IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v19y2022i15p9749-d882806.html
   My bibliography  Save this article

Wastewater Sequencing—An Innovative Method for Variant Monitoring of SARS-CoV-2 in Populations

Author

Listed:
  • Michal Tamáš

    (Department of Environmental Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radinského 9, 81237 Bratislava, Slovakia
    Institute of Physiology, Faculty of Medicine, Comenius University in Bratislava, 81372 Bratislava, Slovakia)

  • Alena Potocarova

    (Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University in Bratislava, Sasinkova 4, 81108 Bratislava, Slovakia)

  • Barbora Konecna

    (Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University in Bratislava, Sasinkova 4, 81108 Bratislava, Slovakia)

  • Ľubos Klucar

    (Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská Cesta 21, 84551 Bratislava, Slovakia)

  • Tomas Mackulak

    (Department of Environmental Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radinského 9, 81237 Bratislava, Slovakia)

Abstract

The SARS-CoV-2 outbreak has already affected more than 555 million people, and 6.3 million people have died. Due to its high infectivity, it is crucial to track SARS-CoV-2 outbreaks early to prevent the spread of infection. Wastewater monitoring appears to be a powerful and effective tool for managing epidemiological situations. Due to emerging mutations of SARS-CoV-2, there is a need to monitor mutations in order to control the pandemic. Since the sequencing of randomly chosen individuals is time-consuming and expensive, sequencing of wastewater plays an important role in revealing the dynamics of infection in a population. The sampling method used is a crucial factor and significantly impacts the results. Wastewater can be collected as a grab sample or as a 24 h composite sample. Another essential factor is the sample volume, as is the method of transport used. This review discusses different pretreatment procedures and RNA extraction, which may be performed using various methods, such as column-based extraction, TRIzol, or magnetic extraction. Each of the methods has its advantages and disadvantages, which are described accordingly. RT-qPCR is a procedure that confirms the presence of SARS-CoV-2 genes before sequencing. This review provides an overview of currently used methods for preparing wastewater samples, from sampling to sequencing.

Suggested Citation

  • Michal Tamáš & Alena Potocarova & Barbora Konecna & Ľubos Klucar & Tomas Mackulak, 2022. "Wastewater Sequencing—An Innovative Method for Variant Monitoring of SARS-CoV-2 in Populations," IJERPH, MDPI, vol. 19(15), pages 1-16, August.
    • Handle: RePEc:gam:jijerp:v:19:y:2022:i:15:p:9749-:d:882806
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/19/15/9749/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1660-4601/19/15/9749/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Hana Mlejnkova & Katerina Sovova & Petra Vasickova & Vera Ocenaskova & Lucie Jasikova & Eva Juranova, 2020. "Preliminary Study of Sars-Cov-2 Occurrence in Wastewater in the Czech Republic," IJERPH, MDPI, vol. 17(15), pages 1-9, July.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Tomáš Mackuľak & Miroslav Gál & Viera Špalková & Miroslav Fehér & Katarína Briestenská & Miriam Mikušová & Karolína Tomčíková & Michal Tamáš & Andrea Butor Škulcová, 2021. "Wastewater-Based Epidemiology as an Early Warning System for the Spreading of SARS-CoV-2 and Its Mutations in the Population," IJERPH, MDPI, vol. 18(11), pages 1-21, May.
    2. Parisa Aberi & Rezgar Arabzadeh & Heribert Insam & Rudolf Markt & Markus Mayr & Norbert Kreuzinger & Wolfgang Rauch, 2021. "Quest for Optimal Regression Models in SARS-CoV-2 Wastewater Based Epidemiology," IJERPH, MDPI, vol. 18(20), pages 1-17, October.
    3. Athmakuri Tharak & Harishankar Kopperi & Manupati Hemalatha & Uday Kiran & Gokulan C. G. & Shivranjani Moharir & Rakesh K. Mishra & S. Venkata Mohan, 2022. "Longitudinal and Long-Term Wastewater Surveillance for COVID-19: Infection Dynamics and Zoning of Urban Community," IJERPH, MDPI, vol. 19(5), pages 1-20, February.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:19:y:2022:i:15:p:9749-:d:882806. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.