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Characterization of Bacterial Communities and Their Antibiotic Resistance Profiles in Wastewaters Obtained from Pharmaceutical Facilities in Lagos and Ogun States, Nigeria

Author

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  • Amarachukwu Obayiuwana

    (Department of Biological Sciences, Augustine University Ilara, Epe 106101, Lagos State, Nigeria)

  • Adeniyi Ogunjobi

    (Department of Microbiology, University of Ibadan, Ibadan 200284, Oyo State, Nigeria)

  • Min Yang

    (State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China)

  • Mark Ibekwe

    (U.S. Salinity Laboratory, USDA-ARS, 450 West Big Springs Road, Riverside, CA 92507, USA)

Abstract

In Nigeria, pharmaceutical wastewaters are routinely disseminated in river waters; this could be associated with public health risk to humans and animals. In this study, we characterized antibiotic resistant bacteria (ARB) and their antibiotic resistance profile as well as screening for sul 1 and sul 2 genes in pharmaceutical wastewater effluents. Bacterial composition of the wastewater sources was isolated on non-selective media and characterized by the polymerase chain reaction (PCR) amplification of the 16S rRNA genes, with subsequent grouping using restriction fragment length polymorphism (RFLP) and sequencing. The antibiotics sensitivity profiles were investigated using the standard disk diffusion plate method and the minimum inhibitory concentrations (MICs) of selected antibiotics on the bacterial isolates. A total of 254 bacterial strains were isolated, and majority of the isolates were identified as Acinetobacter sp., Klebsiella pneumonia , Proteus mirabilis , Enterobacter sp. and Bacillus sp. A total of 218 (85.8%) of the bacterial isolates were multidrug resistant. High MICs values were observed for all antibiotics used in the study. The result showed that 31.7%, 21.7% and 43.3% of the bacterial isolates harbored sul 1, sul 2, and Intl 1 genes, respectively. Pharmaceuticals wastewaters are potential reservoirs of ARBs which may harbor resistance genes with possible risk to public health.

Suggested Citation

  • Amarachukwu Obayiuwana & Adeniyi Ogunjobi & Min Yang & Mark Ibekwe, 2018. "Characterization of Bacterial Communities and Their Antibiotic Resistance Profiles in Wastewaters Obtained from Pharmaceutical Facilities in Lagos and Ogun States, Nigeria," IJERPH, MDPI, vol. 15(7), pages 1-14, June.
    • Handle: RePEc:gam:jijerp:v:15:y:2018:i:7:p:1365-:d:155187
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    References listed on IDEAS

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    1. La Thi Quynh Lien & Pham Thi Lan & Nguyen Thi Kim Chuc & Nguyen Quynh Hoa & Pham Hong Nhung & Nguyen Thi Minh Thoa & Vishal Diwan & Ashok J. Tamhankar & Cecilia Stålsby Lundborg, 2017. "Antibiotic Resistance and Antibiotic Resistance Genes in Escherichia coli Isolates from Hospital Wastewater in Vietnam," IJERPH, MDPI, vol. 14(7), pages 1-11, June.
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    Cited by:

    1. Alexandra Bastaraud & Philippe Cecchi & Pascal Handschumacher & Mathias Altmann & Ronan Jambou, 2020. "Urbanization and Waterborne Pathogen Emergence in Low-Income Countries: Where and How to Conduct Surveys?," IJERPH, MDPI, vol. 17(2), pages 1-19, January.
    2. Xuexia Yuan & Yong Zhang & Chenxi Sun & Wenbo Wang & Yuanjuan Wu & Lixia Fan & Bing Liu, 2022. "Profile of Bacterial Community and Antibiotic Resistance Genes in Typical Vegetable Greenhouse Soil," IJERPH, MDPI, vol. 19(13), pages 1-15, June.

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