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Effect of Water-Pipe Smoking on the Normal Development of Zebrafish

Author

Listed:
  • Zain Zaki Zakaria

    (Department of Biomedical Sciences, College of Health Sciences, QU Health, Qatar University, Doha P.O. Box 2713, Qatar
    Biomedical Research Center, Qatar University, Doha P.O. Box 2713, Qatar)

  • Shaima Ahmad Aladwi

    (Department of Biomedical Sciences, College of Health Sciences, QU Health, Qatar University, Doha P.O. Box 2713, Qatar)

  • Fatiha Benslimane

    (Biomedical Research Center, Qatar University, Doha P.O. Box 2713, Qatar)

  • Enas S. Al-Absi

    (Department of Biomedical Sciences, College of Health Sciences, QU Health, Qatar University, Doha P.O. Box 2713, Qatar
    Biomedical Research Center, Qatar University, Doha P.O. Box 2713, Qatar)

  • Mashael Al-Shafai

    (Department of Biomedical Sciences, College of Health Sciences, QU Health, Qatar University, Doha P.O. Box 2713, Qatar
    Biomedical and Pharmaceutical Research Unit, QU Health, Qatar University, Doha P.O. Box 2713, Qatar)

  • Huseyin C. Yalcin

    (Biomedical Research Center, Qatar University, Doha P.O. Box 2713, Qatar)

  • Ashraf Khalil

    (Biomedical and Pharmaceutical Research Unit, QU Health, Qatar University, Doha P.O. Box 2713, Qatar
    College of Pharmacy, QU Health, Qatar University, Doha P.O. Box 2713, Qatar)

  • Ala-Eddin Al Moustafa

    (Biomedical Research Center, Qatar University, Doha P.O. Box 2713, Qatar
    Biomedical and Pharmaceutical Research Unit, QU Health, Qatar University, Doha P.O. Box 2713, Qatar
    College of Medicine, QU Health, Qatar University, Doha P.O. Box 2713, Qatar)

  • Maha Al-Asmakh

    (Department of Biomedical Sciences, College of Health Sciences, QU Health, Qatar University, Doha P.O. Box 2713, Qatar
    Biomedical Research Center, Qatar University, Doha P.O. Box 2713, Qatar
    Biomedical and Pharmaceutical Research Unit, QU Health, Qatar University, Doha P.O. Box 2713, Qatar)

Abstract

Background: Among all types of tobacco consumption, Water-Pipe Smoking (WPS) is the most widely used in the Middle East and second-most in several other countries. The effect of WPS on normal development is not yet fully understood, thus the aim of this study is to explore the acute toxicity effects of WPS extract on zebrafish larvae. Methods: In this study, we compared the effects of WPS smoke condensates at concentrations varying from 50 to 200 µg/mL on developmental, cardiac, and behavioural (neurotoxicity) functions. Gene expression patterns of cardiac biomarkers were also evaluated by RT-qPCR. Results: Exposing zebrafish embryos to 50, 100, 150 and 200 µg/mL WPS for three days did not affect the normal morphology of Zebrafish embryos, as the tail flicking, behavioural and locomotion assays did not show any change. However, WPS deregulated cardiac markers including atrial natriuretic peptide (ANP/NPPA) and brain natriuretic peptide (BNP/NPPB). Furthermore, it induced apoptosis in a dose-dependent manner. Conclusion: Our data demonstrate that WPS can significantly affect specific cardiac parameters during the normal development of zebrafish. Further investigations are necessary to elucidate the pathogenic outcome of WPS on different aspects of human life, including pregnancy.

Suggested Citation

  • Zain Zaki Zakaria & Shaima Ahmad Aladwi & Fatiha Benslimane & Enas S. Al-Absi & Mashael Al-Shafai & Huseyin C. Yalcin & Ashraf Khalil & Ala-Eddin Al Moustafa & Maha Al-Asmakh, 2021. "Effect of Water-Pipe Smoking on the Normal Development of Zebrafish," IJERPH, MDPI, vol. 18(21), pages 1-13, November.
    • Handle: RePEc:gam:jijerp:v:18:y:2021:i:21:p:11659-:d:673552
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    References listed on IDEAS

    as
    1. Kerstin Howe & Matthew D. Clark & Carlos F. Torroja & James Torrance & Camille Berthelot & Matthieu Muffato & John E. Collins & Sean Humphray & Karen McLaren & Lucy Matthews & Stuart McLaren & Ian Sea, 2013. "The zebrafish reference genome sequence and its relationship to the human genome," Nature, Nature, vol. 496(7446), pages 498-503, April.
    2. IfeanyiChukwu O. Onor & Daniel L. Stirling & Shandrika R. Williams & Daniel Bediako & Amne Borghol & Martha B. Harris & Tiernisha B. Darensburg & Sharde D. Clay & Samuel C. Okpechi & Daniel F. Sarpong, 2017. "Clinical Effects of Cigarette Smoking: Epidemiologic Impact and Review of Pharmacotherapy Options," IJERPH, MDPI, vol. 14(10), pages 1-16, September.
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