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Examination of Mycobacterium avium subsp. avium distribution in naturally infected hens by culture and triplex quantitative real time PCR

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Listed:
  • M. Kaevska

    (Veterinary Research Institute, Brno, Czech Republic)

  • I. Slana

    (Veterinary Research Institute, Brno, Czech Republic)

  • P. Kralik

    (Veterinary Research Institute, Brno, Czech Republic)

  • I. Pavlik

    (Veterinary Research Institute, Brno, Czech Republic)

Abstract

Mycobacterium avium subsp. avium (MAA) is the etiologic agent of avian tuberculosis, a chronic contagious disease described in a wide variety of domestic and wild bird species. The aims of this study were to assess the advantages of triplex quantitative real time PCR (qPCR) in comparison with culture testing for distribution of MAA in the organs of hens displaying varying degrees of clinical symptoms of the disease. From one small flock of ten hens and one cock with a history of weight loss, 98 tissue samples were examined in total. Pathological lesions were observed in six hens from which two were clinically ill. A total of 12 samples were positive by culture and 16 were positive by IS901 and IS1245 qPCR, confirming MAA infection. In conclusion, qPCR was a faster and more reliable alternative method in comparison with conventional culture analysis. Due to the detection of MAA in the muscle tissue of one hen, consumption of under cooked meat originating from infected fowl could pose a threat to immunosuppressed individuals.

Suggested Citation

  • M. Kaevska & I. Slana & P. Kralik & I. Pavlik, 2010. "Examination of Mycobacterium avium subsp. avium distribution in naturally infected hens by culture and triplex quantitative real time PCR," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 55(7), pages 325-330.
    • Handle: RePEc:caa:jnlvet:v:55:y:2010:i:7:id:2966-vetmed
    DOI: 10.17221/2966-VETMED
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    References listed on IDEAS

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    1. I. Pavlik & P. Jahn & M. Moravkova & L. Matlova & F. Treml & A. Cizek & E. Nesnalova & L. Dvorska-Bartosova & R. Halouzka, 2008. "Lung tuberculosis in a horse caused by Mycobacterium avium subsp. avium of serotype 2: a case report," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 53(2), pages 111-116.
    2. M. Pate & M. Moravkova & B. Krt & I. Pavlik & M. Ocepek, 2009. "Genotyping of Mycobacterium avium subsp. avium isolates from domestic animals in Slovenia by IS901 RFLP," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 54(6), pages 270-279.
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    Cited by:

    1. Petr Králík & Hana Přikrylová Vondrušková & Iva Slaná & Monika Morávková & Ivo Pavlík, 2011. "Survival of Mycobacterium avium subsp. hominissuis in homemade smoked pork sausages," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 29(6), pages 647-653.

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