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Characterization and proteomic profile of extracellular vesicles from peritoneal dialysis efflux

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Listed:
  • Laura Carreras-Planella
  • Jordi Soler-Majoral
  • Cristina Rubio-Esteve
  • Sara Inés Lozano-Ramos
  • Marcella Franquesa
  • Josep Bonet
  • Maria Isabel Troya-Saborido
  • Francesc Enric Borràs

Abstract

Peritoneal Dialysis (PD) is considered the best option for a cost-effective mid-term dialysis in patients with Chronic Renal Failure. However, functional failure of the peritoneal membrane (PM) force many patients to stop PD treatment and start haemodialysis. Currently, PM functionality is monitored by the peritoneal equilibration test, a tedious technique that often show changes when the membrane damage is advanced. As in other pathologies, the identification and characterization of extracellular vesicles (EVs) in the peritoneal dialysis efflux (PDE) may represent a non-invasive alternative to identify biomarkers of membrane failure. Using size-exclusion chromatography, we isolated EVs from PDE in a group of patients. Vesicles were characterized by the presence of tetraspanin markers, nanoparticle tracking analysis profile, cryo-electron microscopy and mass spectrometry. Here, we report the isolation and characterization of PDE-EVs. Based on mass spectrometry, we have found a set of well-conserved proteins among patients. Interestingly, the peptide profile also revealed remarkable changes between newly enrolled and longer-treated PD patients. These results are the first step to the identification of PDE-EVs based new markers of PM damage, which could support clinicians in their decision-making in a non-invasive manner.

Suggested Citation

  • Laura Carreras-Planella & Jordi Soler-Majoral & Cristina Rubio-Esteve & Sara Inés Lozano-Ramos & Marcella Franquesa & Josep Bonet & Maria Isabel Troya-Saborido & Francesc Enric Borràs, 2017. "Characterization and proteomic profile of extracellular vesicles from peritoneal dialysis efflux," PLOS ONE, Public Library of Science, vol. 12(5), pages 1-20, May.
    • Handle: RePEc:plo:pone00:0176987
    DOI: 10.1371/journal.pone.0176987
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    References listed on IDEAS

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    1. Catrin Treharne & Frank Liu & Murat Arici & Lydia Crowe & Usman Farooqui, 2014. "Peritoneal Dialysis and In-Centre Haemodialysis: A Cost-Utility Analysis from a UK Payer Perspective," Applied Health Economics and Health Policy, Springer, vol. 12(4), pages 409-420, August.
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