kerstin Howe & Matthew D. Clark & Carlos F. Torroja & James Torrance & Camille Berthelot & Matthieu Muffato & John E. Collins & Sean Humphray & Karen McLaren & Lucy Matthews & Stuart McLaren & Ian Sea, 2013. "The zebrafish reference genome sequence and its relationship to the human genome," Nature, Nature, vol. 496(7446), pages 498-503, April.
    2. Anjar Tri Wibowo & Husna Nugrahapraja & Ruri Agung Wahyuono & Izzatul Islami & Muhammad Husain Haekal & Yasri Fardiansyah & Pramudya Wisnu Wicaksono Sugiyo & Yohanes Kartjito Putro & Faiza Nur Fauzia , 2021. "Microplastic Contamination in the Human Gastrointestinal Tract and Daily Consumables Associated with an Indonesian Farming Community," Sustainability, MDPI, vol. 13(22), pages 1-10, November.
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