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Strategies for differentiation, identification and typing of medically important species of mycobacteria by molecular methods

Author

Listed:
  • L. Dvorská

    (Veterinary Research Institute, Brno, Czech Republic)

  • M. Bartoš

    (Veterinary Research Institute, Brno, Czech Republic)

  • G. Martin

    (Veterinary Research Institute, Brno, Czech Republic)

  • W. Erler

    (Veterinary Research Institute, Brno, Czech Republic)

  • I. Pavlík

    (Veterinary Research Institute, Brno, Czech Republic)

Abstract

Molecular biology methods offer new opportunities to differentiate, identify and type bacterial species and strains. These methods use the variability of nucleic sequences of genes such as 16S rDNA, beta subunit RNA-ase (rpoB), gyrase (gyrB), rDNA internal transcribed spacer and other genes. The aim of this paper is to provide comprehensive information about the methods available to differentiate and identify species of mycobacteria at the DNA sequence level. The methods discussed in the review include PCR, PCR-REA, sequencing analysis, spoligotyping and DNA fingerprinting. These methods have been applied to both the "universal" part of the genome and to specific mycobacterial genes.

Suggested Citation

  • L. Dvorská & M. Bartoš & G. Martin & W. Erler & I. Pavlík, 2001. "Strategies for differentiation, identification and typing of medically important species of mycobacteria by molecular methods," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 46(11-12), pages 309-328.
    • Handle: RePEc:caa:jnlvet:v:46:y:2001:i:11-12:id:7890-vetmed
    DOI: 10.17221/7890-VETMED
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    Citations

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    Cited by:

    1. M. Bartos & J. O. Falkinham III & I. Pavlik, 2004. "Mycobacterial catalases, peroxidases, and superoxide dismutases and their effects on virulence and isoniazid-susceptibility in mycobacteria - a review," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 49(5), pages 161-170.
    2. I. Pavlik & F. Bures & P. Janovsky & P. Pecinka & M. Bartos & L. Dvorska & L. Matlova & K. Kremer & D. Van Soolingen, 2002. "The last outbreak of bovine tuberculosis in cattle in the Czech Republic in 1995 was caused by Mycobacterium bovis subspecies caprae," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 47(9), pages 251-263.
    3. L. Matlova & L. Dvorska & J. Bartl & M. Bartos & W.Y. Ayele & M. Alexa & I. Pavlik, 2003. "Mycobacteria isolated from the environment of pig farms in the Czech Republic during the years 1996 to 2002," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 48(12), pages 343-357.
    4. L. Novotny & L. Dvorska & A. Lorencova & V. Beran & I. Pavlik, 2004. "Fish: a potential source of bacterial pathogens for human beings," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 49(9), pages 343-358.
    5. J. Hosek & P. Svastova & M. Moravkova & I. Pavlik & M. Bartos, 2006. "Methods of mycobacterial DNA isolation from different biological material: a review," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 51(5), pages 180-192.
    6. P. Lescenko & L. Matlova & L. Dvorska & M. Bartos & O. Vavra & S. Navratil & L. Novotny & I. Pavlik, 2003. "Mycobacterial infection in aquarium fish," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 48(3), pages 71-78.
    7. M. Bartos & H. Pavlikova & L. Dvorska & R. Horvath & M. Dendis & P. Flodr & Z. Kolar & R.T. Weston & L. Pac & L. Matlova & I. Pavlik, 2006. "Risk assessment of mycobacterial infections (human tuberculosis and avian mycobacteriosis) during anatomical dissection of cadavers," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 51(5), pages 311-319.
    8. I. Pavlik & L. Matlova & L. Dvorska & J. Bartl & L. Oktabcova & J. Docekal & I. Parmova, 2003. "Tuberculous lesions in pigs in the Czech Republic in the years 1990-1999: occurrence, causal factors and economic losses," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 48(5), pages 113-125.
    9. I. Pavlik & L. Dvorska & M. Bartos & I. Parmova & I. Melicharek & A. Jesenska & M. Havelkova & M. Slosarek & I. Putova & G. Martin & W. Erler & K. Kremer & D. Van Soolingen, 2002. "Molecular epidemiology of bovine tuberculosis in the Czech Republic and Slovakia in the period 1965-2001 studied by spoligotyping," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 47(7), pages 181-194.
    10. M. Skoric & E.J. Shitaye & R. Halouzka & P. Fictum & I. Trcka & M. Heroldova & E. Tkadlec & I. Pavlik, 2007. "Tuberculous and tuberculoid lesions in free living small terrestrial mammals and the risk of infection to humans and animals: a review," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 52(4), pages 144-161.
    11. K. Hruska, 2004. "Research on paratuberculosis: Analysis of publications 1994-2004," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 49(8), pages 271-282.

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