IDEAS home Printed from https://ideas.repec.org/a/eee/appene/v208y2017icp1420-1429.html
   My bibliography  Save this article

Improved bioethanol production from corn stover: Role of enzymes, inducers and simultaneous product recovery

Author

Listed:
  • Dhiman, Saurabh Sudha
  • David, Aditi
  • Braband, Vanessa W.
  • Hussein, Abdulmenan
  • Salem, David R.
  • Sani, Rajesh K.

Abstract

Two different endoxylanase genes of deep biosphere bacteria were cloned and overexpressed in E. coli. Overexpression resulted in 11- and 8-fold improvement in endoxylanase activity of XylSG7 and XylSG13 proteins, respectively. Purified XylSG7 and XylSG13 showed an optimal catalytic temperature of 60°C and 65°C, respectively, with a half-life of 20h under similar operational conditions (60°C, pH 7.0). The KM value for XylSG7 was 0.2371mgmL−1 compared to 0.4768mgmL−1 exhibited by XylSG13 with beechwood xylan. Evaluation of surface characteristics of endoxylanases through Surface Plasmon Resonance highlights a novel approach to characterization of binding prior to covalent immobilization. For improved hydrolysis of corn stover (CS), two enzymatic cocktails were prepared by mixing immobilized XylSG7 and XylSG13 with Cellic® C-Tec2, separately. Application of purified immobilized endoxylanases for CS hydrolysis is a pioneering effort in bioethanol production. Both immobilized endoxylanase were successfully reused up to 4 hydrolysis cycles with a fresh supplement of Cellic® C-Tec2 each time. Kluyveromyces marxianus yeast was selected for its thermotolerant properties, weak glucose repression and ability to metabolize the hemicellulolytic hydrolyzate. A novel high-temperature-high-pressure (HTHP) technique was deployed for maximal utilization of sugars, and enhanced recovery of the produced ethanol, during fermentation. Intermittent use of HTHP simultaneously with the fermentation reaction resulted in 18.2% improved ethanol production over the conventional fermentation process. Simultaneous recovery of ethanol prompted the complete utilization of reducing sugars, compared to a residual concentration of 11.2g/L observed with a conventional process. These findings are the first to be reported on the application of the HTHP technique for improved ethanol production, and on a highly thermostable endoxylanase showing the lowest KM value to date.

Suggested Citation

  • Dhiman, Saurabh Sudha & David, Aditi & Braband, Vanessa W. & Hussein, Abdulmenan & Salem, David R. & Sani, Rajesh K., 2017. "Improved bioethanol production from corn stover: Role of enzymes, inducers and simultaneous product recovery," Applied Energy, Elsevier, vol. 208(C), pages 1420-1429.
    • Handle: RePEc:eee:appene:v:208:y:2017:i:c:p:1420-1429
    DOI: 10.1016/j.apenergy.2017.09.013
    as

