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Occurrence of Virulence Genes Associated with Diarrheagenic Escherichia coli Isolated from Raw Cow’s Milk from Two Commercial Dairy Farms in the Eastern Cape Province, South Africa

Author

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  • Lesley-Anne Caine

    (Departments of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa)

  • Uchechukwu U. Nwodo

    (Departments of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa)

  • Anthony I. Okoh

    (Departments of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa)

  • Roland N. Ndip

    (Departments of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa
    Department of Microbiology and Parasitology, Faculty of Science, University of Buea, P.O. Box 63, Buea, Cameroon)

  • Ezekiel Green

    (Departments of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa)

Abstract

Escherichia coli remains a public health concern worldwide as an organism that causes diarrhea and its reservoir in raw milk may play an important role in the survival and transport of pathogenic strains. Diarrheagenic E. coli strains are diverse food-borne pathogens and causes diarrhea with varying virulence in humans. We investigated the prevalence of pathogenic E. coli in raw milk from two commercial dairy farms. Four hundred raw milk samples, 200 from each dairy farm, were screened for the presence of fliCH7, eagR, ial, eagg, lt, and papC genes . In dairy farm A, 100 E. coli were identified based on culture, oxidase and Gram staining, while 88 isolates from dairy farm B were identified in the same manner. Gene detection showed fliCH7 27 (54%) to be the highest gene detected from farm A and lt 2 (4%) to be the lowest. The highest gene detected in dairy farm B was fliCH7 16 (43.2%) and papC 1 (2.7%) was the least. The amplification of pathogenic genes associated with diarrheagenic E. coli from cows’ raw milk demonstrates that potentially virulent E. coli strains are widely distributed in raw milk and may be a cause of concern for human health.

Suggested Citation

  • Lesley-Anne Caine & Uchechukwu U. Nwodo & Anthony I. Okoh & Roland N. Ndip & Ezekiel Green, 2014. "Occurrence of Virulence Genes Associated with Diarrheagenic Escherichia coli Isolated from Raw Cow’s Milk from Two Commercial Dairy Farms in the Eastern Cape Province, South Africa," IJERPH, MDPI, vol. 11(11), pages 1-14, November.
    • Handle: RePEc:gam:jijerp:v:11:y:2014:i:11:p:11950-11963:d:42444
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    Citations

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    Cited by:

    1. Akebe Luther King Abia & Lisa Schaefer & Eunice Ubomba-Jaswa & Wouter Le Roux, 2017. "Abundance of Pathogenic Escherichia coli Virulence-Associated Genes in Well and Borehole Water Used for Domestic Purposes in a Peri-Urban Community of South Africa," IJERPH, MDPI, vol. 14(3), pages 1-11, March.
    2. Abongile Pekana & Ezekiel Green, 2018. "Antimicrobial Resistance Profiles of Staphylococcus aureus Isolated from Meat Carcasses and Bovine Milk in Abattoirs and Dairy Farms of the Eastern Cape, South Africa," IJERPH, MDPI, vol. 15(10), pages 1-13, October.
    3. Ishmael Festus Jaja & Ezekiel Green & Voster Muchenje, 2018. "Aerobic Mesophilic, Coliform, Escherichia coli , and Staphylococcus aureus Counts of Raw Meat from the Formal and Informal Meat Sectors in South Africa," IJERPH, MDPI, vol. 15(4), pages 1-13, April.
    4. Mokaba Shirley Malema & Akebe Luther King Abia & Roman Tandlich & Bonga Zuma & Jean-Marc Mwenge Kahinda & Eunice Ubomba-Jaswa, 2018. "Antibiotic-Resistant Pathogenic Escherichia Coli Isolated from Rooftop Rainwater-Harvesting Tanks in the Eastern Cape, South Africa," IJERPH, MDPI, vol. 15(5), pages 1-14, May.

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