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Sugarcane bagasse saccharification using Aspergillus tubingensis enzymatic cocktail for 2G bio-ethanol production

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  • Prajapati, Bhanu Pratap
  • Jana, Uttam Kumar
  • Suryawanshi, Rahul Kumar
  • Kango, Naveen

Abstract

In order to meet the increasing demand of 2G ethanol, sugarcane bagasse (SCB), a low cost by-product of sugarcane industry, can be utilized. However, cost-effective degradation of SCB into constituent fermentable sugars and subsequent ethanolic fermentation remains to be a far-fetched goal. In the present study, cellulase and hemicellulase cocktail produced by Aspergillus tubingensis NKBP-55 was applied for SCB hydrolysis under parametrically optimized conditions. Enzyme treatment (SCB: 7%, enzyme load: 1U = 1 mg) resulted in the liberation of fermentable mono-sugars 20 mg/mL (glucose, xylose and arabinose). Powder X-ray diffraction (PXRD) analysis revealed increase in the crystallinity index from 58.4 to 63.9% as a result of degradation of hemicellulose and amorphous cellulose. SCB hydrolysate was fermented to ethanol (0.415 g/g) by glucose and xylose-fermenting yeast, Candida shehatae NCIM 3501. Maximum ethanol concentration (15.54 ± 0.3 g/L) with 77.9% fermentation efficiency and 0.161 g/L/h productivity was achieved. The present study revealed that A. tubingensis enzyme cocktail can be used for efficient SCB hydrolysis and conversion of resulting hydrolysate into bioethanol using C. shehatae.

Suggested Citation

  • Prajapati, Bhanu Pratap & Jana, Uttam Kumar & Suryawanshi, Rahul Kumar & Kango, Naveen, 2020. "Sugarcane bagasse saccharification using Aspergillus tubingensis enzymatic cocktail for 2G bio-ethanol production," Renewable Energy, Elsevier, vol. 152(C), pages 653-663.
    • Handle: RePEc:eee:renene:v:152:y:2020:i:c:p:653-663
    DOI: 10.1016/j.renene.2020.01.063
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    References listed on IDEAS

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    1. Brienzo, Michel & Fikizolo, Simphiwe & Benjamin, Yuda & Tyhoda, Luvuyo & Görgens, Johann, 2017. "Influence of pretreatment severity on structural changes, lignin content and enzymatic hydrolysis of sugarcane bagasse samples," Renewable Energy, Elsevier, vol. 104(C), pages 271-280.
    2. Patel, Harshvadan & Chapla, Digantkumar & Shah, Amita, 2017. "Bioconversion of pretreated sugarcane bagasse using enzymatic and acid followed by enzymatic hydrolysis approaches for bioethanol production," Renewable Energy, Elsevier, vol. 109(C), pages 323-331.
    3. Jain, Lavika & Agrawal, Deepti, 2018. "Performance evaluation of fungal cellulases with dilute acid pretreated sugarcane bagasse: A robust bioprospecting strategy for biofuel enzymes," Renewable Energy, Elsevier, vol. 115(C), pages 978-988.
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    Cited by:

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    2. Srivastava, Neha & Singh, Preeti & Srivastava, Manish & Lal, Basant & Singh, Rajeev & Ahmad, Irfan & Gupta, Vijai Kumar, 2024. "A review on the scope and challenges of Saccharum spontaneum waste in the context of lignocellulosic biomass for sustainable bioenergy applications," Renewable and Sustainable Energy Reviews, Elsevier, vol. 199(C).
    3. Vandenberghe, L.P.S. & Valladares-Diestra, K.K. & Bittencourt, G.A. & Zevallos Torres, L.A. & Vieira, S. & Karp, S.G. & Sydney, E.B. & de Carvalho, J.C. & Thomaz Soccol, V. & Soccol, C.R., 2022. "Beyond sugar and ethanol: The future of sugarcane biorefineries in Brazil," Renewable and Sustainable Energy Reviews, Elsevier, vol. 167(C).
    4. Sattar Jabbar Murad Algayyim & Talal Yusaf & Naseer H. Hamza & Andrew P. Wandel & I. M. Rizwanul Fattah & Mohamd Laimon & S. M. Ashrafur Rahman, 2022. "Sugarcane Biomass as a Source of Biofuel for Internal Combustion Engines (Ethanol and Acetone-Butanol-Ethanol): A Review of Economic Challenges," Energies, MDPI, vol. 15(22), pages 1-17, November.

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