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Novel Effective Yeast Strains and Their Performance in High Gravity and Very High Gravity Ethanol Fermentations from Sweet Sorghum Juice

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  • Lakkana Laopaiboon

    (Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
    Center for Alternative Energy Research and Development, Khon Kaen University, Khon Kaen 40002, Thailand)

  • Suntaree Suporn

    (Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand)

  • Preekamol Klanrit

    (Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
    Fermentation Research Center for Value Added Agricultural Products, Khon Kaen University, Khon Kaen 40002, Thailand)

  • Niphaphat Phukoetphim

    (Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand)

  • Chalida Daengbussadee

    (Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand)

  • Pattana Laopaiboon

    (Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
    Fermentation Research Center for Value Added Agricultural Products, Khon Kaen University, Khon Kaen 40002, Thailand)

Abstract

Yeasts were isolated from four potential sources, sweet sorghum juice, sugar cane juice, grapes and rambutan. The 27 yeast isolates were tested for their ethanol tolerance (15% v / v of ethanol) and ethanol fermentation performance in a synthetic ethanol production medium (200 g/L of total sugar). Only five isolates, SCJ04KKU, SCJ07KKU, SCJ09KKU, SCJ14KKU and SSJ01KKU could tolerate 15% ethanol and produce ethanol at levels higher than 55 g/L. The ethanol production efficiency from sweet sorghum juice under high gravity (HG, 200 and 240 g/L of total sugar) and very high gravity (VHG, 280 g/L of total sugar) conditions of the five isolates was tested. Saccharomyces cerevisiae NP01 and S. cerevisiae ATCC4132 were used as reference strains. The results showed that the SSJ01KKU isolate gave the highest ethanol production efficiency under all conditions. Ethanol concentration ( P E ), yield ( Y P/S ) and productivity ( Q P ) values were 98.89 g/L, 0.50 and 1.18 g/L·h, respectively, with sugar consumption ( SC ) of 98.96% under the HG condition at 200 g/L of total sugar. Under the HG condition at 240 g/L of total sugar, the P E , Y P/S and Q P values were 118.12 g/L, 0.51 and 1.41 g/L·h, respectively, with the SC of 95.79%. These values were 82.29 g/L, 0.34 and 0.98 g/L·h, respectively, with the SC of 85.59% under the VHG condition. Addition of urea into the sweet sorghum juice under all conditions significantly shortened the fermentation time, resulting in increased Q P values. Based on molecular taxonomic analysis of the five isolates using sequence analysis of the D1/D2 domain and the ITS1 and ITS2 regions, SSJ01KKU is S. cerevisiae , whereas SCJ04KKU, SCJ07KKU, SCJ09KKU and SCJ14KKU are Pichia caribbica .

Suggested Citation

  • Lakkana Laopaiboon & Suntaree Suporn & Preekamol Klanrit & Niphaphat Phukoetphim & Chalida Daengbussadee & Pattana Laopaiboon, 2021. "Novel Effective Yeast Strains and Their Performance in High Gravity and Very High Gravity Ethanol Fermentations from Sweet Sorghum Juice," Energies, MDPI, vol. 14(3), pages 1-15, January.
    • Handle: RePEc:gam:jeners:v:14:y:2021:i:3:p:557-:d:485030
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    References listed on IDEAS

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    1. Orawan Deesuth & Pattana Laopaiboon & Prasit Jaisil & Lakkana Laopaiboon, 2012. "Optimization of Nitrogen and Metal Ions Supplementation for Very High Gravity Bioethanol Fermentation from Sweet Sorghum Juice Using an Orthogonal Array Design," Energies, MDPI, vol. 5(9), pages 1-20, August.
    2. Sureerat Suwanapong & Naulchan Khongsay & Lakkana Laopaiboon & Prasit Jaisil & Pattana Laopaiboon, 2013. "Dried Spent Yeast and Its Hydrolysate as Nitrogen Supplements for Single Batch and Repeated-Batch Ethanol Fermentation from Sweet Sorghum Juice," Energies, MDPI, vol. 6(3), pages 1-14, March.
    3. Pachaya Chan-u-tit & Lakkana Laopaiboon & Prasit Jaisil & Pattana Laopaiboon, 2013. "High Level Ethanol Production by Nitrogen and Osmoprotectant Supplementation under Very High Gravity Fermentation Conditions," Energies, MDPI, vol. 6(2), pages 1-16, February.
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