    Download full text from publisher

    File URL: http://www.sciencedirect.com/science/article/pii/S030626191731303X
    Download Restriction: Full text for ScienceDirect subscribers only
    File URL: https://libkey.io/10.1016/j.apenergy.2017.09.013?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. Wang, Yu & Ebadian, Mahmood & Sokhansanj, Shahab & Webb, Erin & Lau, Anthony, 2017. "Impact of the biorefinery size on the logistics of corn stover supply – A scenario analysis," Applied Energy, Elsevier, vol. 198(C), pages 360-376.
    2. Fatih Demirbas, M., 2009. "Biorefineries for biofuel upgrading: A critical review," Applied Energy, Elsevier, vol. 86(Supplemen), pages 151-161, November.
    3. Xu, Zhiheng & Liu, Yucheng & Williams, Isaiah & Li, Yan & Qian, Fengyu & Wang, Lei & Lei, Yu & Li, Baikun, 2017. "Flat enzyme-based lactate biofuel cell integrated with power management system: Towards long term in situ power supply for wearable sensors," Applied Energy, Elsevier, vol. 194(C), pages 71-80.
    4. Tran, Dang-Thuan & Chen, Ching-Lung & Chang, Jo-Shu, 2016. "Continuous biodiesel conversion via enzymatic transesterification catalyzed by immobilized Burkholderia lipase in a packed-bed bioreactor," Applied Energy, Elsevier, vol. 168(C), pages 340-350.
    5. Cheng, Lingfeng & Anderson, C. Lindsay, 2016. "Financial sustainability for a lignocellulosic biorefinery under carbon constraints and price downside risk," Applied Energy, Elsevier, vol. 177(C), pages 98-107.
    6. Budzianowski, Wojciech M. & Postawa, Karol, 2016. "Total Chain Integration of sustainable biorefinery systems," Applied Energy, Elsevier, vol. 184(C), pages 1432-1446.
    7. Zabed, H. & Sahu, J.N. & Suely, A. & Boyce, A.N. & Faruq, G., 2017. "Bioethanol production from renewable sources: Current perspectives and technological progress," Renewable and Sustainable Energy Reviews, Elsevier, vol. 71(C), pages 475-501.
    8. Gerald W. Hsu & Matthias Ober & Thomas Carell & Lorena S. Beese, 2004. "Error-prone replication of oxidatively damaged DNA by a high-fidelity DNA polymerase," Nature, Nature, vol. 431(7005), pages 217-221, September.
    9. Xu, Youjie & Wang, Donghai, 2017. "Integrating starchy substrate into cellulosic ethanol production to boost ethanol titers and yields," Applied Energy, Elsevier, vol. 195(C), pages 196-203.
    10. Jin, Wenxiang & Chen, Ling & Hu, Meng & Sun, Dan & Li, Ao & Li, Ying & Hu, Zhen & Zhou, Shiguang & Tu, Yuanyuan & Xia, Tao & Wang, Yanting & Xie, Guosheng & Li, Yanbin & Bai, Baowei & Peng, Liangcai, 2016. "Tween-80 is effective for enhancing steam-exploded biomass enzymatic saccharification and ethanol production by specifically lessening cellulase absorption with lignin in common reed," Applied Energy, Elsevier, vol. 175(C), pages 82-90.
    11. Li, Tian & Zhou, Lean & Qian, Yawei & Wan, Lili & Du, Qing & Li, Nan & Wang, Xin, 2017. "Gravity settling of planktonic bacteria to anodes enhances current production of microbial fuel cells," Applied Energy, Elsevier, vol. 198(C), pages 261-266.
    12. Rastogi, Meenal & Shrivastava, Smriti, 2017. "Recent advances in second generation bioethanol production: An insight to pretreatment, saccharification and fermentation processes," Renewable and Sustainable Energy Reviews, Elsevier, vol. 80(C), pages 330-340.
    13. Yu, Menghui & Li, Jihong & Li, Shizhong & Du, Ran & Jiang, Yan & Fan, Guifang & Zhao, Gang & Chang, Sandra, 2014. "A cost-effective integrated process to convert solid-state fermented sweet sorghum bagasse into cellulosic ethanol," Applied Energy, Elsevier, vol. 115(C), pages 331-336.
    14. Mesa, Leyanis & Martínez, Yenisleidy & Barrio, Edenny & González, Erenio, 2017. "Desirability function for optimization of Dilute Acid pretreatment of sugarcane straw for ethanol production and preliminary economic analysis based in three fermentation configurations," Applied Energy, Elsevier, vol. 198(C), pages 299-311.
    15. Frankó, Balázs & Galbe, Mats & Wallberg, Ola, 2016. "Bioethanol production from forestry residues: A comparative techno-economic analysis," Applied Energy, Elsevier, vol. 184(C), pages 727-736.
    16. Zhu, Shengdong & Luo, Fang & Huang, Wenjing & Huang, Wangxiang & Wu, Yuanxin, 2017. "Comparison of three fermentation strategies for alleviating the negative effect of the ionic liquid 1-ethyl-3-methylimidazolium acetate on lignocellulosic ethanol production," Applied Energy, Elsevier, vol. 197(C), pages 124-131.
    17. Haro, P. & Ollero, P. & Villanueva Perales, A.L. & Gómez-Barea, A., 2013. "Thermochemical biorefinery based on dimethyl ether as intermediate: Technoeconomic assessment," Applied Energy, Elsevier, vol. 102(C), pages 950-961.
    18. Muruaga, María Laura & Carvalho, Kátia G. & Domínguez, José Manuel & de Souza Oliveira, Ricardo Pinheiro & Perotti, Nora, 2016. "Isolation and characterization of Saccharomyces species for bioethanol production from sugarcane molasses: Studies of scale up in bioreactor," Renewable Energy, Elsevier, vol. 85(C), pages 649-656.
    19. Verma, Puneet & Sharma, M.P., 2016. "Review of process parameters for biodiesel production from different feedstocks," Renewable and Sustainable Energy Reviews, Elsevier, vol. 62(C), pages 1063-1071.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Adamu, Haruna & Bello, Usman & Yuguda, Abubakar Umar & Tafida, Usman Ibrahim & Jalam, Abdullahi Mohammad & Sabo, Ahmed & Qamar, Mohammad, 2023. "Production processes, techno-economic and policy challenges of bioenergy production from fruit and vegetable wastes," Renewable and Sustainable Energy Reviews, Elsevier, vol. 186(C).

