IDEAS home Printed from https://ideas.repec.org/a/gam/jeners/v13y2020i15p3813-d389625.html
   My bibliography  Save this article

Electro-Fenton Based Technique to Enhance Cell Harvest and Lipid Extraction from Microalgae

Author

Listed:
  • Shuai Zhang

    (Tianjin Key Laboratory of Industrial Biosystems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
    Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164-6120, USA
    These authors contributed equally to this work.)

  • Yuyong Hou

    (Tianjin Key Laboratory of Industrial Biosystems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
    These authors contributed equally to this work.)

  • Zhiyong Liu

    (Tianjin Key Laboratory of Industrial Biosystems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China)

  • Xiang Ji

    (School of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, China)

  • Di Wu

    (School of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, China)

  • Weijie Wang

    (College of Life Science, North China University of Science and Technology, Tangshan 063210, China)

  • Dongyuan Zhang

    (Tianjin Key Laboratory of Industrial Biosystems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China)

  • Wenya Wang

    (College of Life Science and Techenology, Beijing University of Chemical Technology, Beijing 100029, China)

  • Shulin Chen

    (Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164-6120, USA)

  • Fangjian Chen

    (Tianjin Key Laboratory of Industrial Biosystems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China)

Abstract

Currently, lipid extraction remains a major bottleneck in microalgae technology for biofuel production. In this study, an effective and easily controlled cell wall disruption method based on electro-Fenton reaction was used to enhance lipid extraction from the wet biomass of Nannochloropsis oceanica IMET1. The results showed that 1.27 mM of hydroxide radical (HO•) was generated under the optimal conditions with 9.1 mM FeSO 4 in a 16.4 mA·cm −2 current density for 37.0 min. After the electro-Fenton treatment, the neutral lipid extraction yield of microalgae (~155 mg) increased from 40% to 87.5%, equal to from 12.2% to 26.7% dry cell weight (DCW). In particular, the fatty acid composition remained stable. The cell wall disruption and lipid extraction processes were displayed by the transmission electron microscope (TEM) and fluorescence microscopy (FM) observations, respectively. Meanwhile, the removal efficiency of algal cells reached 85.2% within 2 h after the reaction was terminated. Furthermore, the biomass of the microalgae cultured in the electrolysis wastewater treated with fresh nutrients reached 3 g/L, which is 12-fold higher than that of the initial after 24 days. These finds provided an economic and efficient method for lipid extraction from wet microalgae, which could be easily controlled by current magnitude regulation.

