IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v478y2011i7368d10.1038_nature10497.html
   My bibliography  Save this article

Diffraction-unlimited all-optical imaging and writing with a photochromic GFP

Author

Listed:
  • Tim Grotjohann

    (Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany)

  • Ilaria Testa

    (Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany)

  • Marcel Leutenegger

    (Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany)

  • Hannes Bock

    (Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany)

  • Nicolai T. Urban

    (Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany)

  • Flavie Lavoie-Cardinal

    (Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany)

  • Katrin I. Willig

    (Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany)

  • Christian Eggeling

    (Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany)

  • Stefan Jakobs

    (Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany
    University of Göttingen Medical School, Robert-Koch-Str. 40, 37075 Göttingen, Germany)

  • Stefan W. Hell

    (Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany)

Abstract

Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence capability of adjacent features on and off is making nanoscale imaging routine. Reported fluorescence nanoscopy variants switch these features either with intense beams at defined positions or randomly, molecule by molecule. Here we demonstrate an optical nanoscopy that records raw data images from living cells and tissues with low levels of light. This advance has been facilitated by the generation of reversibly switchable enhanced green fluorescent protein (rsEGFP), a fluorescent protein that can be reversibly photoswitched more than a thousand times. Distributions of functional rsEGFP-fusion proteins in living bacteria and mammalian cells are imaged at

Suggested Citation

  • Tim Grotjohann & Ilaria Testa & Marcel Leutenegger & Hannes Bock & Nicolai T. Urban & Flavie Lavoie-Cardinal & Katrin I. Willig & Christian Eggeling & Stefan Jakobs & Stefan W. Hell, 2011. "Diffraction-unlimited all-optical imaging and writing with a photochromic GFP," Nature, Nature, vol. 478(7368), pages 204-208, October.
    • Handle: RePEc:nat:nature:v:478:y:2011:i:7368:d:10.1038_nature10497
    DOI: 10.1038/nature10497
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature10497
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature10497?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Yueli Yang & Xueyang Bai & Fanghao Hu, 2024. "Photoswitchable polyynes for multiplexed stimulated Raman scattering microscopy with reversible light control," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    2. Hui-Wen Lu-Walther & Wenya Hou & Martin Kielhorn & Yoshiyuki Arai & Takeharu Nagai & Michael M Kessels & Britta Qualmann & Rainer Heintzmann, 2016. "Nonlinear Structured Illumination Using a Fluorescent Protein Activating at the Readout Wavelength," PLOS ONE, Public Library of Science, vol. 11(10), pages 1-14, October.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:478:y:2011:i:7368:d:10.1038_nature10497. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.