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Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation

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  • Yoon Hee Cho

    (Department of Molecular Epidemiology, School of Public Health, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Korea
    Current address: Department of Biomedical and Pharmaceutical Sciences, College of Health Professions and Biomedical Sciences, University of Montana, 32 Campus Drive, Missoula, MT 59812, USA
    These authors contributed equally to this work.)

  • Su Young Kim

    (Department of Preventive Medicine, School of Medicine, Jeju National University, 66 Jejudaehakno, Jeju-si, Jeju-do 690-756, Korea
    These authors contributed equally to this work.)

  • Hae Dong Woo

    (Molecular Epidemiology Branch, Division of Cancer Epidemiology and Prevention, Research Institute, National Cancer Center, Goyang-si, Gyeonggi-do 410-769, Korea)

  • Yang Jee Kim

    (Da Vinci College of General Education, Chung-ang University, 84 HeukSeok-Ro, DongJak-gu, Seoul 156-756, Korea)

  • Sung Whan Ha

    (Department of Radiation Oncology, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul 110-744, Korea)

  • Hai Won Chung

    (Department of Molecular Epidemiology, School of Public Health, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Korea)

Abstract

Radiation-induced genomic instability refers to a type of damage transmitted over many generations following irradiation. This delayed impact of radiation exposure may pose a high risk to human health and increases concern over the dose limit of radiation exposure for both the public and radiation workers. Therefore, the development of additional biomarkers is still needed for the detection of delayed responses following low doses of radiation exposure. In this study, we examined the effect of X-irradiation on delayed induction of numerical chromosomal aberrations in normal human fibroblasts irradiated with 20, 50 and 100 cGy of X-rays using the micronucleus-centromere assay. Frequencies of centromere negative- and positive-micronuclei, and aneuploidy of chromosome 1 and 4 were analyzed in the surviving cells at 28, 88 and 240 h after X-irradiation. X-irradiation increased the frequency of micronuclei (MN) in a dose-dependent manner in the cells at all measured time-points, but no significant differences in MN frequency among cell passages were observed. Aneuploid frequency of chromosomes 1 and 4 increased with radiation doses, and a significantly higher frequency of aneuploidy was observed in the surviving cells analyzed at 240 h compared to 28 h. These results indicate that low-dose of X-irradiation can induce delayed aneuploidy of chromosomes 1 and 4 in normal fibroblasts.

Suggested Citation

  • Yoon Hee Cho & Su Young Kim & Hae Dong Woo & Yang Jee Kim & Sung Whan Ha & Hai Won Chung, 2015. "Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation," IJERPH, MDPI, vol. 12(12), pages 1-11, December.
    • Handle: RePEc:gam:jijerp:v:12:y:2015:i:12:p:14979-15172:d:59675
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    References listed on IDEAS

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    1. Christoph Lengauer & Kenneth W. Kinzler & Bert Vogelstein, 1998. "Genetic instabilities in human cancers," Nature, Nature, vol. 396(6712), pages 643-649, December.
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