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Prevalence of Genes of OXA-23 Carbapenemase and AdeABC Efflux Pump Associated with Multidrug Resistance of Acinetobacter baumannii Isolates in the ICU of a Comprehensive Hospital of Northwestern China

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  • Wei Jia

    (Ningxia Key laboratory of Clinical and Pathogenic Microbiology, Yinchuan, Ningxia 750004, China
    Center of Laboratory Medicine, the General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, China)

  • Caiyun Li

    (Ningxia Key laboratory of Clinical and Pathogenic Microbiology, Yinchuan, Ningxia 750004, China
    The Graduate school, Ningxia Medical University, Yinchuan, Ningxia 750004, China)

  • Haiyun Zhang

    (Center of Laboratory Medicine, the General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, China
    The First People's Hospital of Mudanjiang City, Mudanjiang, Heilongjiang 157011, China)

  • Gang Li

    (Ningxia Key laboratory of Clinical and Pathogenic Microbiology, Yinchuan, Ningxia 750004, China
    Center of Laboratory Medicine, the General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, China)

  • Xiaoming Liu

    (Ningxia Key laboratory of Clinical and Pathogenic Microbiology, Yinchuan, Ningxia 750004, China)

  • Jun Wei

    (Ningxia Key laboratory of Clinical and Pathogenic Microbiology, Yinchuan, Ningxia 750004, China
    Center of Laboratory Medicine, the General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, China)

Abstract

The objective of this study was to explore the molecular epidemiology and the genetic support of clinical multidrug resistant (MDR) Acinetobacter baumannii (A. baumannii ) isolates in an ICU ward of a comprehensive hospital. A total of 102 non-duplicate drug-resistant A. baumannii isolates were identified and 93 (91.1%) of them were MDR strains. Molecular analysis demonstrated that carbapenemase genes bla OXA -23 and bla OXA -51 were presented in all 93 MDR isolates (100%), but other carbapenemase genes, including bla OXA -24 , bla OXA -58 , bla IMP-1 , bla IMP-4 , bla SIM , and bla VIM genes were completely absent in all isolates. In addition, genes of AdeABC efflux system were detected in 88.2% (90/102) isolates. Interestingly, an addition to efflux pump inhibitor, reserpine could significantly enhance the susceptibility of MDR isolates to moxifloxacin, cefotaxime, and imipenem ( p < 0.01). Clonal relationship analysis further grouped these clinical drug-resistant isolates into nine clusters, and the MDR strains were mainly in clusters A, B, C, and D, which include 16, 13, 25, and 15 isolates, respectively. This study demonstrated that clinical isolates carrying carbapenemase-encoding genes bla OXA -23 and AdeABC efflux pump genes are the main prevalent MDR A. baumannii , and the co-expression of oxacillinase and efflux pump proteins are thus considered to be the important reason for the prevalence of this organism in the ICU of this hospital.

Suggested Citation

  • Wei Jia & Caiyun Li & Haiyun Zhang & Gang Li & Xiaoming Liu & Jun Wei, 2015. "Prevalence of Genes of OXA-23 Carbapenemase and AdeABC Efflux Pump Associated with Multidrug Resistance of Acinetobacter baumannii Isolates in the ICU of a Comprehensive Hospital of Northwestern China," IJERPH, MDPI, vol. 12(8), pages 1-14, August.
    • Handle: RePEc:gam:jijerp:v:12:y:2015:i:8:p:10079-10092:d:54591
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    References listed on IDEAS

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    1. Wei Jia & Gang Li & Wen Wang, 2014. "Prevalence and Antimicrobial Resistance of Enterococcus Species: A Hospital-Based Study in China," IJERPH, MDPI, vol. 11(3), pages 1-19, March.
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