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Bioethanol production from Miscanthus using thermotolerant Saccharomyces cerevisiae mbc 2 isolated from the respiration-deficient mutants

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  • Cha, Young-Lok
  • An, Gi Hong
  • Yang, Jungwoo
  • Moon, Youn-Ho
  • Yu, Gyeong-Dan
  • Ahn, Jong-Woong

Abstract

One major obstacle of simultaneous saccharification and fermentation (SSF) is that enzymatic hydrolysis in SSF is conducted at non-optimal temperature. To break through this bottleneck, thermotolerant Saccharomyces cerevisiae strains mbc 1-4 were screened by spot-assay from respiration-deficient mutants induced by ethidium bromide. When four strains placed in liquid medium, the strains exhibited significant increased growth and fermentability at 42 °C in comparison to the host. Consequently, S. cerevisiae mbc 2 was selected to be the most suitable for bioethanol production at 42 °C. Miscanthus was pretreated with 1.5 M NaOH at 150 °C for 30 min in 1 L-vessel. As a result, 42.6% of solids containing 77.9% cellulose were recovered. Next, SSF was conducted with pretreated Miscanthus containing 3% glucan to estimate the fermentability of S. cerevisiae mbc 2 at 42 °C. In results, Ethanol concentration and theoretical ethanol yield by S. cerevisiae mbc 2 in 48 h were 15.3 g/L and 90.1%, respectively, whereas those by the control were 8.3 g/L and 49.3%, respectively. In addition, SSF with increased substrate concentration upto 9% glucan resulted in 86.3% and 82.2% of theoretical ethanol yield, respectively. Thus, our approach for SSF with high solid loading using thermotolerant S. cerevisiae mbc 2 contributes to process and microbial strain development for bioethanol production.

Suggested Citation

  • Cha, Young-Lok & An, Gi Hong & Yang, Jungwoo & Moon, Youn-Ho & Yu, Gyeong-Dan & Ahn, Jong-Woong, 2015. "Bioethanol production from Miscanthus using thermotolerant Saccharomyces cerevisiae mbc 2 isolated from the respiration-deficient mutants," Renewable Energy, Elsevier, vol. 80(C), pages 259-265.
    • Handle: RePEc:eee:renene:v:80:y:2015:i:c:p:259-265
    DOI: 10.1016/j.renene.2015.02.016
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    1. Ortiz-Muñiz, B. & Carvajal-Zarrabal, O. & Aguilar, B. & Aguilar-Uscanga, M.G., 2012. "Improvement in ethanol production using respiratory deficient phenotype of a wild type yeast Saccharomyces cerevisiae ITV-01," Renewable Energy, Elsevier, vol. 37(1), pages 197-201.
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    Cited by:

    1. Jahnavi, Gentela & Prashanthi, Govumoni Sai & Sravanthi, Koti & Rao, Linga Venkateswar, 2017. "Status of availability of lignocellulosic feed stocks in India: Biotechnological strategies involved in the production of Bioethanol," Renewable and Sustainable Energy Reviews, Elsevier, vol. 73(C), pages 798-820.
    2. Romaní, Aloia & Ruiz, Héctor A. & Teixeira, José A. & Domingues, Lucília, 2016. "Valorization of Eucalyptus wood by glycerol-organosolv pretreatment within the biorefinery concept: An integrated and intensified approach," Renewable Energy, Elsevier, vol. 95(C), pages 1-9.
    3. Arora, Richa & Behera, Shuvashish & Kumar, Sachin, 2015. "Bioprospecting thermophilic/thermotolerant microbes for production of lignocellulosic ethanol: A future perspective," Renewable and Sustainable Energy Reviews, Elsevier, vol. 51(C), pages 699-717.

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