IDEAS home Printed from https://ideas.repec.org/a/gam/jeners/v12y2019i13p2614-d246393.html
   My bibliography  Save this article

Assessment of the Effect of Nitrogen Concentration on Fermentation and Selection of a Highly Competitive Saccharomyces cerevisiae Strain for Efficient Ethanol Production

Author

Listed:
  • Patricia Portero Barahona

    (Centro Neotropical para Investigación de la Biomasa (CNIB), Colección de Levaduras Quito Católica (CLQCA), Pontificia Universidad Católica del Ecuador, Avda. 12 de Octubre 1076 y Roca, 17012184, Quito, Ecuador
    Agriculture and Forestry Engineering Department, ETSIIAA, Universidad de Valladolid, Avda. de Madrid 44, 34004 Palencia, Spain)

  • Jesús Martín-Gil

    (Agriculture and Forestry Engineering Department, ETSIIAA, Universidad de Valladolid, Avda. de Madrid 44, 34004 Palencia, Spain)

  • Pablo Martín-Ramos

    (Department of Agricultural and Environmental Sciences, EPS, Instituto de Investigación en Ciencias Ambientales (IUCA), University of Zaragoza, Carretera de Cuarte, s/n, 22071 Huesca, Spain)

  • Ana Briones Pérez

    (Analytical Chemistry and Food Technology Department, Universidad de Castilla-La Mancha, Avda. Camilo José Cela, 10, 13071 Ciudad Real, Spain)

  • Enrique Javier Carvajal Barriga

    (Centro Neotropical para Investigación de la Biomasa (CNIB), Colección de Levaduras Quito Católica (CLQCA), Pontificia Universidad Católica del Ecuador, Avda. 12 de Octubre 1076 y Roca, 17012184, Quito, Ecuador)

Abstract

The optimum nitrogen concentration for media supplementation and strain dominance are aspects of key importance to the industrial production of ethanol with a view to reducing costs and increasing yields. In this work, these two factors were investigated for four ethanologenic Saccharomyces cerevisiae strains (CLQCA-INT-001, CLQCA-INT-005, CLQCA-10-099, and UCLM 325), selected from the screening of 150 isolates, mostly from Ecuadorian yeast biodiversity. The effect of nitrogen concentration was assessed in terms of cellular growth, glucose consumption and ethanol production, and the yeast strains’ dominance was evaluated in continuous co-fermentation with cellular recycling by mitochondrial DNA analyses. Among the four selected yeast strains under study, CLQCA-INT-005 presented the highest glucose consumption at a nitrogen supplement concentration as low as 0.4 g·L −1 , attaining an ethanol yield of up to 96.72% in 24 h. The same yeast strain was found to be highly competitive, showing a dominance of 80% after four cycles of fermentation in co-culture. Thus, CLQCA-INT-005 may be deemed as a very promising candidate to be used both at pilot-plant scale and at industrial scale cellulosic ethanol production.

Suggested Citation

  • Patricia Portero Barahona & Jesús Martín-Gil & Pablo Martín-Ramos & Ana Briones Pérez & Enrique Javier Carvajal Barriga, 2019. "Assessment of the Effect of Nitrogen Concentration on Fermentation and Selection of a Highly Competitive Saccharomyces cerevisiae Strain for Efficient Ethanol Production," Energies, MDPI, vol. 12(13), pages 1-12, July.
    • Handle: RePEc:gam:jeners:v:12:y:2019:i:13:p:2614-:d:246393
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1996-1073/12/13/2614/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1996-1073/12/13/2614/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Sureerat Suwanapong & Naulchan Khongsay & Lakkana Laopaiboon & Prasit Jaisil & Pattana Laopaiboon, 2013. "Dried Spent Yeast and Its Hydrolysate as Nitrogen Supplements for Single Batch and Repeated-Batch Ethanol Fermentation from Sweet Sorghum Juice," Energies, MDPI, vol. 6(3), pages 1-14, March.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Lakkana Laopaiboon & Suntaree Suporn & Preekamol Klanrit & Niphaphat Phukoetphim & Chalida Daengbussadee & Pattana Laopaiboon, 2021. "Novel Effective Yeast Strains and Their Performance in High Gravity and Very High Gravity Ethanol Fermentations from Sweet Sorghum Juice," Energies, MDPI, vol. 14(3), pages 1-15, January.
    2. Puligundla, Pradeep & Smogrovicova, Daniela & Mok, Chulkyoon & Obulam, Vijaya Sarathi Reddy, 2019. "A review of recent advances in high gravity ethanol fermentation," Renewable Energy, Elsevier, vol. 133(C), pages 1366-1379.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jeners:v:12:y:2019:i:13:p:2614-:d:246393. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.