IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v11y2014i12p13130-13143d43665.html
   My bibliography  Save this article

Protective Roles of Sodium Selenite against Aflatoxin B 1 -Induced Apoptosis of Jejunum in Broilers

Author

Listed:
  • Xi Peng

    (Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China
    These authors contributed equally to this study.)

  • Shengqiang Zhang

    (Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China
    These authors contributed equally to this study.)

  • Jing Fang

    (Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China)

  • Hengmin Cui

    (Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China)

  • Zhicai Zuo

    (Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China)

  • Junliang Deng

    (Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China)

Abstract

The effects of aflatoxin B 1 (AFB 1 ) exposure and sodium selenite supplementation on cell apoptosis of jejunum in broilers were studied. A total of 240 one-day-old male AA broilers were randomly assigned four dietary treatments containing 0 mg/kg of AFB 1 (control), 0.3 mg/kg AFB 1 (AFB 1 ), 0.4 mg/kg supplement Se (+ Se) and 0.3 mg/kg AFB 1 + 0.4 mg/kg supplement Se (AFB 1 + Se), respectively. Compared with the control broilers, the number of apoptotic cells, the expression of Bax and Caspase-3 mRNA were significantly increased, while the expression of Bcl-2 mRNA and the Bcl-2/Bax ratio were significantly decreased in AFB 1 broilers. The number of apoptotic cells and the expression of Caspase-3 mRNA in AFB 1 + Se broilers were significantly higher than those in the control broilers, but significantly lower than those in AFB 1 broilers. There were no significant changes in the expression of Bax mRNA between AFB 1 + Se and control broilers; the expression of Bcl-2 mRNA and the Bcl-2/Bax ratio in AFB 1 + Se broilers were significantly lower than those in the control broilers, but significantly higher than those in AFB 1 broilers. In conclusion, 0.3 mg/kg AFB 1 in the diet can increase cell apoptosis, decrease Bcl-2 mRNA expression, and increase of Bax and Caspase-3 mRNA expression in broiler’s jejunum. However, supplementation of dietary sodium selenite at the concentration of 0.4 mg/kg Se may ameliorate AFB 1 -induced apoptosis by increasing Bcl-2 mRNA expression, and decreasing Bax and Caspase-3 mRNA expression.

Suggested Citation

  • Xi Peng & Shengqiang Zhang & Jing Fang & Hengmin Cui & Zhicai Zuo & Junliang Deng, 2014. "Protective Roles of Sodium Selenite against Aflatoxin B 1 -Induced Apoptosis of Jejunum in Broilers," IJERPH, MDPI, vol. 11(12), pages 1-14, December.
  • Handle: RePEc:gam:jijerp:v:11:y:2014:i:12:p:13130-13143:d:43665
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/11/12/13130/pdf
    Download Restriction: no

    File URL: https://www.mdpi.com/1660-4601/11/12/13130/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Fengyuan Wang & Gang Shu & Xi Peng & Jing Fang & Kejie Chen & Hengmin Cui & Zhengli Chen & Zhicai Zuo & Junliang Deng & Yi Geng & Weimin Lai, 2013. "Protective Effects of Sodium Selenite against Aflatoxin B 1 -Induced Oxidative Stress and Apoptosis in Broiler Spleen," IJERPH, MDPI, vol. 10(7), pages 1-11, July.
    2. Jianying Huang & Hengmin Cui & Xi Peng & Jing Fang & Zhicai Zuo & Junliang Deng & Bangyuan Wu, 2013. "The Association between Splenocyte Apoptosis and Alterations of Bax, Bcl-2 and Caspase-3 mRNA Expression, and Oxidative Stress Induced by Dietary Nickel Chloride in Broilers," IJERPH, MDPI, vol. 10(12), pages 1-17, December.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Na Liang & Fengyuan Wang & Xi Peng & Jing Fang & Hengmin Cui & Zhengli Chen & Weimin Lai & Yi Zhou & Yi Geng, 2015. "Effect of Sodium Selenite on Pathological Changes and Renal Functions in Broilers Fed a Diet Containing Aflatoxin B 1," IJERPH, MDPI, vol. 12(9), pages 1-13, September.
    2. Xi Peng & Shiping Bai & Xuemei Ding & Keying Zhang, 2017. "Pathological Impairment, Cell Cycle Arrest and Apoptosis of Thymus and Bursa of Fabricius Induced by Aflatoxin-Contaminated Corn in Broilers," IJERPH, MDPI, vol. 14(1), pages 1-11, January.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Na Liang & Fengyuan Wang & Xi Peng & Jing Fang & Hengmin Cui & Zhengli Chen & Weimin Lai & Yi Zhou & Yi Geng, 2015. "Effect of Sodium Selenite on Pathological Changes and Renal Functions in Broilers Fed a Diet Containing Aflatoxin B 1," IJERPH, MDPI, vol. 12(9), pages 1-13, September.
    2. Schneider, Kate R., 2022. "Nationally representative estimates of the cost of adequate diets, nutrient level drivers, and policy options for households in rural Malawi," Food Policy, Elsevier, vol. 113(C).
    3. Ali Allam & Ahmed Abdeen & Hari Prasad Devkota & Samar S. Ibrahim & Gehan Youssef & Ahmed Soliman & Mohamed M. Abdel-Daim & Khalid J. Alzahrani & Khaled Shoghy & Samah F. Ibrahim & Mohamed Aboubakr, 2022. "N -Acetylcysteine Alleviated the Deltamethrin-Induced Oxidative Cascade and Apoptosis in Liver and Kidney Tissues," IJERPH, MDPI, vol. 19(2), pages 1-13, January.
    4. Noreddine Benkerroum, 2019. "Retrospective and Prospective Look at Aflatoxin Research and Development from a Practical Standpoint," IJERPH, MDPI, vol. 16(19), pages 1-47, September.
    5. Kejie Chen & Xi Peng & Jing Fang & Hengmin Cui & Zhicai Zuo & Junliang Deng & Zhengli Chen & Yi Geng & Weimin Lai & Li Tang & Qingqiu Yang, 2014. "Effects of Dietary Selenium on Histopathological Changes and T Cells of Spleen in Broilers Exposed to Aflatoxin B 1," IJERPH, MDPI, vol. 11(2), pages 1-10, February.
    6. Noreddine Benkerroum, 2020. "Chronic and Acute Toxicities of Aflatoxins: Mechanisms of Action," IJERPH, MDPI, vol. 17(2), pages 1-28, January.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:11:y:2014:i:12:p:13130-13143:d:43665. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.