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Risk assessment of mycobacterial infections (human tuberculosis and avian mycobacteriosis) during anatomical dissection of cadavers

Author

Listed:
  • M. Bartos

    (Veterinary Research Institute, Brno, Czech Republic)

  • H. Pavlikova

    (Masaryk University, Brno, Czech Republic
    Veterinary and Pharmaceutical University, Brno, Czech Republic)

  • L. Dvorska

    (Veterinary Research Institute, Brno, Czech Republic)

  • R. Horvath

    (Genex CZ, Ltd., Brno, Czech Republic)

  • M. Dendis

    (Genex CZ, Ltd., Brno, Czech Republic)

  • P. Flodr

    (Palacky University, Olomouc, Czech Republic)

  • Z. Kolar

    (Palacky University, Olomouc, Czech Republic)

  • R.T. Weston

    (Veterinary Research Institute, Brno, Czech Republic
    LaTrobe University, Melbourne, Australia)

  • L. Pac

    (Masaryk University, Brno, Czech Republic)

  • L. Matlova

    (Veterinary Research Institute, Brno, Czech Republic)

  • I. Pavlik

    (Veterinary Research Institute, Brno, Czech Republic)

Abstract

The aim of this work was to study the presence of mycobacteria in tissue samples from four cadavers fixed with formalin, and tissue samples from a recently deceased unpreserved individual, who had a history of human tuberculosis infection, undergoing a post mortem (cause of death not related to tuberculosis). All were examined for the presence of tuberculous lesions and the specific presence of Mycobacterium tuberculosis complex (MTC) and M. avium complex (MAC) members by microscopy, culture, and PCR analysis of four genomic elements (IS6110, mtp40, IS901, and IS1245). Microscopy examination after the Ziehl-Neelsen staining and culture examination for the presence of mycobacteria were negative in all 22 tissue samples from the four embalmed cadavers. PCR analysis of IS6110 and mtp40 was positive in tissue samples of tuberculous lesions from the lungs of two embalmed cadavers, and from intact kidney tissue of one of these cadavers. Microscopy and culture examinations of liver and spleen tissues from the unpreserved cadaver were positive for mycobacteria. PCR analysis, specific for M. avium subsp. avium, was positive in both tissue samples with, and without tuberculous lesions.

Suggested Citation

  • M. Bartos & H. Pavlikova & L. Dvorska & R. Horvath & M. Dendis & P. Flodr & Z. Kolar & R.T. Weston & L. Pac & L. Matlova & I. Pavlik, 2006. "Risk assessment of mycobacterial infections (human tuberculosis and avian mycobacteriosis) during anatomical dissection of cadavers," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 51(5), pages 311-319.
    • Handle: RePEc:caa:jnlvet:v:51:y:2006:i:5:id:5551-vetmed
    DOI: 10.17221/5551-VETMED
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    References listed on IDEAS

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    1. L. Dvorská & M. Bartoš & G. Martin & W. Erler & I. Pavlík, 2001. "Strategies for differentiation, identification and typing of medically important species of mycobacteria by molecular methods," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 46(11-12), pages 309-328.
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    Cited by:

    1. J.E. Shitaye & B. Getahun & T. Alemayehu & M. Skoric & F. Treml & P. Fictum & V. Vrbas & I. Pavlik, 2006. "A prevalence study of bovine tuberculosis by using abattoir meat inspection and tuberculin skin testing data, histopathological and IS6110 PCR examination of tissues with tuberculous lesions in cattle," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 51(11), pages 512-522.

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