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Kuo, Yen-Ting & Chen, Ju-Shiou & Yang, Tzu-Yueh & Wan, Hou-Peng, 2018. "Technical and Economic approach of bioethanol production from nanofiltration of biomass chemical hydrolysis solutions," Applied Energy, Elsevier, vol. 215(C), pages 426-436.
    2. Cheng, F. & Brewer, C.E., 2021. "Conversion of protein-rich lignocellulosic wastes to bio-energy: Review and recommendations for hydrolysis + fermentation and anaerobic digestion," Renewable and Sustainable Energy Reviews, Elsevier, vol. 146(C).
    3. Mesa, Leyanis & Martínez, Yenisleidy & Celia de Armas, Ana & González, Erenio, 2020. "Ethanol production from sugarcane straw using different configurations of fermentation and techno-economical evaluation of the best schemes," Renewable Energy, Elsevier, vol. 156(C), pages 377-388.
    4. Clauser, Nicolás M. & Felissia, Fernando E. & Area, María C. & Vallejos, María E., 2021. "A framework for the design and analysis of integrated multi-product biorefineries from agricultural and forestry wastes," Renewable and Sustainable Energy Reviews, Elsevier, vol. 139(C).
    5. Holmatov, B. & Schyns, J.F. & Krol, M.S. & Gerbens-Leenes, P.W. & Hoekstra, A.Y., 2021. "Can crop residues provide fuel for future transport? Limited global residue bioethanol potentials and large associated land, water and carbon footprints," Renewable and Sustainable Energy Reviews, Elsevier, vol. 149(C).
    6. Jain, Sanyam & Kumar, Shushil, 2024. "A comprehensive review of bioethanol production from diverse feedstocks: Current advancements and economic perspectives," Energy, Elsevier, vol. 296(C).
    7. Ge, Yuntian & Li, Lin, 2018. "System-level energy consumption modeling and optimization for cellulosic biofuel production," Applied Energy, Elsevier, vol. 226(C), pages 935-946.
    8. Alizadeh, Reza & Lund, Peter D. & Soltanisehat, Leili, 2020. "Outlook on biofuels in future studies: A systematic literature review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 134(C).
    9. Zabed, Hossain M. & Akter, Suely & Yun, Junhua & Zhang, Guoyan & Awad, Faisal N. & Qi, Xianghui & Sahu, J.N., 2019. "Recent advances in biological pretreatment of microalgae and lignocellulosic biomass for biofuel production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 105(C), pages 105-128.
    10. Eckert, C.T. & Frigo, E.P. & Albrecht, L.P. & Albrecht, A.J.P. & Christ, D. & Santos, W.G. & Berkembrock, E. & Egewarth, V.A., 2018. "Maize ethanol production in Brazil: Characteristics and perspectives," Renewable and Sustainable Energy Reviews, Elsevier, vol. 82(P3), pages 3907-3912.
    11. Ishtiaq Ahmed & Muhammad Anjum Zia & Huma Afzal & Shaheez Ahmed & Muhammad Ahmad & Zain Akram & Farooq Sher & Hafiz M. N. Iqbal, 2021. "Socio-Economic and Environmental Impacts of Biomass Valorisation: A Strategic Drive for Sustainable Bioeconomy," Sustainability, MDPI, vol. 13(8), pages 1-32, April.
