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Characterization of Aminoglycoside Resistance and Virulence Genes among Enterococcus spp. Isolated from a Hospital in China

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  • Wanxiang Li

    (Center of Laboratory Medicine, Zhejiang Provincial People's Hospital, 158 Shangtang Road, Hangzhou 310014, China
    Second Clinical Medical College, Zhejiang Chinese Medical University, 548 Binwen Road, Hangzhou 310053, China
    These authors contributed equally to this work.)

  • Jing Li

    (Medical College, Shihezi University, 280 Beisan Road, Shihezi 832000, China
    These authors contributed equally to this work.)

  • Quhao Wei

    (Center of Laboratory Medicine, Zhejiang Provincial People's Hospital, 158 Shangtang Road, Hangzhou 310014, China)

  • Qingfeng Hu

    (Center of Laboratory Medicine, Zhejiang Provincial People's Hospital, 158 Shangtang Road, Hangzhou 310014, China)

  • Xiaowei Lin

    (Second Clinical Medical College, Zhejiang Chinese Medical University, 548 Binwen Road, Hangzhou 310053, China)

  • Mengquan Chen

    (Wenzhou Hospital of Traditional Chinese Medicine, 27 Dashimen Road Xinhe Street, Wenzhou 325000, China)

  • Renji Ye

    (Medical College, Taizhou University, 1139 Shifu Road, Taizhou 318000, China)

  • Huoyang Lv

    (Center of Laboratory Medicine, Zhejiang Provincial People's Hospital, 158 Shangtang Road, Hangzhou 310014, China)

Abstract

This study investigated the aminoglycoside resistance phenotypes and genotypes, as well as the prevalence of virulence genes, in Enterococcus species isolated from clinical patients in China. A total of 160 enterococcal isolates from various clinical samples collected from September 2013 to July 2014 were identified to the species level using the VITEK-2 COMPACT system. The antimicrobial susceptibilities of the identified Enterococcus strains were determined by the Kirby-Bauer (K-B) disc diffusion method. PCR-based assays were used to detect the aminoglycoside resistance and virulence genes in all enterococcal isolates. Of 160 Enterococcus isolates, 105 were identified as E. faecium , 35 as E. faecalis , and 20 isolates were classified as “other” Enterococcus species. High-level aminoglycoside resistance (HLAR) for gentamicin, streptomycin, and both antibiotics was identified in 58.8, 50, and 34.4% of strains, respectively. The most common virulence gene (50.6% of isolates) was efaA , followed by asa1 (28.8%). The most prevalent aminoglycoside resistance genes were aac(6')-Ie-aph(2'') , aph(2')-Id , aph(3')-IIIa , and ant(6')-Ia , present in 49.4%, 1.3%, 48.8% and 31.3% of strains, respectively. Overall, E. faecium and E. faecalis were most frequently associated with hospital-acquired enterococcal infections in Zhejiang Province. All aminoglycoside resistance genes, except aph ( 2'' ) -Id , were significantly more prevalent in HLAR strains than amongst high level aminoglycoside susceptible (HLAS) strains, while there was no significant difference between HLAR and HLAS strains in regard to the prevalence of virulence genes, apart from esp , therefore, measures should be taken to manage infections caused by multi-drug resistant Enterococcus species.

Suggested Citation

  • Wanxiang Li & Jing Li & Quhao Wei & Qingfeng Hu & Xiaowei Lin & Mengquan Chen & Renji Ye & Huoyang Lv, 2015. "Characterization of Aminoglycoside Resistance and Virulence Genes among Enterococcus spp. Isolated from a Hospital in China," IJERPH, MDPI, vol. 12(3), pages 1-12, March.
    • Handle: RePEc:gam:jijerp:v:12:y:2015:i:3:p:3014-3025:d:46654
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    References listed on IDEAS

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    1. Wei Jia & Gang Li & Wen Wang, 2014. "Prevalence and Antimicrobial Resistance of Enterococcus Species: A Hospital-Based Study in China," IJERPH, MDPI, vol. 11(3), pages 1-19, March.
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