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Saccharification of kans grass biomass by a novel fractional hydrolysis method followed by co-culture fermentation for bioethanol production

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  • Mishra, Archana
  • Ghosh, Sanjoy

Abstract

Present work is focused mainly on recovering maximum amount of soluble pentose and hexose sugars separately, direct from the kans grass (lignocellulosic biomass) by a novel fractional hydrolysis process (pretreatment + hydrolysis + detoxification) with minimum toxic products generation. Effect of biomass loading and preheating time on the fractional hydrolysis process was studied along with various strategies up to 30% H2SO4 (v/v) to reduce the number of stages for acid use minimisation. 84.88% of total reducing sugar present in the kans grass biomass was extracted as separate pentose and hexose sugar fractions with negligible toxics. Zymomonas mobilis and Scheffersomyces shehatae were used during co-culture fermentation; 92.13% of the sugar present in xylose-rich fraction (initial sugar: 21.87 g/L) and 96.32% of glucose-rich fraction (initial sugar: 40.32 g/L) were utilised to produce 25.0 g/L ethanol from the kans grass biomass hydrolysate; thereby achieving 78.6% of the maximum theoretical ethanol production with an average yield of 0.435.

Suggested Citation

  • Mishra, Archana & Ghosh, Sanjoy, 2020. "Saccharification of kans grass biomass by a novel fractional hydrolysis method followed by co-culture fermentation for bioethanol production," Renewable Energy, Elsevier, vol. 146(C), pages 750-759.
    • Handle: RePEc:eee:renene:v:146:y:2020:i:c:p:750-759
    DOI: 10.1016/j.renene.2019.07.016
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    References listed on IDEAS

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    1. Demirbas, Ayhan, 2009. "Political, economic and environmental impacts of biofuels: A review," Applied Energy, Elsevier, vol. 86(Supplemen), pages 108-117, November.
    2. Kim, Seonghun & Kim, Chul Ho, 2014. "Evaluation of whole Jerusalem artichoke (Helianthus tuberosus L.) for consolidated bioprocessing ethanol production," Renewable Energy, Elsevier, vol. 65(C), pages 83-91.
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    Cited by:

    1. Srivastava, Neha & Singh, Preeti & Srivastava, Manish & Lal, Basant & Singh, Rajeev & Ahmad, Irfan & Gupta, Vijai Kumar, 2024. "A review on the scope and challenges of Saccharum spontaneum waste in the context of lignocellulosic biomass for sustainable bioenergy applications," Renewable and Sustainable Energy Reviews, Elsevier, vol. 199(C).
    2. Liao, Junwei & Zhong, Quanwang & Gu, Juwen & Qiu, Songbai & Meng, Qingwei & Zhang, Qian & Wang, Tiejun, 2022. "New approach for bio-jet fuels production by hydrodeoxygenation of higher alcohols derived from C-C coupling of bio-ethanol," Applied Energy, Elsevier, vol. 324(C).

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