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Preliminary Study of Sars-Cov-2 Occurrence in Wastewater in the Czech Republic

Author

Listed:
  • Hana Mlejnkova

    (T. G. Masaryk Water Research Institute, Public Research Institution, Podbabska 2582/30, 160 00 Prague, Czech Republic)

  • Katerina Sovova

    (T. G. Masaryk Water Research Institute, Public Research Institution, Brno Branch, Mojmirovo namesti 16, 612 00 Brno, Czech Republic)

  • Petra Vasickova

    (Veterinary Research Institute, Public Research Institution, Hudcova 296/70, 621 00 Brno, Czech Republic)

  • Vera Ocenaskova

    (T. G. Masaryk Water Research Institute, Public Research Institution, Podbabska 2582/30, 160 00 Prague, Czech Republic)

  • Lucie Jasikova

    (T. G. Masaryk Water Research Institute, Public Research Institution, Podbabska 2582/30, 160 00 Prague, Czech Republic)

  • Eva Juranova

    (T. G. Masaryk Water Research Institute, Public Research Institution, Podbabska 2582/30, 160 00 Prague, Czech Republic)

Abstract

The virus SARS-CoV-2, which has caused the recent COVID-19 pandemic, may be present in the stools of COVID-19 patients. Therefore, we aimed to detect SARS-CoV-2 in wastewater for surveillance of SARS-CoV-2 in the population. Samples of untreated wastewater were collected from 33 wastewater treatment plants (WWTPs) of different sizes within the Czech Republic. SARS-CoV-2 RNA was concentrated from wastewater and viral RNA was determined using real-time reverse transcription polymerase chain reaction (RT-qPCR). SARS-CoV-2 RNA was detected in 11.6% of samples and more than 27.3% of WWTPs; in some of them, SARS-CoV-2 was detected repeatedly. Our preliminary results indicate that an epidemiology approach that focuses on the determination of SARS-CoV-2 in wastewater could be suitable for SARS-CoV-2 surveillance in the population.

Suggested Citation

  • Hana Mlejnkova & Katerina Sovova & Petra Vasickova & Vera Ocenaskova & Lucie Jasikova & Eva Juranova, 2020. "Preliminary Study of Sars-Cov-2 Occurrence in Wastewater in the Czech Republic," IJERPH, MDPI, vol. 17(15), pages 1-9, July.
  • Handle: RePEc:gam:jijerp:v:17:y:2020:i:15:p:5508-:d:392380
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    Citations

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    Cited by:

    1. Parisa Aberi & Rezgar Arabzadeh & Heribert Insam & Rudolf Markt & Markus Mayr & Norbert Kreuzinger & Wolfgang Rauch, 2021. "Quest for Optimal Regression Models in SARS-CoV-2 Wastewater Based Epidemiology," IJERPH, MDPI, vol. 18(20), pages 1-17, October.
    2. Michal Tamáš & Alena Potocarova & Barbora Konecna & Ľubos Klucar & Tomas Mackulak, 2022. "Wastewater Sequencing—An Innovative Method for Variant Monitoring of SARS-CoV-2 in Populations," IJERPH, MDPI, vol. 19(15), pages 1-16, August.
    3. Tomáš Mackuľak & Miroslav Gál & Viera Špalková & Miroslav Fehér & Katarína Briestenská & Miriam Mikušová & Karolína Tomčíková & Michal Tamáš & Andrea Butor Škulcová, 2021. "Wastewater-Based Epidemiology as an Early Warning System for the Spreading of SARS-CoV-2 and Its Mutations in the Population," IJERPH, MDPI, vol. 18(11), pages 1-21, May.
    4. Athmakuri Tharak & Harishankar Kopperi & Manupati Hemalatha & Uday Kiran & Gokulan C. G. & Shivranjani Moharir & Rakesh K. Mishra & S. Venkata Mohan, 2022. "Longitudinal and Long-Term Wastewater Surveillance for COVID-19: Infection Dynamics and Zoning of Urban Community," IJERPH, MDPI, vol. 19(5), pages 1-20, February.

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