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Influence of Carbon Sources on Biomass and Biomolecule Accumulation in Picochlorum sp. Cultured under the Mixotrophic Condition

Author

Listed:
  • Rahul Kumar Goswami

    (Bioprocess and Bioenergy Laboratory, Department of Microbiology, Central University of Rajasthan, Kishangarh 305817, India)

  • Sanjeet Mehariya

    (Department of Engineering, University of Campania “Luigi Vanvitelli”, Real Casa dell’Annunziata, Via Roma 29, 81031 Aversa, Italy)

  • Obulisamy Parthiba Karthikeyan

    (Department of Engineering Technology, College of Technology, University of Houston, Houston, TX 77400, USA
    Institute of Bioresource and Agriculture, Hong Kong Baptist University, Hong Kong, China
    Department of Civil and Environmental Engineering, South Dakota School of Mines and Technology, Rapid City, SD 57701, USA)

  • Pradeep Verma

    (Bioprocess and Bioenergy Laboratory, Department of Microbiology, Central University of Rajasthan, Kishangarh 305817, India)

Abstract

The major downfalls of the microalgal biorefinery are low volume of high value product accumulation, low biomass productivity and high cultivation costs. Here, we aimed to improve the biomass productivity of the industrially relevant Picochlorum sp. BDUG 100241 strain. The growth of Picochlorum sp. BDUG 100241 was investigated under different cultivations conditions, including photoautotrophic (with light), mixotrophic (1% glucose, with light) and heterotrophic (1% glucose, without light). Among them, Picochlorum sp. BDUG100241 showed the highest growth in the mixotrophic condition. Under different (1%) carbon sources’ supplementation, including glucose, sodium acetate, glycerol, citric acid and methanol, Picochlorum sp. BDUG100241 growth was tested. Among them, sodium acetate was found to be most suitable carbon source for Picochlorum sp. BDUG 100241 growth, biomass (1.67 ± 0.18 g/L) and biomolecule productivity. From the different concentrations of sodium acetate (0, 2.5, 5.0, 7.5 and 10 g/L) tested, the maximum biomass production of 2.40 ± 0.20 g/L with the biomass productivity of 95 ± 5.00 mg/L/d was measured from 7.5 g/L in sodium acetate. The highest total lipid (53.50 ± 1.70%) and total carotenoids (0.75 ± 0.01 µg/mL) contents were observed at the concentration of 7.5 g/L and 5.0 g/L of sodium acetate as a carbon source, respectively. In conclusion, the mixotrophic growth condition containing 7.5 g/L of sodium acetate showed the maximum biomass yield and biomolecule accumulation compared to other organic carbon sources.

Suggested Citation

  • Rahul Kumar Goswami & Sanjeet Mehariya & Obulisamy Parthiba Karthikeyan & Pradeep Verma, 2022. "Influence of Carbon Sources on Biomass and Biomolecule Accumulation in Picochlorum sp. Cultured under the Mixotrophic Condition," IJERPH, MDPI, vol. 19(6), pages 1-18, March.
    • Handle: RePEc:gam:jijerp:v:19:y:2022:i:6:p:3674-:d:775130
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    References listed on IDEAS

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    1. Bhatnagar, Ashish & Chinnasamy, Senthil & Singh, Manjinder & Das, K.C., 2011. "Renewable biomass production by mixotrophic algae in the presence of various carbon sources and wastewaters," Applied Energy, Elsevier, vol. 88(10), pages 3425-3431.
    2. Dahmen-Ben Moussa, Ines & Chtourou, Haifa & Hassairi, Ilem & Sayadi, Sami & Dhouib, Abdelhafidh, 2019. "The effect of switching environmental conditions on content and structure of lipid produced by a wild strain Picochlorum sp," Renewable Energy, Elsevier, vol. 134(C), pages 406-415.
    3. Antonio Molino & Angela Iovine & Patrizia Casella & Sanjeet Mehariya & Simeone Chianese & Antonietta Cerbone & Juri Rimauro & Dino Musmarra, 2018. "Microalgae Characterization for Consolidated and New Application in Human Food, Animal Feed and Nutraceuticals," IJERPH, MDPI, vol. 15(11), pages 1-21, November.
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