IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjs/v57y2012i5id5919-cjas.html
   My bibliography  Save this article

Innate defense capability of challenged primary bovine mammary epithelial cells after an induced negative energy balance in vivo

Author

Listed:
  • K. Danowski

    (Physiology Weihenstephan, Technical University Munich, Freising-Weihenstephan, Germany
    ZIEL - Research Centre for Nutrition and Food Sciences, Technical University Munich, Freising-Weihenstephan, Germany)

  • D. Sorg

    (Physiology Weihenstephan, Technical University Munich, Freising-Weihenstephan, Germany
    ZIEL - Research Centre for Nutrition and Food Sciences, Technical University Munich, Freising-Weihenstephan, Germany)

  • J. Gross

    (Animal Nutrition, Technical University Munich, Freising-Weihenstephan, Germany)

  • H.H.D. Meyer

    (Physiology Weihenstephan, Technical University Munich, Freising-Weihenstephan, Germany
    ZIEL - Research Centre for Nutrition and Food Sciences, Technical University Munich, Freising-Weihenstephan, Germany)

  • H. Kliem

    (Physiology Weihenstephan, Technical University Munich, Freising-Weihenstephan, Germany
    ZIEL - Research Centre for Nutrition and Food Sciences, Technical University Munich, Freising-Weihenstephan, Germany)

Abstract

Negative energy balance (NEB), if followed by metabolic imbalance, is a common problem in high-yielding dairy cows frequently associated with inflammation of the mammary gland. After entering the teat canal, mammary epithelium is the first line of defense against a pathogen invasion. To investigate the effect of NEB on the innate host defense of the mammary epithelium, primary bovine mammary epithelial cell (pbMEC) cultures were generated by cell extraction of milk derived from energy restricted and control feeding cows. pbMEC were obtained from 8 high-yielding dairy cows affected by induced NEB in mid-lactation due to a reduction to 51 ± 2% of total energy requirement (restriction group) and from 7 control cows (control group). They were exposed to heat-inactivated Escherichia coli and Staphylococcus aureus for 24 and 72 h to investigate the influence of NEB on gene expression profiles of cytokines, chemokines, genes associated with apoptosis and antimicrobial peptides plus their receptors (AMPR) of the innate immune response. The immune challenge of pbMEC demonstrated an effect of immune capacity and NEB in 15 differential expressed genes. NEB induced a substantial up-regulation in restriction compared to control cells by trend in E. coli and a down-regulation in S. aureus exposed cells. Our investigations showed that the dietary-induced NEB in vivo influenced the immune response of pbMEC in vitro and altered the expression of immunological relevant genes due to a difference in energy supply. These results demonstrate that pbMEC are a suitable model for mastitis research, in which even effects of feeding regimes can be displayed.

Suggested Citation

  • K. Danowski & D. Sorg & J. Gross & H.H.D. Meyer & H. Kliem, 2012. "Innate defense capability of challenged primary bovine mammary epithelial cells after an induced negative energy balance in vivo," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 57(5), pages 207-219.
    • Handle: RePEc:caa:jnlcjs:v:57:y:2012:i:5:id:5919-cjas
    DOI: 10.17221/5919-CJAS
    as

