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Saccharification of kans grass biomass by a novel fractional hydrolysis method followed by co-culture fermentation for bioethanol production

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  • Mishra, Archana
  • Ghosh, Sanjoy

Abstract

Present work is focused mainly on recovering maximum amount of soluble pentose and hexose sugars separately, direct from the kans grass (lignocellulosic biomass) by a novel fractional hydrolysis process (pretreatment + hydrolysis + detoxification) with minimum toxic products generation. Effect of biomass loading and preheating time on the fractional hydrolysis process was studied along with various strategies up to 30% H2SO4 (v/v) to reduce the number of stages for acid use minimisation. 84.88% of total reducing sugar present in the kans grass biomass was extracted as separate pentose and hexose sugar fractions with negligible toxics. Zymomonas mobilis and Scheffersomyces shehatae were used during co-culture fermentation; 92.13% of the sugar present in xylose-rich fraction (initial sugar: 21.87 g/L) and 96.32% of glucose-rich fraction (initial sugar: 40.32 g/L) were utilised to produce 25.0 g/L ethanol from the kans grass biomass hydrolysate; thereby achieving 78.6% of the maximum theoretical ethanol production with an average yield of 0.435.

Suggested Citation

  • Mishra, Archana & Ghosh, Sanjoy, 2020. "Saccharification of kans grass biomass by a novel fractional hydrolysis method followed by co-culture fermentation for bioethanol production," Renewable Energy, Elsevier, vol. 146(C), pages 750-759.
    • Handle: RePEc:eee:renene:v:146:y:2020:i:c:p:750-759
    DOI: 10.1016/j.renene.2019.07.016
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    References listed on IDEAS

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    1. Kim, Seonghun & Kim, Chul Ho, 2014. "Evaluation of whole Jerusalem artichoke (Helianthus tuberosus L.) for consolidated bioprocessing ethanol production," Renewable Energy, Elsevier, vol. 65(C), pages 83-91.
    2. Demirbas, Ayhan, 2009. "Political, economic and environmental impacts of biofuels: A review," Applied Energy, Elsevier, vol. 86(Supplemen), pages 108-117, November.
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    1. Liao, Junwei & Zhong, Quanwang & Gu, Juwen & Qiu, Songbai & Meng, Qingwei & Zhang, Qian & Wang, Tiejun, 2022. "New approach for bio-jet fuels production by hydrodeoxygenation of higher alcohols derived from C-C coupling of bio-ethanol," Applied Energy, Elsevier, vol. 324(C).

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