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Combined Effect of Diosgenin Along with Ezetimibe or Atorvastatin on the Fate of Labelled Bile Acid and Cholesterol in Hypercholesterolemic Rats

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  • Alejandro Marín-Medina

    (División Académica de Ciencias de la Salud, Universidad Juárez Autónoma de Tabasco (UJAT), Villahermosa C.P. 86100, Tabasco, Mexico
    Universidad de Guadalajara, CUCS, Guadalajara C.P. 44340, Jalisco, Mexico)

  • Gonzalo Ruíz-Hidalgo

    (Hospital Angeles de Villahermosa, Villahermosa C.P. 86035, Tabasco, Mexico)

  • Jorge L. Blé-Castillo

    (División Académica de Ciencias de la Salud, Universidad Juárez Autónoma de Tabasco (UJAT), Villahermosa C.P. 86100, Tabasco, Mexico)

  • Alma M. Zetina-Esquivel

    (División Académica de Ciencias de la Salud, Universidad Juárez Autónoma de Tabasco (UJAT), Villahermosa C.P. 86100, Tabasco, Mexico)

  • Rodrigo Miranda Zamora

    (Facultad de Medicina, División de Investigación, Departamento de Bioquímica, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México C.P. 04510, Ciudad de México, Mexico)

  • Isela E. Juárez-Rojop

    (División Académica de Ciencias de la Salud, Universidad Juárez Autónoma de Tabasco (UJAT), Villahermosa C.P. 86100, Tabasco, Mexico)

  • Juan C. Díaz-Zagoya

    (Facultad de Medicina, División de Investigación, Departamento de Bioquímica, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México C.P. 04510, Ciudad de México, Mexico)

Abstract

We analyzed the effect of diosgenin, administered with atorvastatin or ezetimibe, on the fate of 3 H(G)-taurocholic acid or 26- 14 C-cholesterol in hypercholesterolemic rats. Male Wistar rats received a hypercholesterolemic diet (HD), HD + atorvastatin (HD+ATV), HD + ezetimibe (HD+EZT), HD + diosgenin (HD+DG), HD+ATV+EZT, or HD+ATV+DG for 40 days. We also included a control normal group (ND). The labelled compounds were administered on day 30. The animals were placed in metabolic cages for daily feces collection. At day 40 the rats were sacrificed. Lipid extracts from blood, liver, spinal cord, testicles, kidneys, epididymis, intestine, and feces were analyzed for radioactivity. Cholesterol activity was the highest in the liver in HD rats. DG diminished one half of this activity in HD+DG and HD+ATV+DG groups in comparison with the HD group. HD+ATV rats showed four to almost ten-fold cholesterol activity in the spinal cord compared with the ND or HD rats. Fecal elimination of neutral steroids was approximately two-fold higher in the HD+DG and HD+ATV+DG groups. Taurocholic acid activity was four to ten-fold higher in HD+DG intestine as compared to the other experimental groups. Taurocholic activity in the liver of HD and HD+DG groups was two and a half higher than in ND. Our results show that the combination of DG and ATV induced the highest cholesterol reduction in the liver and other tissues.

Suggested Citation

  • Alejandro Marín-Medina & Gonzalo Ruíz-Hidalgo & Jorge L. Blé-Castillo & Alma M. Zetina-Esquivel & Rodrigo Miranda Zamora & Isela E. Juárez-Rojop & Juan C. Díaz-Zagoya, 2019. "Combined Effect of Diosgenin Along with Ezetimibe or Atorvastatin on the Fate of Labelled Bile Acid and Cholesterol in Hypercholesterolemic Rats," IJERPH, MDPI, vol. 16(4), pages 1-10, February.
    • Handle: RePEc:gam:jijerp:v:16:y:2019:i:4:p:627-:d:207775
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    References listed on IDEAS

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    1. Jyh-Yeuan Lee & Lisa N. Kinch & Dominika M. Borek & Jin Wang & Junmei Wang & Ina L. Urbatsch & Xiao-Song Xie & Nikolai V. Grishin & Jonathan C. Cohen & Zbyszek Otwinowski & Helen H. Hobbs & Daniel M. , 2016. "Crystal structure of the human sterol transporter ABCG5/ABCG8," Nature, Nature, vol. 533(7604), pages 561-564, May.
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