    Download full text from publisher

    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/5919-CJAS.html
    Download Restriction: free of charge
    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/5919-CJAS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/5919-CJAS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. Michael Zasloff, 2002. "Antimicrobial peptides of multicellular organisms," Nature, Nature, vol. 415(6870), pages 389-395, January.
    2. O. Wellnitz & P. Reith & S.C. Haas & H.H.D. Meyer, 2006. "Immune relevant gene expression of mammary epithelial cells and their influence on leukocyte chemotaxis in response to different mastitis pathogens," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 51(4), pages 125-132.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Daniel P. Roberts & Autar K. Mattoo, 2018. "Sustainable Agriculture—Enhancing Environmental Benefits, Food Nutritional Quality and Building Crop Resilience to Abiotic and Biotic Stresses," Agriculture, MDPI, vol. 8(1), pages 1-24, January.
    2. Li Liu & Ying Fang & Qingsheng Huang & Jianhua Wu, 2011. "A Rigidity-Enhanced Antimicrobial Activity: A Case for Linear Cationic α-Helical Peptide HP(2–20) and Its Four Analogues," PLOS ONE, Public Library of Science, vol. 6(1), pages 1-8, January.
    3. Erik Hartman & Fredrik Forsberg & Sven Kjellström & Jitka Petrlova & Congyu Luo & Aaron Scott & Manoj Puthia & Johan Malmström & Artur Schmidtchen, 2024. "Peptide clustering enhances large-scale analyses and reveals proteolytic signatures in mass spectrometry data," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    4. Giuseppe Maccari & Mariagrazia Di Luca & Riccardo Nifosí & Francesco Cardarelli & Giovanni Signore & Claudia Boccardi & Angelo Bifone, 2013. "Antimicrobial Peptides Design by Evolutionary Multiobjective Optimization," PLOS Computational Biology, Public Library of Science, vol. 9(9), pages 1-12, September.
    5. Larrañaga, Patricia & Díaz-Dellavalle, Paola & Cabrera, Andrea & Alem, Diego & Leoni, Carolina & Almeida-Souza, André Luis & Giovanni-De-Simone, Salvatore & Dalla-Rizza, Marco, 2012. "Activity of Naturally Derived Antimicrobial Peptides against Filamentous Fungi Relevant for Agriculture," Sustainable Agriculture Research, Canadian Center of Science and Education, vol. 1(2).
    6. Jiayang Xie & Min Zhou & Yuxin Qian & Zihao Cong & Sheng Chen & Wenjing Zhang & Weinan Jiang & Chengzhi Dai & Ning Shao & Zhemin Ji & Jingcheng Zou & Ximian Xiao & Longqiang Liu & Minzhang Chen & Jin , 2021. "Addressing MRSA infection and antibacterial resistance with peptoid polymers," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    7. Eduardo F Vicente & Luis Guilherme M Basso & Graziely F Cespedes & Esteban N Lorenzón & Mariana S Castro & Maria José S Mendes-Giannini & Antonio José Costa-Filho & Eduardo M Cilli, 2013. "Dynamics and Conformational Studies of TOAC Spin Labeled Analogues of Ctx(Ile21)-Ha Peptide from Hypsiboas albopunctatus," PLOS ONE, Public Library of Science, vol. 8(4), pages 1-12, April.
    8. Abdullah Kaviani Rad & Angelika Astaykina & Rostislav Streletskii & Yeganeh Afsharyzad & Hassan Etesami & Mehdi Zarei & Siva K. Balasundram, 2022. "An Overview of Antibiotic Resistance and Abiotic Stresses Affecting Antimicrobial Resistance in Agricultural Soils," IJERPH, MDPI, vol. 19(8), pages 1-27, April.
    9. Matthijs P. Hoelscher & Joachim Forner & Silvia Calderone & Carolin Krämer & Zachary Taylor & F. Vanessa Loiacono & Shreya Agrawal & Daniel Karcher & Fabio Moratti & Xenia Kroop & Ralph Bock, 2022. "Expression strategies for the efficient synthesis of antimicrobial peptides in plastids," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    10. Carlos Polanco González & Marco Aurelio Nuño Maganda & Miguel Arias-Estrada & Gabriel del Rio, 2011. "An FPGA Implementation to Detect Selective Cationic Antibacterial Peptides," PLOS ONE, Public Library of Science, vol. 6(6), pages 1-7, June.
    11. Guilherme D Brand & Mariana T Q Magalhães & Maria L P Tinoco & Francisco J L Aragão & Jacques Nicoli & Sharon M Kelly & Alan Cooper & Carlos Bloch Jr, 2012. "Probing Protein Sequences as Sources for Encrypted Antimicrobial Peptides," PLOS ONE, Public Library of Science, vol. 7(9), pages 1-14, September.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjs:v:57:y:2012:i:5:id:5919-cjas. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.