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Directly convert lignocellulosic biomass to H2 without pretreatment and added cellulase by two-stage fermentation in semi-continuous modes

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  • Hu, Jun
  • Cao, Wen
  • Guo, Liejin

Abstract

Cost-competitive production of lignocellulosic H2 using biological methods is limited by energy-intensive pretreatments and expensive cellulase hydrolysis. Here we report stable two-stage fermentation to convert unpretreated energy sorghum without added cellulase to H2, where Clostridium thermocellum was fed semi-continuously with 20 g/L sorghum in the first stage and then Rhodobacter sphaeroides consumed the effluent wastes in the second stage to achieve residence times (RTs) of 60, 48, and 24 h. Glutamic acid was the preferred nitrogen source for coordinating the two-stage, two-organism fermentation. 25.0 ± 1.1 mL-H2/day in consolidated bioprocessing stage and 133.0 ± 1.5 mL-H2/day in photofermentation stage were obtained for 200 mL cultures at RT 48 h. Propionate is the main product accumulated, with cellobiose, xylose, arabinose, formate, acetate and ethanol also observed in the effluent wastes, which were degraded effectively by Rhodobacter sphaeroides with COD removal of 72.6%. These results provide references for developing low-cost H2 production from lignocellulose.

Suggested Citation

  • Hu, Jun & Cao, Wen & Guo, Liejin, 2021. "Directly convert lignocellulosic biomass to H2 without pretreatment and added cellulase by two-stage fermentation in semi-continuous modes," Renewable Energy, Elsevier, vol. 170(C), pages 866-874.
  • Handle: RePEc:eee:renene:v:170:y:2021:i:c:p:866-874
    DOI: 10.1016/j.renene.2021.02.062
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    Cited by:

    1. Gomes, Michelle Garcia & Paranhos, Aline Gomes de Oliveira & Camargos, Adonai Bruneli & Baêta, Bruno Eduardo Lobo & Baffi, Milla Alves & Gurgel, Leandro Vinícius Alves & Pasquini, Daniel, 2022. "Pretreatment of sugarcane bagasse with dilute citric acid and enzymatic hydrolysis: Use of black liquor and solid fraction for biogas production," Renewable Energy, Elsevier, vol. 191(C), pages 428-438.
    2. Kavitha, S. & Gajendran, T. & Saranya, K. & Selvakumar, P. & Manivasagan, V. & Jeevitha, S., 2022. "An insight - A statistical investigation of consolidated bioprocessing of Allium ascalonicum leaves to ethanol using Hangateiclostridium thermocellum KSMK1203 and synthetic consortium," Renewable Energy, Elsevier, vol. 187(C), pages 403-416.
    3. Fakayode, Olugbenga Abiola & Akpabli-Tsigbe, Nelson Dzidzorgbe Kwaku & Wahia, Hafida & Tu, Shanshan & Ren, Manni & Zhou, Cunshan & Ma, Haile, 2021. "Integrated bioprocess for bio-ethanol production from watermelon rind biomass: Ultrasound-assisted deep eutectic solvent pretreatment, enzymatic hydrolysis and fermentation," Renewable Energy, Elsevier, vol. 180(C), pages 258-270.

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