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Mouthwash Based on Ozonated Olive Oil in Caries Prevention: A Preliminary In-Vitro Study

Author

Listed:
  • Gianna Maria Nardi

    (Department of Dental and Maxillofacial Sciences, Sapienza University of Rome, 00161 Rome, Italy)

  • Sara Fais

    (Department of Surgical Sciences, Oral Biotechnology Laboratory, University of Cagliari, 09124 Cagliari, Italy)

  • Cinzia Casu

    (Department of Surgical Sciences, Oral Biotechnology Laboratory, University of Cagliari, 09124 Cagliari, Italy)

  • Marta Mazur

    (Department of Dental and Maxillofacial Sciences, Sapienza University of Rome, 00161 Rome, Italy)

  • Roberto Di Giorgio

    (Department of Dental and Maxillofacial Sciences, Sapienza University of Rome, 00161 Rome, Italy)

  • Roberta Grassi

    (Department of Biomedical Sciences, University of Sassari, 07100 Sassari, Italy)

  • Felice Roberto Grassi

    (Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari Aldo Moro, 70122 Bari, Italy)

  • Germano Orrù

    (Department of Surgical Sciences, Oral Biotechnology Laboratory, University of Cagliari, 09124 Cagliari, Italy
    National Research Council of Italy, ISPA-CNR, 07100 Sassari, Italy)

Abstract

(1) Background: Ozone (O3) proved to oxidize organic and inorganic compounds, and its efficacy against bacteria, viruses and fungi plasma membranes was of interest. Ozone vehicle can be a gaseous form, ozonated water or ozonized oil. The aim of this in-vitro study was to evaluate the efficacy of ozonated olive oil against Streptococcus mutans . (2) Methods: Two different commercial mouthwashes were tested: Ialozon Blu (IB) (Gemavip, Cagliari, Italy), with ozonated olive oil, and Ialozon Rose (IR) (Gemavip, Cagliari, Italy), with ozonated olive oil, hyaluronic acid and vitamin E. All formulates were analyzed in a dilution range from 2- to 256-folds in saline solution, as to reproduce the salivary dilution. Streptococcus mutans CIP103220 strain was used for the antimicrobial susceptibility test, and the Kirby–Bauer inhibition method was performed to evaluate the Minimum Inhibitory (MIC), Minimum Bactericidal (MBC), and Minimum Biofilm Inhibitory Concentration (MBIC). (3) Results: Both formulates showed the same antimicrobial activity. MIC, MBC, and MBIC were observed for dilution factors of 1/32, 1/8 and 1/8, respectively. The mean value of inhibition zone diameter was 16.5 mm for IB, and 18 mm for IR. (4) Conclusions: The results suggested that ozonized olive oil formulates were able to inactivate Streptococcus mutans avoiding the salivary dilution effect in the oral cavity.

Suggested Citation

  • Gianna Maria Nardi & Sara Fais & Cinzia Casu & Marta Mazur & Roberto Di Giorgio & Roberta Grassi & Felice Roberto Grassi & Germano Orrù, 2020. "Mouthwash Based on Ozonated Olive Oil in Caries Prevention: A Preliminary In-Vitro Study," IJERPH, MDPI, vol. 17(23), pages 1-9, December.
    • Handle: RePEc:gam:jijerp:v:17:y:2020:i:23:p:9106-:d:457617
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    References listed on IDEAS

    as
    1. Gianna Maria Nardi & Francesca Cesarano & Giulio Papa & Lorella Chiavistelli & Roman Ardan & Maciej Jedlinski & Marta Mazur & Roberta Grassi & Felice Roberto Grassi, 2020. "Evaluation of Salivary Matrix Metalloproteinase (MMP-8) in Periodontal Patients Undergoing Non-Surgical Periodontal Therapy and Mouthwash Based on Ozonated Olive Oil: A Randomized Clinical Trial," IJERPH, MDPI, vol. 17(18), pages 1-10, September.
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    Cited by:

    1. Luís Azevedo & André Correia & Carlos F. Almeida & Pedro Molinero-Mourelle & Maria Correia & Jaime Del Río Highsmith, 2021. "Biocompatibility and Effectiveness of a Novel, Organic Olive Oil-Based Denture Adhesive: A Multicenter Randomized and Placebo-Controlled Clinical Trial," IJERPH, MDPI, vol. 18(7), pages 1-11, March.

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