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Improvement of Bioethanol Production from Sweet Sorghum Juice under Very High Gravity Fermentation: Effect of Nitrogen, Osmoprotectant, and Aeration

Author

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  • Niphaphat Phukoetphim

    (Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand)

  • Pachaya Chan-u-tit

    (Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand)

  • Pattana Laopaiboon

    (Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
    Center for Alternative Energy Research and Development, Khon Kaen University, Khon Kaen 40002, Thailand)

  • Lakkana Laopaiboon

    (Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
    Fermentation Research Center for Value Added Agricultural Products, Khon Kaen University, Khon Kaen 40002, Thailand)

Abstract

To improve ethanol production fermentation efficiency from sweet sorghum juice under a very high gravity (VHG, 280 g/L of total sugar) condition by Saccharomyces cerevisiae NP01, dried spent yeast (DSY), yeast extract, and glycine concentrations were optimized using an L 9 (3 4 ) orthogonal array design. The results showed that the order of influence on the ethanol concentration ( P E ) was yeast extract > glycine > DSY. The optimal nutrient concentrations for ethanol production were determined as follows: yeast extract, 3; DSY, 4; and glycine, 5 g/L. When a verification experiment under the projected optimal conditions was done, the P , ethanol yield ( Y p/s ), and ethanol productivity ( Q p ) values were 120.1 g/L, 0.47, and 2.50 g/L·h, respectively. These values were similar to those of the positive control experiment with yeast extract supplementation at 9 g/L. The yeast viability under the optimal condition was higher than that of the control experiment. To improve sugar utilization and ethanol production, aeration at 2.5 vvm for 4 h was applied under the optimal nutrient supplementation. The P , Y p/s , and Q p values were significantly increased to 134.3 g/L, 0.50, and 2.80 g/L·h, respectively.

Suggested Citation

  • Niphaphat Phukoetphim & Pachaya Chan-u-tit & Pattana Laopaiboon & Lakkana Laopaiboon, 2019. "Improvement of Bioethanol Production from Sweet Sorghum Juice under Very High Gravity Fermentation: Effect of Nitrogen, Osmoprotectant, and Aeration," Energies, MDPI, vol. 12(19), pages 1-13, September.
    • Handle: RePEc:gam:jeners:v:12:y:2019:i:19:p:3620-:d:269783
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    References listed on IDEAS

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    1. Naulchan Khongsay & Lakkana Laopaiboon & Prasit Jaisil & Pattana Laopaiboon, 2012. "Optimization of Agitation and Aeration for Very High Gravity Ethanol Fermentation from Sweet Sorghum Juice by Saccharomyces cerevisiae Using an Orthogonal Array Design," Energies, MDPI, vol. 5(3), pages 1-16, February.
    2. Pachaya Chan-u-tit & Lakkana Laopaiboon & Prasit Jaisil & Pattana Laopaiboon, 2013. "High Level Ethanol Production by Nitrogen and Osmoprotectant Supplementation under Very High Gravity Fermentation Conditions," Energies, MDPI, vol. 6(2), pages 1-16, February.
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    Cited by:

    1. Hongshen Li & Hongrui Liu & Shizhong Li, 2021. "Feasibility Study on Bioethanol Production by One Phase Transition Separation Based on Advanced Solid-State Fermentation," Energies, MDPI, vol. 14(19), pages 1-14, October.

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