IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0165148.html
   My bibliography  Save this article

Nonlinear Structured Illumination Using a Fluorescent Protein Activating at the Readout Wavelength

Author

Listed:
  • Hui-Wen Lu-Walther
  • Wenya Hou
  • Martin Kielhorn
  • Yoshiyuki Arai
  • Takeharu Nagai
  • Michael M Kessels
  • Britta Qualmann
  • Rainer Heintzmann

Abstract

Structured illumination microscopy (SIM) is a wide-field technique in fluorescence microscopy that provides fast data acquisition and two-fold resolution improvement beyond the Abbe limit. We observed a further resolution improvement using the nonlinear emission response of a fluorescent protein. We demonstrated a two-beam nonlinear structured illumination microscope by introducing only a minor change into the system used for linear SIM (LSIM). To achieve the required nonlinear dependence in nonlinear SIM (NL-SIM) we exploited the photoswitching of the recently introduced fluorophore Kohinoor. It is particularly suitable due to its positive contrast photoswitching characteristics. Contrary to other reversibly photoswitchable fluorescent proteins which only have high photostability in living cells, Kohinoor additionally showed little degradation in fixed cells over many switching cycles.

Suggested Citation

  • Hui-Wen Lu-Walther & Wenya Hou & Martin Kielhorn & Yoshiyuki Arai & Takeharu Nagai & Michael M Kessels & Britta Qualmann & Rainer Heintzmann, 2016. "Nonlinear Structured Illumination Using a Fluorescent Protein Activating at the Readout Wavelength," PLOS ONE, Public Library of Science, vol. 11(10), pages 1-14, October.
    • Handle: RePEc:plo:pone00:0165148
    DOI: 10.1371/journal.pone.0165148
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0165148
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0165148&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0165148?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Maria Ingaramo & Andrew G. York & Eric J. Andrade & Kristin Rainey & George H. Patterson, 2015. "Two-photon-like microscopy with orders-of-magnitude lower illumination intensity via two-step fluorescence," Nature Communications, Nature, vol. 6(1), pages 1-7, November.
    2. Tim Grotjohann & Ilaria Testa & Marcel Leutenegger & Hannes Bock & Nicolai T. Urban & Flavie Lavoie-Cardinal & Katrin I. Willig & Christian Eggeling & Stefan Jakobs & Stefan W. Hell, 2011. "Diffraction-unlimited all-optical imaging and writing with a photochromic GFP," Nature, Nature, vol. 478(7368), pages 204-208, October.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Yueli Yang & Xueyang Bai & Fanghao Hu, 2024. "Photoswitchable polyynes for multiplexed stimulated Raman scattering microscopy with reversible light control," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0165148. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.