Suggested Citation

  • Shuai Zhang & Yuyong Hou & Zhiyong Liu & Xiang Ji & Di Wu & Weijie Wang & Dongyuan Zhang & Wenya Wang & Shulin Chen & Fangjian Chen, 2020. "Electro-Fenton Based Technique to Enhance Cell Harvest and Lipid Extraction from Microalgae," Energies, MDPI, vol. 13(15), pages 1-14, July.
    • Handle: RePEc:gam:jeners:v:13:y:2020:i:15:p:3813-:d:389625
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1996-1073/13/15/3813/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1996-1073/13/15/3813/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Mata, Teresa M. & Martins, António A. & Caetano, Nidia. S., 2010. "Microalgae for biodiesel production and other applications: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 14(1), pages 217-232, January.
    2. Fatih Demirbas, M., 2009. "Biorefineries for biofuel upgrading: A critical review," Applied Energy, Elsevier, vol. 86(Supplemen), pages 151-161, November.
    3. Kalpesh K. Sharma & Holger Schuhmann & Peer M. Schenk, 2012. "High Lipid Induction in Microalgae for Biodiesel Production," Energies, MDPI, vol. 5(5), pages 1-22, May.
    4. Zhiyong Liu & Chenfeng Liu & Yuyong Hou & Shulin Chen & Dongguang Xiao & Juankun Zhang & Fangjian Chen, 2013. "Isolation and Characterization of a Marine Microalga for Biofuel Production with Astaxanthin as a Co-Product," Energies, MDPI, vol. 6(6), pages 1-14, May.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. José C. M. Pires & Ana L. Gonçalves, 2022. "Microalgae Cultures: Environmental Tool and Bioenergy," Energies, MDPI, vol. 15(16), pages 1-4, August.
    2. Kenichi Furuhashi & Fumio Hasegawa & Manabu Yamauchi & Yutaka Kaizu & Kenji Imou, 2020. "Improving the Energy Balance of Hydrocarbon Production Using an Inclined Solid–Liquid Separator with a Wedge-Wire Screen and Easy Hydrocarbon Recovery from Botryococcus braunii," Energies, MDPI, vol. 13(16), pages 1-14, August.
    3. Kai Ling Yu & Hwai Chyuan Ong & Halimah Badioze Zaman, 2022. "Microalgae Biomass as Biofuel and the Green Applications," Energies, MDPI, vol. 15(19), pages 1-6, October.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Behnam Tabatabai & Afua Adusei & Alok Kumar Shrivastava & Prashant Kumar Singh & Viji Sitther, 2020. "Nitrogen Deprivation in Fremyella diplosiphon Augments Lipid Production without Affecting Growth," Energies, MDPI, vol. 13(21), pages 1-12, November.
    2. Patel, Akash & Gami, Bharat & Patel, Pankaj & Patel, Beena, 2017. "Microalgae: Antiquity to era of integrated technology," Renewable and Sustainable Energy Reviews, Elsevier, vol. 71(C), pages 535-547.
    3. Kulvinder Bajwa & Narsi R Bishnoi & Anita Kirrolia, 2018. "Evaluation of Nutrient Stress (Nitrogen, Phosphorus Regimes) on Physio-Biochemical Parameters of Oleaginous Micro algal Strains and SEM Study under Nutrient Stress," International Journal of Environmental Sciences & Natural Resources, Juniper Publishers Inc., vol. 10(1), pages 01-07, April.
    4. Jha, Sunil Kr. & Bilalovic, Jasmin & Jha, Anju & Patel, Nilesh & Zhang, Han, 2017. "Renewable energy: Present research and future scope of Artificial Intelligence," Renewable and Sustainable Energy Reviews, Elsevier, vol. 77(C), pages 297-317.
    5. Sigita Vaičiulytė & Giulia Padovani & Jolanta Kostkevičienė & Pietro Carlozzi, 2014. "Batch Growth of Chlorella Vulgaris CCALA 896 versus Semi-Continuous Regimen for Enhancing Oil-Rich Biomass Productivity," Energies, MDPI, vol. 7(6), pages 1-18, June.
    6. Banerjee, Sanjukta & Banerjee, Srijoni & Ghosh, Ananta K. & Das, Debabrata, 2020. "Maneuvering the genetic and metabolic pathway for improving biofuel production in algae: Present status and future prospective," Renewable and Sustainable Energy Reviews, Elsevier, vol. 133(C).
    7. Cherng-Yuan Lin & Bo-Yu Lin, 2015. "Fatty Acid Characteristics of Isochrysis galbana Lipids Extracted Using a Microwave-Assisted Method," Energies, MDPI, vol. 8(2), pages 1-12, February.
    8. Joseph Antony Sundarsingh Tensingh & Vijayalakshmi Shankar, 2022. "Sustainable Production of Biodiesel Using UV Mutagenesis as a Strategy to Enhance the Lipid Productivity in R. mucilaginosa," Sustainability, MDPI, vol. 14(15), pages 1-15, July.
    9. Ankita Juneja & Ruben Michael Ceballos & Ganti S. Murthy, 2013. "Effects of Environmental Factors and Nutrient Availability on the Biochemical Composition of Algae for Biofuels Production: A Review," Energies, MDPI, vol. 6(9), pages 1-32, September.
    10. Fazal, Tahir & Mushtaq, Azeem & Rehman, Fahad & Ullah Khan, Asad & Rashid, Naim & Farooq, Wasif & Rehman, Muhammad Saif Ur & Xu, Jian, 2018. "Bioremediation of textile wastewater and successive biodiesel production using microalgae," Renewable and Sustainable Energy Reviews, Elsevier, vol. 82(P3), pages 3107-3126.
    11. Senem Onen Cinar & Zhi Kai Chong & Mehmet Ali Kucuker & Nils Wieczorek & Ugur Cengiz & Kerstin Kuchta, 2020. "Bioplastic Production from Microalgae: A Review," IJERPH, MDPI, vol. 17(11), pages 1-21, May.
    12. Marianela Cobos & Jae D. Paredes & J. Dylan Maddox & Gabriel Vargas-Arana & Leenin Flores & Carla P. Aguilar & Jorge L. Marapara & Juan C. Castro, 2017. "Isolation and Characterization of Native Microalgae from the Peruvian Amazon with Potential for Biodiesel Production," Energies, MDPI, vol. 10(2), pages 1-16, February.
    13. Menegazzo, Mariana Lara & Fonseca, Gustavo Graciano, 2019. "Biomass recovery and lipid extraction processes for microalgae biofuels production: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 107(C), pages 87-107.
    14. Islam, Muhammad Aminul & Heimann, Kirsten & Brown, Richard J., 2017. "Microalgae biodiesel: Current status and future needs for engine performance and emissions," Renewable and Sustainable Energy Reviews, Elsevier, vol. 79(C), pages 1160-1170.
    15. Demirbas, M. Fatih, 2011. "Biofuels from algae for sustainable development," Applied Energy, Elsevier, vol. 88(10), pages 3473-3480.
    16. Chen, Chunxiang & Ma, Xiaoqian & Liu, Kai, 2011. "Thermogravimetric analysis of microalgae combustion under different oxygen supply concentrations," Applied Energy, Elsevier, vol. 88(9), pages 3189-3196.
    17. Singh, Jasvinder & Gu, Sai, 2010. "Biomass conversion to energy in India--A critique," Renewable and Sustainable Energy Reviews, Elsevier, vol. 14(5), pages 1367-1378, June.
    18. Azad, A.K. & Rasul, M.G. & Khan, M.M.K. & Sharma, Subhash C. & Hazrat, M.A., 2015. "Prospect of biofuels as an alternative transport fuel in Australia," Renewable and Sustainable Energy Reviews, Elsevier, vol. 43(C), pages 331-351.
    19. Santhakumaran, Prasanthkumar & Kookal, Santhosh Kumar & Mathew, Linu & Ray, Joseph George, 2020. "Experimental evaluation of the culture parameters for optimum yield of lipids and other nutraceutically valuable compounds in Chloroidium saccharophillum (Kruger) comb. Nov," Renewable Energy, Elsevier, vol. 147(P1), pages 1082-1097.
    20. Aziz, Md Maniruzzaman A. & Kassim, Khairul Anuar & Shokravi, Zahra & Jakarni, Fauzan Mohd & Liu, Hong Yuan & Zaini, Nabilah & Tan, Lian See & Islam, A.B.M. Saiful & Shokravi, Hoofar, 2020. "Two-stage cultivation strategy for simultaneous increases in growth rate and lipid content of microalgae: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 119(C).

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jeners:v:13:y:2020:i:15:p:3813-:d:389625. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.