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Frontal Cortex Transcriptome Analysis of Mice Exposed to Electronic Cigarettes During Early Life Stages

Author

Listed:
  • Dana E. Lauterstein

    (Department of Environmental Medicine, New York University School of Medicine, Tuxedo, NY 10987, USA)

  • Pamella B. Tijerina

    (Department of Environmental Medicine, New York University School of Medicine, Tuxedo, NY 10987, USA)

  • Kevin Corbett

    (Department of Environmental Medicine, New York University School of Medicine, Tuxedo, NY 10987, USA)

  • Betul Akgol Oksuz

    (Genome Technology Center, New York University School of Medicine, New York, NY 10016, USA)

  • Steven S. Shen

    (Department of Environmental Medicine, New York University School of Medicine, Tuxedo, NY 10987, USA
    Genome Technology Center, New York University School of Medicine, New York, NY 10016, USA
    Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY 10016, USA)

  • Terry Gordon

    (Department of Environmental Medicine, New York University School of Medicine, Tuxedo, NY 10987, USA)

  • Catherine B. Klein

    (Department of Environmental Medicine, New York University School of Medicine, Tuxedo, NY 10987, USA)

  • Judith T. Zelikoff

    (Department of Environmental Medicine, New York University School of Medicine, Tuxedo, NY 10987, USA)

Abstract

Electronic cigarettes (e-cigarettes), battery-powered devices containing nicotine, glycerin, propylene glycol, flavorings, and other substances, are increasing in popularity. They pose a potential threat to the developing brain, as nicotine is a known neurotoxicant. We hypothesized that exposure to e-cigarettes during early life stages induce changes in central nervous system (CNS) transcriptome associated with adverse neurobiological outcomes and long-term disease states. To test the hypothesis, pregnant C57BL/6 mice were exposed daily (via whole body inhalation) throughout gestation (3 h/day; 5 days/week) to aerosols produced from e-cigarettes either with nicotine (13–16 mg/mL) or without nicotine; following birth, pups and dams were exposed together to e-cigarette aerosols throughout lactation beginning at postnatal day (PND) 4–6 and using the same exposure conditions employed during gestational exposure. Following exposure, frontal cortex recovered from ~one-month-old male and female offspring were excised and analyzed for gene expression by RNA Sequencing (RNA-Seq). Comparisons between the treatment groups revealed that e-cigarette constituents other than nicotine might be partly responsible for the observed biological effects. Transcriptome alterations in both offspring sexes and treatment groups were all significantly associated with downstream adverse neurobiological outcomes. Results from this study demonstrate that e-cigarette exposure during early life alters CNS development potentially leading to chronic neuropathology.

Suggested Citation

  • Dana E. Lauterstein & Pamella B. Tijerina & Kevin Corbett & Betul Akgol Oksuz & Steven S. Shen & Terry Gordon & Catherine B. Klein & Judith T. Zelikoff, 2016. "Frontal Cortex Transcriptome Analysis of Mice Exposed to Electronic Cigarettes During Early Life Stages," IJERPH, MDPI, vol. 13(4), pages 1-14, April.
  • Handle: RePEc:gam:jijerp:v:13:y:2016:i:4:p:417-:d:68030
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    References listed on IDEAS

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    1. Manabu Fuchikami & Shigeru Morinobu & Masahiro Segawa & Yasumasa Okamoto & Shigeto Yamawaki & Norio Ozaki & Takeshi Inoue & Ichiro Kusumi & Tsukasa Koyama & Kounosuke Tsuchiyama & Takeshi Terao, 2011. "DNA Methylation Profiles of the Brain-Derived Neurotrophic Factor (BDNF) Gene as a Potent Diagnostic Biomarker in Major Depression," PLOS ONE, Public Library of Science, vol. 6(8), pages 1-7, August.
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    Citations

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    Cited by:

    1. Kayla Rae Farrell & Emma Karey & Shu Xu & Grace Gibbon & Terry Gordon & Michael Weitzman, 2021. "E-Cigarette Use, Systemic Inflammation, and Depression," IJERPH, MDPI, vol. 18(19), pages 1-13, October.
    2. Sung-Hyun Park & Yuting Lu & Yongzhao Shao & Colette Prophete & Lori Horton & Maureen Sisco & Hyun-Wook Lee & Thomas Kluz & Hong Sun & Max Costa & Judith Zelikoff & Lung-Chi Chen & Matthew W. Gorr & L, 2022. "Longitudinal Impact of WTC Dust Inhalation on Rat Cardiac Tissue Transcriptomic Profiles," IJERPH, MDPI, vol. 19(2), pages 1-16, January.
    3. Sebastian Sailer & Giorgia Sebastiani & Vicente Andreu-Férnández & Oscar García-Algar, 2019. "Impact of Nicotine Replacement and Electronic Nicotine Delivery Systems on Fetal Brain Development," IJERPH, MDPI, vol. 16(24), pages 1-17, December.
    4. Alexandra Noël & Sultan Yilmaz & Tori Farrow & Matthew Schexnayder & Oliver Eickelberg & Tomislav Jelesijevic, 2023. "Sex-Specific Alterations of the Lung Transcriptome at Birth in Mouse Offspring Prenatally Exposed to Vanilla-Flavored E-Cigarette Aerosols and Enhanced Susceptibility to Asthma," IJERPH, MDPI, vol. 20(4), pages 1-20, February.

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