    12. Carrillo-Nieves, Danay & Rostro Alanís, Magdalena J. & de la Cruz Quiroz, Reynaldo & Ruiz, Héctor A. & Iqbal, Hafiz M.N. & Parra-Saldívar, Roberto, 2019. "Current status and future trends of bioethanol production from agro-industrial wastes in Mexico," Renewable and Sustainable Energy Reviews, Elsevier, vol. 102(C), pages 63-74.
    13. Perkins, Greg & Bhaskar, Thallada & Konarova, Muxina, 2018. "Process development status of fast pyrolysis technologies for the manufacture of renewable transport fuels from biomass," Renewable and Sustainable Energy Reviews, Elsevier, vol. 90(C), pages 292-315.
    14. di Bitonto, Luigi & Reynel-Ávila, Hilda Elizabeth & Mendoza-Castillo, Didilia Ileana & Bonilla-Petriciolet, Adrián & Durán-Valle, Carlos J. & Pastore, Carlo, 2020. "Synthesis and characterization of nanostructured calcium oxides supported onto biochar and their application as catalysts for biodiesel production," Renewable Energy, Elsevier, vol. 160(C), pages 52-66.
    15. Omojola Awogbemi & Daramy Vandi Von Kallon & Emmanuel Idoko Onuh & Victor Sunday Aigbodion, 2021. "An Overview of the Classification, Production and Utilization of Biofuels for Internal Combustion Engine Applications," Energies, MDPI, vol. 14(18), pages 1-43, September.
    16. Gupte, Ameya Pankaj & Basaglia, Marina & Casella, Sergio & Favaro, Lorenzo, 2022. "Rice waste streams as a promising source of biofuels: feedstocks, biotechnologies and future perspectives," Renewable and Sustainable Energy Reviews, Elsevier, vol. 167(C).
    17. Melendez, Jesus R. & Mátyás, Bence & Hena, Sufia & Lowy, Daniel A. & El Salous, Ahmed, 2022. "Perspectives in the production of bioethanol: A review of sustainable methods, technologies, and bioprocesses," Renewable and Sustainable Energy Reviews, Elsevier, vol. 160(C).
    18. Man, Hanyang & Liu, Huan & Xiao, Qian & Deng, Fanyuan & Yu, Qiao & Wang, Kai & Yang, Zhengjun & Wu, Ye & He, Kebin & Hao, Jiming, 2018. "How ethanol and gasoline formula changes evaporative emissions of the vehicles," Applied Energy, Elsevier, vol. 222(C), pages 584-594.
    19. Aboagye, D. & Banadda, N. & Kiggundu, N. & Kabenge, I., 2017. "Assessment of orange peel waste availability in ghana and potential bio-oil yield using fast pyrolysis," Renewable and Sustainable Energy Reviews, Elsevier, vol. 70(C), pages 814-821.
    20. Bharathiraja, B. & Jayamuthunagai, J. & Sudharsanaa, T. & Bharghavi, A. & Praveenkumar, R. & Chakravarthy, M. & Yuvaraj, D., 2017. "Biobutanol – An impending biofuel for future: A review on upstream and downstream processing tecniques," Renewable and Sustainable Energy Reviews, Elsevier, vol. 68(P1), pages 788-807.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:eee:appene:v:208:y:2017:i:c:p:1420-1429. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Catherine Liu (email available below). General contact details of provider: http://www.elsevier.com/wps/find/journaldescription.cws_home/405891/description#description .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.